Next Article in Journal
First Report of Cylindrospermopsin Production by Two Cyanobacteria (Dolichospermum mendotae and Chrysosporum ovalisporum) in Lake Iznik, Turkey
Next Article in Special Issue
Protective Effect of Two Yeast Based Feed Additives on Pigs Chronically Exposed to Deoxynivalenol and Zearalenone
Previous Article in Journal
Molecular and Insecticidal Characterization of a Novel Cry-Related Protein from Bacillus Thuringiensis Toxic against Myzus persicae
Previous Article in Special Issue
Aflatoxin B1 Degradation by a Pseudomonas Strain
Article Menu

Export Article

Open AccessArticle
Toxins 2014, 6(11), 3157-3172;

Screening a Strain of Aspergillus niger and Optimization of Fermentation Conditions for Degradation of Aflatoxin B1

Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China
Some data of the manuscript has been published in the conference paper: Xue, B.B.; Shan, A.S.; Shi, B.M. Screening and identification of an aflatoxin B1-degradation strain from moldy cereals. Adv. Mater. Res. 2013, 610–613, 3461–3465. In Proceedings of the 2nd International Conference on Energy, Environment and Sustainable Development, EESD 2012, Jilin, China, 12–14 October 2012.
Authors to whom correspondence should be addressed.
Received: 13 September 2014 / Revised: 23 October 2014 / Accepted: 5 November 2014 / Published: 13 November 2014
(This article belongs to the Special Issue Detoxification of Mycotoxins)
Full-Text   |   PDF [877 KB, uploaded 13 November 2014]   |  


Aflatoxin B1, a type of highly toxic mycotoxin produced by some species belonging to the Aspergillus genus, such as Aspergillus flavus and Aspergillus parasiticus, is widely distributed in feed matrices. Here, coumarin was used as the sole carbon source to screen microorganism strains that were isolated from types of feed ingredients. Only one isolate (ND-1) was able to degrade aflatoxin B1 after screening. ND-1 isolate, identified as a strain of Aspergillus niger using phylogenetic analysis on the basis of 18S rDNA, could remove 26.3% of aflatoxin B1 after 48 h of fermentation in nutrient broth (NB). Optimization of fermentation conditions for aflatoxin B1 degradation by selected Aspergillus niger was also performed. These results showed that 58.2% of aflatoxin B1 was degraded after 24 h of culture under the optimal fermentation conditions. The aflatoxin B1 degradation activity of Aspergillus niger supernatant was significantly stronger than cells and cell extracts. Furthermore, effects of temperature, heat treatment, pH, and metal ions on aflatoxin B1 degradation by the supernatant were examined. Results indicated that aflatoxin B1 degradation of Aspergillus niger is enzymatic and this process occurs in the extracellular environment. View Full-Text
Keywords: Aspergillus niger; coumarin; aflatoxin B1; degradation Aspergillus niger; coumarin; aflatoxin B1; degradation

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Zhang, W.; Xue, B.; Li, M.; Mu, Y.; Chen, Z.; Li, J.; Shan, A. Screening a Strain of Aspergillus niger and Optimization of Fermentation Conditions for Degradation of Aflatoxin B1 . Toxins 2014, 6, 3157-3172.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top