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Open AccessArticle

Accumulation Kinetics of Three Scirpentriol-Based Toxins in Oats Inoculated in Vitro with Isolates of Fusarium sporotrichioides and Fusarium poae

1
Institute of Animal Nutrition, Hohenheim University, Emil-Wolff-Str. 10, 70599 Stuttgart, Germany
2
Experimental Station Ihinger Hof, Hohenheim University, 71272 Renningen, Germany
*
Author to whom correspondence should be addressed.
Toxins 2011, 3(5), 442-452; https://doi.org/10.3390/toxins3050442
Received: 24 March 2011 / Revised: 27 April 2011 / Accepted: 28 April 2011 / Published: 9 May 2011
(This article belongs to the Special Issue Trichothecenes)
Autoclaved oats were inoculated with a strain of Fusarium sporotrichioides or Fusarium poae. Moisture content of oats after inoculation was at 38%, incubation took place in standing culture at 28 °C. The A-type trichothecenes, 4,15-diacetoxyscirpenol (4,15-DAS), 15-monoacetoxyscirpenol (15-MAS), and scirpentriol (SCIRP) were analyzed by GC/MS. For each strain, three culture flasks were harvested at 2–3 day intervals starting immediately after inoculation. Total incubation time was 42 days (F. poae) and 56 days (F. sporotrichioides). Following peak accumulation, 4,15-DAS decreased below the detection limit for both strains, 15-MAS decreased below this limit for the isolate of F. sporotrichioides, for the isolate of F. poae it decreased to a level markedly below the peak value. SCIRP, after having peaked, decreased to some extent for the strain F. sporotrichioides, with a significant (P = 0.0029) negative linear regression of toxin content against culture age during this period. The content of 15-MAS, and in part also of 4,15-DAS, decreased along with an increase of SCIRP. This sequential accumulation pattern suggests the successive induction of esterases deacetylating 4,15-DAS and 15-MAS, as well as of enzymes involved in the metabolization of the parent alcohol, SCIRP. The results may explain, at least in part, the somewhat higher incidence in naturally contaminated compounds reported in the literature for SCIRP compared to 4,15-DAS and 15-MAS. View Full-Text
Keywords: Fusarium poae; Fusarium sporotrichioides; oats; scirpentriol toxins; sequential accumulation; toxigenicity; trichothecenes Fusarium poae; Fusarium sporotrichioides; oats; scirpentriol toxins; sequential accumulation; toxigenicity; trichothecenes
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Schollenberger, M.; Müller, H.-M.; Liebscher, M.; Schlecker, C.; Berger, M.; Hermann, W. Accumulation Kinetics of Three Scirpentriol-Based Toxins in Oats Inoculated in Vitro with Isolates of Fusarium sporotrichioides and Fusarium poae. Toxins 2011, 3, 442-452.

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