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Review

RETRACTED: Mycotoxin Contamination of Beverages: Occurrence of Patulin in Apple Juice and Ochratoxin A in Coffee, Beer and Wine and Their Control Methods

by
Kasa R. N. Reddy
1,*,
Hamed K. Abbas
2,*,
Craig A. Abel
3,
Wayne Thomas Shier
4 and
Baharuddin Salleh
1
1
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia
2
National Biological Control Laboratory, Crop Genetics Research Unit, Agricultural Research Service, US Department of Agriculture, 59 Lee Rd., PO Box 67, Stoneville, MS 38776, USA
3
Corn Insects and Crop Genetics Research Unit, Agricultural Research Service, US Department of Agriculture, Room 401, Genetics Building, Iowa State University, Ames, IA 50011, USA
4
Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, 308 Harvard St., SE, Minneapolis, MN 55455, USA
*
Authors to whom correspondence should be addressed.
Toxins 2010, 2(2), 229-261; https://doi.org/10.3390/toxins2020229
Submission received: 10 December 2009 / Revised: 18 January 2010 / Accepted: 21 January 2010 / Published: 2 February 2010
(This article belongs to the Special Issue Advances in Mycotoxin Research)
Versions Notes

Abstract

:
Mycotoxins are toxic secondary metabolites produced under special conditions of moisture and temperature by aerobic, microscopic fungi that can colonize a variety of foods from before harvest up to consumer use. Contamination of beverages, including that of apple juice by patulin and coffee, beer and wine by ochratoxin A has gained worldwide interest in recent years with the revelation of the effect of these toxins on human health. Patulin and ochratoxin A, which are produced by species of Penicillium, Byssochlamys and Aspergillus, pose the greatest threat to human health and are subject to government regulation in juices and beverages worldwide. This review provides an overview of the prevalence of these mycotoxins in beverages and control methods which help in establishing and carrying out proper management strategies. A detailed investigation on contamination of juices and beverages by toxic compounds helps to provide safe products for consumption and export, and they allow us to prioritize future research efforts.

1. Introduction

Beverages are among the many food product groups at risk of contamination by harmful mycotoxins. These mycotoxins may have been formed in an agricultural product before beverage manufacture, or they may be formed during manufacturing. Mycotoxins are unlikely to be formed during storage of the finished product after manufacture, because effective sterilization and storage conditions are almost always used to maximize shelf life. Mycotoxin formation during manufacturing is a particular concern with fermented beverages. Understanding the formation and fate of mycotoxins in manufactured foods is of interest because of the possible need to alter steps in the manufacturing processes either to prevent formation of mycotoxins or, more frequently, to remove them. The bulk of the research on mycotoxins in beverages has been focused on the presence of patulin in fruit juices, particularly apple juice, and on ochratoxin A (OTA) in beer, wine and coffee. These two mycotoxins will form the basis of the present review. However, there have been a variety of less extensive studies on other mycotoxins in other beverages, such as aflatoxin M1 in milk [1] and grain-derived trichothecenes and zearalenones in beer [2], which will not be covered here.
Patulin is a mycotoxin produced by several species of Penicillium, Aspergillus and Byssochlamys [3]. Due to its solubility in water it is a common contaminant of apples used for the production of apple-juice concentrates [4]. Patulin has also been found in pears, apricots, peaches, grapes and oranges, as well as products derived from these fruits [3]. Patulin is stable in an acidic environment and is not destroyed during thermal processing; therefore, it may exist in juices even after processing [5,6]. Patulin has been classified as a group 3 carcinogen, which means that there is no evidence of carcinogenicity in humans and that data in experimental animals on carcinogenesis is sparse [7], although a few studies have reported patulin to be both teratogenic and genotoxic [8,9]. Patulin toxicity is believed to be due to its reactions with sulphydryl groups, proteins and amino acids in the plasma membrane [10,11]. It is reported to cause depletion of glutathione, which may result in oxidative damage [12]. In animal studies patulin has been found to damage the gastrointestinal and respiratory systems [13], DNA and many enzymes [14]. Therefore, a provisional maximum tolerable daily intake (PMTDI) of 0.4 µg/kg body weight (b.w.) has been established [15]. Based on this PMTDI, patulin is regulated in the European Union at levels of 50 µg/kg in fruit juices and fruit nectars, 25 µg/kg in solid apple products and 10 µg/kg in apple-based products for consumption by infants and young children [16].
OTA is a mycotoxin produced, under particular environmental conditions, by fungi belonging to several species of the genera Aspergillus and Penicillium. Many studies have shown that this mycotoxin is frequently present in a series of products, particularly green coffee [17], beer [18] and wine [19]. OTA was first reported in South Africa as a secondary metabolite produced by a strain of A. ochraceus [20]. OTA is nephrotoxic, hepatotoxic, genotoxic, teratogenic and immunotoxic to animals and its carcinogenicity in rats and male mice is well-established [21]. It has been linked to Balkan Endemic Nephropathy and the development of human urinary tract tumours [22,23,24]. The International Agency for Research on Cancer has classified OTA as a possible carcinogen to humans (group 2B) [25]. The Provisional Tolerable Weekly Intake (PTWI) recommendation for OTA of 100 ng/kg b.w. [26], but the Scientific Committee on Food of the European Union has recommended a lower daily intake (5 ng/kg b.w.) [27]. Nevertheless, the Panel on Contaminants in the Food Chain (CONTAM) of the European Food Safety Authority recently derived a Tolerable Weekly Intake (TWI) of 120 ng/kg b.w for OTA [28]. Recent analyses of the dietary exposure of adult European consumers to OTA revealed that at present the weekly exposure ranges from 15 to 60 ng/kg b.w., including high consumers of foods containing OTA. In view of the above mentioned description of patulin and ochratoxin A toxic effects, it is essential to have an understanding of levels of these toxins in juices and beverages which will help in establishing and carrying out proper management strategies. Therefore, this paper reviews the occurrence of patulin in apple juice and OTA in coffee, beer and wine and methods for managing these mycotoxins.

2. Occurrence of Patulin in Apple Juice and OTA in Coffee, Beer and Wine

2.1. Occurrence of patulin in apple juice

Patulin-producing fungal strains have been isolated from a variety of fruits including apples, grapes, cherries, crab apples, pears, apricots, strawberries, nectarines, black mulberries, white mulberries, lingonberries, peaches and plums [29,30,31,32,33,34,35,36,37,38,39,40,41,42,43]. The presence of patulin-producing fungi does not necessarily guarantee patulin production and patulin production is usually, but not exclusively, associated with apple soft rot and blue mold rot, most commonly caused by P. expansum [29,33]. This fungus, which is the most common cause of apple rot in the apple industry [44] has been shown to naturally cause blue-rot in apples [30,31].
Patulin production within fruits and their products has been investigated and often appears to be dependent on factors such as water activity (aw), temperature, pH, and other chemical characteristics intrinsic to fruits [45,46]. Patulin production and pH are inversely related, with patulin being unstable at high pH [46]. Temperature has been shown to affect pathogen growth and to a greater extent, the production of patulin [46]. Patulin production has been observed at all temperatures permitting P. expansum growth, encompassing an approximate range of 0 to 30 °C [33]. Byssochlamys nivea has been shown to grow faster at temperatures of 30 and 37 °C, while patulin production was highest at 21 °C [47]. As testament to the variability seen in patulin production, even cultivar differences among apples affect the patulin production of P. expansum [46], with Jonathan, Goudreinetter, Cox’s Orange Pippin and Bramley being particularly susceptible, whereas Golden Delicious appears to be particularly resistant [48]. Apple juice and cider were once thought to be the only products to naturally contain patulin [49]. Within the food industry, apples and their respective products are of greatest concern for patulin contamination. While a variety of other food sources and products have been found with patulin and/or patulin-producer contamination, the frequency of these events is much less than that of the apple industry.
Patulin has been identified in apples and its products from various countries around the world, such as Canada [33], Chile [50], England [33,51], Finland [52], France [33,53,54], Spain [55,56,57], New Zealand [58], Netherlands [59], Sweden [60], USA [33,61,62], Turkey [63,64,65], Australia [66,67], Iran [68,69], Saudi Arabia [70], Argentina [71], Brazil [72,73,74,75,76], Japan [77], Italy [78,79,80], India [81], Austria [82], Belgium [83,84], Greece [85] and other countries, as presented in Table 1.

2.2. Occurrence of OTA in coffee

Coffee (Coffea arabica L.) is one of the most important commodities in the world’s economy. It is the second most valuable traded commodity after oil, with a traded value of $5.6 billion in 2000/2001. The total world coffee consumption is estimated to be over 6 million tonnes per annum, with Europe being the largest market, followed by the U.S., and Japan in third position. Producing countries consumed 28 million 60 kg bags in the 2003/2004-crop year, which is approximately 27% of world output as estimated by the International Coffee Organization (ICO). Recent projections for the 2005/2006 global production are in the region of 110 million bags. As far as coffee is concerned, three Aspergillus spp. − A. ochraceus, A. carbonarius and A. niger − have been reported to be responsible for OTA accumulation [88,89,90,91]. However, A. ochraceus is believed to be the major source of OTA in green coffee [92]. In a preliminary study of Vietnamese coffee beans, A. niger was the sole toxigenic species isolated and 87% of isolates produced OTA [93]. In studies of Brazilian coffee beans, over 75% of A. ochraceus and A. carbonarius isolates produced OTA, whereas only 3% of A. niger isolates were toxigenic [94]. Within the section Circumdati (yellow Aspergilli), two new ochratoxigenic species, A. westerdijkiae and A. steynii, segregated from A. ochraceus, have been described [95].
Several studies have shown a high occurrence of A. ochraceus isolates in green coffee [94,96] and a high percentage (75–90%) of isolates with a capacity to produce OTA has been detected [94]. Recently, it was suggested that A. niger and closely related species such as A. carbonarius could be OTA producers in coffee [90,93,97]. The first report on the occurrence of OTA in coffee appeared in 1974 [98]. Since then, several reports have confirmed the presence of OTA in green coffee beans [99,100,101,102], roasted coffee [103,104] and instant coffee [105,106]. OTA has also been detected in the final coffee brew prepared under normal methods [107]. OTA is the main mycotoxin reported in coffee [108] with the contamination levels ranging from 0.1 to 80 ng/g in green coffee [100].
Table 2. OTA levels reported in coffee in different countries around the world.
Table 2. OTA levels reported in coffee in different countries around the world.
CountryType of coffeeOTA level
(range µg or ng/kg)		Reference
Africa-various
countries		Robusta2.4-23.3[124,125]
ArabiaGreen0.73-34.08[118]
AustraliaSoluble coffee0.2-4.0[126]
BrazilGreen, Instant, Roasted0.1-6.5[127,128,129,130,131,132]
CameroonRobustaTraces-2.2[133]
CanadaRoasted0.1-2.3[106]
ColombiaArabica0-3.3[133]
DenmarkRoasted0.1-3.2[134]
East AfricaNot specified0.2-62.0[125]
EthiopiaArabica<0.1[100]
GermanyRoasted0.21-12.1[135,136]
HungaryRoasted0.17-1.3[137]
IndiaGreen0.2-13.5[17,138]
IndonesiaRobusta0.2-1.0[100]
JapanRaw and Instant0.16-1.1[139]
KenyaArabica<0.01[133]
MexicoArabica1.4[133]
SpainGreen0.67[124]
SwitzerlandGreen0.4-7.8[103]
TaiwanGreen0.1-0.5[140]
TanzaniaNot specified2.2[103]
USARoasted0.1-1.2[141]
UgandaNot specifiedTrace[98]
YemenArabica0.7-17.4[100]
ZaireRobusta8.4-15.0[142]
Extensive sampling of green coffee from all origins and both types of coffees (arabica and robusta) has shown that OTA contamination may be more frequent in some areas, but no producing country is entirely free from contamination [109]. In Brazil, the presence of OTA in coffee beans has been vigorously evaluated with contamination levels varying significantly [94,110,111,112,113]. OTA has been identified in coffee from various countries around the World, such as Australia [114], France [115,116], India [17], Netherlands [117], Saudi Arabia [118], Switzerland [119,120], and USA [121]. In summary, OTA is found at different stages of coffee production and processing, including cherry, green coffee and roasted coffee [94,101,105,122,123] and in many countries, as presented in Table 2.
Nine countries have specific regulations for OTA, with legislative limits ranging from 5 to 50 µg/kg. If a maximum limit for OTA in coffee were to be established, it could affect international trade for producing countries which do not control this parameter. Without such limits (uncontrolled) this could have an impact on human health in coffee importing countries. The occurrence of OTA in coffee beans can be due to both environmental conditions (climate, length of storage and transportation) and processing conditions (wet, mechanical or dry processes) [100]. The occurrence and the formation of OTA in the dry process have been studied by several authors [96,112]. OTA was present before storage, indicating the possibility that harvesting and post-harvest handling of coffee cherries could be the critical steps leading to contamination [96]. There is currently little information available on the presence of OTA producing fungi in coffee beans used in the wet and mechanical processes and the impact of these processes on the production or presence of OTA.

2.3. Occurrence of OTA in beer

Beer, a beverage derived from cereals, mainly barley, is widely consumed around the world. In 2004, beer consumption exceeded 150 billion litres; the higher consuming regions were Europe (32.8%), Asia (28.7%), North America (17.4%) and Central/South America (14.4%) [143]. OTA is typically carried with the contaminated commodities, mainly malting barley, but perhaps also with the adjuncts, to breweries. Brewing processes vary from one industry to another, but the way the toxin is carried over into the beer is basically the same. OTA is stable through the boiling process. After mashing, some OTA is recovered in the spent grains, but wort (the extracted liquid) does contain OTA. After fermentation, yeasts retain part of the original OTA content and the remainder is transferred to the beer [2]. Until the 1990s, very few reports are available on the natural occurrence of OTA in beer [144,145]. Today, surveys conducted in various countries have shown that OTA is a common contaminant of beer due to the presence of ochratoxigenic strains of P. verrucosum or A. ochraceus in grains. At present, there is no maximum allowable limit (MAL) established for OTA in beer by the European Union (EU) regulations, although the limit for cereals destined to human consumption is 3 ng/g.
In Spain, the first report on OTA occurrence in beer was published by Legarda and Burdaspal [146]. In Italy, Visconti et al. [147] found OTA in 30 out of 61 beer samples, with no substantial differences between strong and light beers. The results of Bacaloni et al. [148] agreed with these data. In Germany, the first results were obtained with methods having a relatively high limit of detection, so consequently, the frequency of positive samples (42.5%) was low, although values as high as 1.53 ng/mL were reported. In Belgium, Tangni et al. [149] showed that OTA occurred in 97% and 100% of domestic and imported beers, respectively; however, levels were < 0.2 ng/mL and comparable to levels found in other European countries. Nakajima et al. [150] found OTA in 12 out of 15 Belgian beers imported in Japan. Jorgensen [134] found that all 21 analyzed Danish beer samples were contaminated with OTA (0.001–0.160 ng/mL). Peito and Venancio [151] reported that 13% of domestic and 60% of imported beers in Portugal were contaminated with this mycotoxin. Varga et al. [152] reported 0.25 ng/mL level of OTA in one Hungarian beer sample.
In the US, 130 breweries were checked by Fischbach and Rodricks [145] and found to contain OTA up to 10 μg/kg. Nakajima et al. [150] found 100% of 17 samples in US were contaminated (0.002-0.0311 ng/mL). OTA was found in 26 out of 41 samples of Canadian beers [153] and in eight out of 10 samples from Central and South America [150]. It was only detected in trace amounts in six out of 26 samples of Brazilian beer [154]. In Turkey, Gumus et al. [155] found OTA in 42 out of 150 samples. In Japan, Nakajima et al. [150] detected OTA in 21 out of 22 beer samples at levels ranging from 0.0022 to 0.0448 ng/mL whereas Sugita-Konishi et al. [18] detected OTA in 12 out of 20 samples, which matches the previous report. Thirteen samples from other Asian countries were screened and the toxin was found in practically all of them. In South Africa, Odhav and Naicker [156] found very high OTA levels (3-2340 ng/mL) in traditional breve beers (13 out of 29). The upper value is the highest level ever reported for OTA content in beer, whereas the toxin was not detected in Moroccan beers [157]. Thus, it may be concluded that OTA occurs in beer all over the world, with more than 50% of analyzed samples showing detectable levels, although they were usually <0.2 ng/mL. This means that beer is not a significant contributor to population exposure to OTA (<3% with respect to the total world intake of 100 ng/kg b.w.). As yet there is no limit prescribed for this beverage; hence, high OTA content in beer cannot be regulated. A few other reports of OTA found in beer are presented in Table 3.

2.4. Occurrence of OTA in wine

Wine is an important beverage in world trade. France, Italy, Spain and the US are the main producing countries, followed by Argentina, China and Australia. France, Italy and Spain are the main wine exporters. In 2004, France was the highest consuming country, followed by Italy [166]. After the first report on the occurrence of OTA in wine [167], several surveys were conducted to assess the prevalence of this mycotoxin in wine and grape products [168]. The results of a total of 1,706 wine samples analysed are summarised in Battilani et al. [169] and two further surveys were also recently conducted in Spain [170,171].
A focus of studies on OTA contamination of wines has been to identify what types of wine are most susceptible to contamination by the mycotoxin. If a particular type of grape or step in the manufacturing process could be associated with OTA contamination, it might be possible to alter the process or type of product to avoid marketing contaminated products. Attention has focussed on two areas: dessert wines and red wines. The rationale for focussing on dessert wines is that they are manufactured from grapes grown in warmer climates, which have a higher sugar content that allows optimal ethanol production while still leaving enough sugar to give the sweetness characteristic of dessert wines. Thus, grapes grown in warmer climates are more likely to be contaminated with mycotoxins, and OTA contaminating the fruit would be expected to contaminate wine made from it. Red wines have been a focus of attention because the red colour is extracted from the skins of red grapes during the fermentation step. It was suspected that Aspergillus and Penicillium species that contaminated skins before harvest might produce OTA during the fermentation step. If this mechanism were shown to be an important source of OTA, it would be possible for vintners to avoid losses by preparing white wines from red grapes (i.e., by removing and discarding the skins before fermentation) in cases when grapes had been identified on the vine as having skins contaminated with Aspergillus and Penicillium species.
The first data on OTA occurrence in wine marketed in Spain are from Burdaspal and Legarda [172]. Most of their samples were domestic, but some of them were imported. Dessert wines showed the highest incidence of contamination (about 73% of samples) followed by rosé, red and white wines, in that order. Further surveys have reported [170,171,173,174,175] that OTA contamination in wine. The overall % of contaminated samples was 51.5%. The highest OTA concentration was 15.25 ng/mL in dessert wine, while in other wines the highest levels were < 4.5 ng/mL. Dessert wines are prone to contamination with OTA as in the case of Spanish wines [147,176,177]. In Greece, more than 66% of wine samples showed detectable OTA levels and both red and sweet wines showed the highest levels [178,179,180]. More than 50% of the samples analyzed in Cyprus and Turkey, respectively, had detectable levels of the toxin [181,182].
In general, OTA content of wines correlates with colour, with a decrease from red to rose and to white, as has been shown by several studies [166,170,176]. Usually red wines have higher levels of OTA contamination than white wines [183], which may be due to the increased time of contact between berry skins and grape juice during the mashing stage [184]. However, Stefanaki et al. [180] found that the OTA concentration in red dry wines was not significantly different from that found in white and pink wines in Greece. In Italy, wines have been extensively surveyed for this toxin [185,186]. OTA incidence was higher in red wines (78.4%), followed by rose and dessert and white wines. The highest level (7.63 ng/mL) was found in red wine. In Germany, a value of 7.0 ng/mL was found in Italian red wine exported to Germany [184,187].
In France, Ospital et al. [188] found OTA in 29 samples of wines (0.01–0.27 ng/mL) but a value of 0.78 ng/mL was found in a French red wine exported to Germany. In Portugal, Festas et al. [189] did not find OTA in 64 domestic wines, but Soleas et al. [160] detected it in five out of 37 samples of Portuguese wine. A survey of 340 Portuguese wines revealed that OTA was detectable in 20.3% of the samples and the highest level was 2.1 ng/mL [190]. However, a level of 15.6 ng/mL was reported in red wine from southern Europe [191]. Siantar et al. [192] found that 69 out of 84 US wines contained < 0.01 ng/mL and the remaining contained < 1 ng/mL. Soleas et al. [160] found OTA in 16.6% of 580 red wine samples and in 3.9% of 362 white wine samples marketed in Canada but were unable to detect the toxin in their US samples.
Wines from North America had lower OTA levels than European wines [193]. Rosa et al. [194] detected OTA in 24% of 42 wine samples from Brazil, Argentina and Chile (0.02–0.07 ng/mL). However, Soleas et al. [160] found OTA in Argentinean wines. Australian wines were included in several surveys. Most samples contained < 0.05 ng/mL and the highest level was 0.62 ng/mL [195]. Sugita-Konishi et al. [18] studied wines commercialized in Japan and found that 6 out of 10 wines had detectable OTA levels (0.07–0.72 ng/mL). In South Africa, Shephard et al. [196] detected the toxin in 24 local samples. There the highest level (2.67 ng/mL) was found in noble wine by Stander and Steyn [197]. Filali et al. [157] found OTA in 30 wine samples from Morocco. Some additional reports of OTA found in wine as presented in Table 4. Wine is considered the major source of OTA intake after cereals [191].

3. Patulin Control Methods

During the manufacture of apple juice, various treatments of a physical, mechanical, chemical or biological nature can be used to control or reduce apple rot and the levels of patulin in the final product. The efficiency of each of these measures depends on the technique used and on the skill and diligence with which it is applied. The following discussion will focus on the stages of apple juice manufacture that are recognised to control, increase or reduce levels of patulin, as well as those that require
more research.

3.1. During apple harvest, processing and storage

Apples are typically harvested and processed, and apples found to be unfit for marketing as top quality edible retail apples are stored in controlled atmosphere, refrigerated environments for anywhere up to around 12 months when the next harvest comes in. Measures taken to prevent patulin synthesis during preproduction are based upon fruit quality and facility sanitation measures. The quality of fruit resulting from harvesting is the first step in controlling patulin levels. With the highest quality, hand-picked fruit being used for direct retail sale, processed apple products usually are produced from mechanical harvest, windfalls, insect-damaged, or culled fruit. Bruises, skin breaks and other physical damage within these apples provide a perfect entry for P. expansum and other patulin-producing fungal species into the fruit [205]. Studies have examined the effect of fruit quality and harvest method on the patulin content of the resultant juices. In a study, patulin was undetectable in cider from seven cultivars when fruit was tree-picked cider, whereas it was detected between 40.2 and 374 µg/L in cider from four cultivars when fruit was ground-harvested cider [206]. Many of the patulin control measures suggested by the Joint FAO/WHO Food Standards Programme are based upon the careful selection of fruits as good agricultural practice [207].
Standard post-harvest processing, including washing, sorting and packaging, poses a second means to control both fungal and mycotoxin contamination. High-pressure wash with water has been shown to reduce patulin levels in apple juice by 21% to 54% [5]. Another study showed that washing ground-harvested apples resulted in 10% to 100% patulin reduction, depending on the initial patulin level and the type of washing treatment [208]. However, these same washes can also serve as a source of contamination. Contaminated bins, storage rooms, drencher washes, drying brushes after apple wash and other steps within the processing cycle can all provide a source of fungal inoculum. Prevention methods aimed at cleaning and sterilizing storage and processing facilities routinely and in between seasons are being mapped out, but have not yet been fully developed. Plus, the older, complicated design of many packing-houses and processing equipment inhibits the ability to effectively sanitize. Even if effective strategies are successfully mapped out, the inherent variability in apple handling facilities will require customization of sanitation methods for each operation [44], posing a considerable cost to producers.
Apple storage poses yet a third means of fungal control and contamination. Conflicting evidence exists as to whether standard controlled-atmosphere, refrigerated storage is sufficient to prevent apple soft rot [33,209,210]. Blue-rot infections at the stem-end, which was classically associated with wounded fruit, began to appear with increasing frequency in non-wounded fruit in the mid-1990s. Long-term, controlled atmosphere storage has now been shown to allow the slow growth and stem-based invasion of fungi into apples [44]. Furthermore, these facilities are not available to all producers, forcing many to use deck-storage, which can drastically increase patulin production. One alternative to room storage is the use of packaging materials such as polyethylene, which through their own atmospheric control, have been shown to reduce patulin production in apples [211]. In the same study mentioned previously [206], patulin was not detected in cider from tree-picked apples stored 4 to 6 weeks at 0 to 2 °C, but was detected at levels between 0.97 and 64 µg/Lin stored, tree-picked, unculled fruit. Cider from apples stored in a controlled atmosphere and culled showed 0 to 15.1 µg/L patulin while unculled apple fruit yielded 59.9 to 120.5 µg/Lpatulin.
The longer the storage of the apples, the greater the risk of increased patulin content. This is particularly evident to juice producers within the U.K. who see dramatic rises of patulin content in juice produced in June, July, and August- the months just prior to the new harvest season where source apples have been stored for almost a year [48]. Furthermore, even if apples are perfectly processed prior to storage, addition of diphenylamine, which is used to treat ‘storage scald’ in stored apples, can provide an inoculum of patulin-producing fungi in the fruit [44]. Various fungicides and other treatments have been investigated for the ability to reduce apple rot and patulin production at all processing steps. Postharvest treatment of apples with benzimidazole fungicides was used from the 1970s through the early 1990s but has since been largely abandoned due to fungal resistance [44]. Apples inoculated with P. expansum and heated at 38 °C for 4 days showed no decay lesions after storage at 1 °C for 6 months. After heat-treatment, inoculation with P. expansum followed by infusion of 2% calcium chloride alone and Pseudomonas syringae treatment alone reduced decay incidence by 25% each. Calcium treatment plus Pseudomonas syringae treatment without and with subsequent heat treatment reduced decay by 89% and 91%, respectively [212]. Finally, other methods used for the control of human pathogens like Escherichia coli, such as washing with solutions of peroxyacetic acid, chlorine dioxide, and chlorine [213,214,215], may also provide some benefit toward preventing postharvest apple decay.

3.2. During juice production: preparation for crushing, filtering, clarification and pasteurization

For removal of patulin during both production and post production, effective decontamination/detoxification procedures must: (1) inactivate, destroy, or remove the toxin, (2) not produce or leave new toxic substances, (3) retain nutritive value/acceptability of the product, (4) not significantly alter the technological processes associated with the product, and (5) if possible, destroy fungal spores.
Methods of patulin control within standard apple juice production steps are centred on three of the areas listed above. The first of these involves the quality of the fruit and processing of the fruit that goes to pressing. As previously mentioned, processed apple products are often made from lower quality fruit that is unsuitable for direct market retail. Removal of decayed/damaged fruit or trimming of moldy portions can significantly reduce patulin levels in apple products [216,217,218,219,220]. Trimming of rotten sections of apple has been shown to remove up to 99% of patulin contamination [209]. However, this process is expensive and labour intensive. Furthermore, patulin can be detected in visibly sound fruit [206] and can spread from rotten areas of apples into sound areas [220]. Two studies have shown that patulin could diffuse 1 to 2 cm from the rotten core in apples [217,221]. Producers’ tests on apples have shown that the patulin level is often not related to the physical quality of the fruit, with both high-rot fruit and top-quality eating fruit often having high levels of patulin [48]. While often beneficial, the sorting of decayed apples to the level of being effective is difficult to impossible, often necessitating the need to reject entire loads of juice from contaminated apples. If reliant on this method, small-scale producers who cannot afford these losses will be forced to sort by hand to the point that many may cease business operations [44].
The second area of standard juice production capable of reducing patulin levels involves the juice clarification process. Results from this process have been mixed, and those methods most successful at removing patulin often do so at the expense of juice sensory and authenticity qualities. Some sources suggest that standard fruit juice production processes remove only about 20% of patulin [4,222]. A study conducted by Acar et al. [5], showed that the traditional apple juice production processes of depectinization, clarification, and filtration through a rotary vacuum precoat filter could reduce patulin levels by 39%. In the same study, use of depectinization, clarification, mixing with gelatin/bentonite and ultrafiltration resulted in a 25% decrease in patulin. Another study by Artik et al. [223] examined the effects of gelatin/bentonite flocculation, filtration, activated charcoal, ultrafiltration, polyvinylpolypyrrolidone, and polystyrene-divinyl benzene (DVB)-based macro porous resin on apple juice colour, clarity, phenolic content, organic acid content, and patulin reduction. Activated charcoal treatment had the highest patulin reduction with 40.9% but also significantly reduced colour and phenolic content, two characteristics associated with juice authenticity. Polystyrene-DVB-based macro porous resin was the second most effective, reducing patulin by 11%. None of the treatments altered organic acid content significantly. Centrifugation and fining of juice pulp have been shown to reduce patulin levels by 89% and 77%, respectively. However, these methods potentially make the filter cake that is removed highly toxic and unfit for any further use, such as animal feed [224]. This could represent a loss of an important income stream for many juice producers.
Another juice production process also capable of reducing patulin levels is the pasteurization process. Of the three processes mentioned thus far, this is by far the least effective. Repeated studies have shown that, while unstable at high pH [46], patulin is relatively stable to thermal degradation in the pH range of 3.5 to 5.5, with lower pH leading to greater stability [225,226]. The half-life of patulin held at 25 °C at pH 6.0 and 8.0 has been shown to be 55 and 2.6 days respectively [227]. In another study, no reduction of patulin occurred during concentration and pasteurization [228], while another study did show a 50% reduction of patulin in apple juice treated for 20 min at 80 °C [229] a much longer treatment than used in standard pasteurization procedures. In addition, studies by Wheeler et al. [230] showed pasteurization treatments between 60 and 90 °C for 10 seconds were only able to reduce patulin levels by 18.8%. Evidence also shows that patulin is non-volatile and upon distillation, in order to produce apple aroma, patulin remains within the juice concentrate [231]. Finally, not only is the pasteurization process unable to significantly reduce patulin levels, it often fails to fully remove heat resistant patulin-producing fungi, such as B. nivea and B. fulva [232], allowing for potential continued production of patulin within the finished juice.

3.3. Filtering and adsorption

A number of studies have been devoted to the removal of patulin from juice through the use of adsorption filters, columns, and agitation treatments using carbon-based material. Agitation with 20 mg/mL activated charcoal followed by filtration through a 40-or 60-mesh charcoal column reduced a 30 µg/mL patulin to below detectable levels. Further, use of 5 mg/mL charcoal in agitation was able to reduce patulin to below detectable levels in naturally contaminated cider. However, colour loss was markedly present in this resulting juice [233]. In a study Kadakal and Nas [234] added different amounts of activated charcoal viz., 0, 0.5, 1.0, 1.5, 2.0, 2.5, and 3 g/L to naturally contaminated apple juice containing 62.3 ppb patulin. Samples were mixed for 0, 5, 10, 20, and 30 min. Three grams/litre activated charcoal was found to be most effective with a time period of 5 min. Clearness of juice increased, colour of juice decreased and small decreases in fumaric acid, pH and Brix (a measure of the dissolved sugar-to-water mass ratio of a solution) were also seen. In another study, ultrafine activated carbon was bound to granular quartz producing a composite carbon adsorbent (CCA). Columns with varying amounts of CCA were prepared and 10 µg/mL patulin were filtered through at 1 mL/min. Fifty percent breakthrough values for columns with 1.0, 0.5, and 0.25 g CCA were 137.5, 38.5, and 19.9 µg respectively [235]. In a study designed to compare the effects of different carbon activation methods, steam activated carbons, NORIT SA 4 and NORIT SX 4 removed 80% and 70% respectively, of an initial 1 g/L patulin solution in 12 Brix juice at 55 °C. Chemically activated carbon NORIT CA 1 removed only 45% of the same solution. This study also showed that increased Brix levels of juice led to decreased patulin removal efficiency, with NORIT SA 4 removing only 20% of patulin from 20 Brix juice. Within this study, patulin removal was independent of juice temperature between 30 and 65 °C [236]. In another study, patulin binding to activated carbon compounds was shown to be endothermic, with increased temperatures resulting in improved patulin removal [237].
Despite these initial studies where activated carbon was shown to reduce patulin, little work has been done to optimize carbon for this process. Furthermore, the use of activated carbon poses a substantial cost to the juice industry [236], being both time consuming and expensive [224]. In addition to the cost of the carbon material itself, activated charcoal treatment creates excess waste that must be dealt with ecologically [223]. Also, as mentioned within several of these studies, negative effects on colour, fumaric acid content, pH, and °Brix have been observed with carbon adsorption. These and other modifications made by carbon absorption treatments can negatively alter the taste perception and quality of the juice [237]. Finally, the use of clays can pose a risk due to removal of essential nutrients from juice [238]. Similar analysis with activated carbon compounds has not been performed, but could very likely have the same negative result.

3.4. Electromagnetic irradiation

Electromagnetic radiation has been shown in preliminary studies to reduce the content of patulin and other mycotoxins within juice. In one study, treatment of juice with 0.35-kGy ionizing radiation caused a 50% reduction of patulin with no acceleration of non-enzymatic browning of the juice [239]. The UV light sensitivity of aflatoxins has been demonstrated on several accounts, but no studies have been performed specifically on patulin [240,241].

3.5. Biological control

Biological methods of patulin control result largely from the observation that patulin is almost always completely degraded during yeast fermentation. Besides being successful, this method is much better understood compared with other decontamination methods. Approximately 96% of patulin can be removed during yeast fermentation [242]. Stinson et al. [243] examined and found that out of eight yeast strains tested six reduced patulin levels to below detectable levels, while all eight strains resulted in a 99% or better decrease in total patulin content. However, a control, stored for an equal amount of time (two weeks), had only a 10% reduction. In another study by Harwig et al. [244] observed that yeast fermentation reduced patulin levels completely after two weeks. They also noticed that patulin levels failed to decrease significantly in juices that had been yeast fermented then filter sterilized to remove yeast, suggesting that active yeast and not their by-products, were required for the reduction. Moss and Long [245] observed reduction of patulin levels during fermentative growth but not aerobic growth by three strains of Saccharomyces cerevisiae. This reduction resulted in the production of two major products: E-ascladiol, patulin’s immediate biosynthetic precursor, and its isomer Z-ascladiol. These two products were also seen in the treatment of patulin with the reducing agent sodium borohydrate. E-ascladiol is itself a mycotoxin [246], which has reduced toxicity compared with patulin and also reacts with sulfhydryl-containing compounds [247]. While effective, biological control with yeast is limited to products that can be fermented. Furthermore, yeast is itself sensitive to patulin and at concentrations greater than 200 µg/mL, yeast has been completely inhibited, preventing fermentive detoxification [248]. No research has been done to examine the potential use of other fermenting microbes, such as lactic acid bacteria, in decreasing patulin content within juices. Similar reducing enzymes and environments produced by these bacteria may very well be able to degrade patulin. Finally, no research has investigated the direct enzymatic degradation of patulin. Reducing enzymes such as those involved in yeast fermentation, as well as lactone degrading enzymes such as β -lactamase, may well be able to degrade patulin alone.

4. OTA Control Methods

4.1. OTA control in coffee

Coffee post-harvest manufacturing is carried out using two processes. The dry method consists of a natural drying stage (in the sun) or an artificial drying stage, followed by mechanical dehulling. In the wet method, cherries are pulped, and resulting beans are dried and dehulled. Pulping does not result in any significant change in the OTA content. However, there is a difference between fermentative and physical mucilage removal, the latter resulting in a substantial drop in OTA levels. After hulling, only traces of the mycotoxin are found in both cases. For the dry method, there is an increase in the OTA content, suggesting neoformation during drying. However, husk removal causes complete disappearance of the toxin. Afterwards, recontamination during the storage stage might enable toxin production [249]. Once the coffee is contaminated, the industrial decaffeination process can reduce the OTA levels by 60–90% [125]. Initial studies on the roasting process influence on OTA levels indicated a reduction of 77–87% [98], 80–90% [250], 100% [251] and 90–100% [133]. However, lower OTA (0–12%) reduction has also been observed [99]. These differences can be due to different spiking methods, selectivity and sensitivity values, initial contamination levels and roasting and drying conditions or the lack of homogeneity in toxin distribution [2,126]. More recently, Blanc et al. [120] have found a loss of 84%, van der Stegen et al. [117] of more than 69%, Pittet and Royer [252] of more than 80%, Romani et al. [253] of more than 90% and Pérez de Obanos et al. [107] of 13–93%. van der Stegen et al. [117] suggested three different explanations for this reduction: physical OTA removal with the husk, isomerisation at the C-3 position into another diastereomer and thermal degradation with possible involvement of moisture. During coffee brew manufacturing, the coffee grinding entails OTA losses of 20% [121]. Coffee steaming might also promote OTA reduction about 25% [125]. Regarding brew preparation, contradictory studies exist: Tsubouchi et al. [99], Studer-Rohr et al. [119], Stegen et al. [254] indicated that all the toxin found in the coffee bean was still present in the brew, whereas Micco et al. [133] noted that 90–100% of the OTA was absent after this stage. More recently, Perez de Obanos et al. [107] have pointed out that, depending on the brew preparation method, OTA losses vary in the range 15–50%.

4.2. OTA control in beer and wine

OTA detoxification strategies are classified depending on the type of treatment, physical, chemical or microbiological and their objective is to reduce or eliminate the OTA toxic effects by destroying, modifying or absorbing this mycotoxin [255]. The ideal detoxification method would be easy to use and economical and would not generate toxic compounds or alter other food quality parameters such as nutrient content [256]. Thus, firstly, the effect of processing stages on the toxin reduction should be studied [255]. In the case that this would not be possible, other additional treatments (physical, chemical or microbiological) should be considered.

4.3. Physical methods

In the European Union, dilution with non-contaminated foodstuffs is forbidden. Initially, it was suggested that in order to avoid OTA, the external layers in contact with the producing fungi should be eliminated [257]. However, in the case of grapes this operation is complex and has been rejected. It has also been proposed to eliminate the products colonized by fungi from the manufacturing process. However, fungus absence does not imply the mycotoxin is gone and the contamination might not be fully controlled [125]. Thermal treatments do not completely eliminate OTA [258]. A freezing (-20 °C), defrost (26 °C) process and UV and gamma treatments are able to diminish the producing fungal conidia; however, only gamma radiation can destroy the OTA [259,260,261].

4.4. Chemical methods

OTA detoxification with chemical compounds such as activated charcoal, cholestyramine, sodium and calcium aluminium silicates (mainly zeolites), bentonite, wood fragments or yeast [262,263,264,265,266,267,268,269,270,271,272] has been tested. Some of these compounds were tested in vivo; however, their activity was not as high as expected except for the activated charcoal [269]. Activated charcoal use, however, has been rejected due to the essential nutrient retention and animal poisoning [270]. Wine fining agents such as potassium caseinate or activated carbon have shown positive effects on OTA detoxification (reduction up to 82%) but they have also damaged wine quality [271,272]. A new insoluble vegetal fibre has been developed in order to adsorb the OTA present in liquid food products or, in the case of beer, present during the brewing step [273]. Currently, the most promising adsorbent materials are modified zeolites (reduction up to 72%) [274,275,276,277].

4.5. Microbiological methods

Carboxypeptidase A is an enzyme capable of destroying OTA [261] and the use of atoxigenic A. niger strains as carboxypeptidase sources has been suggested [278]. Other enzymes that can be obtained from A. niger strains and can efficiently degrade OTA are lipases [279], an crude enzyme preparation [280] and a metalloenzyme [281]. A carboxypeptidase present in Phaffia rhodozyma can also degrade OTA up to 90% [264]. Moreover, certain bacteria belonging to Streptococcus, Bifidobacterium, Lactobacillus, Butyribrio, Phenylobacterium, Pleurotus, Saccharomyces, Bacillus and Acinetobacter genera [282] and certain fungi belonging to Aspergillus (A. fumigatus, A. niger, A. carbonarius, A. japonicus, A. versicolor, A. wentii and A. ochraceus), Alternaria, Botrytis, Cladosporium, Phaffia, Penicillum and Rhizopus (R. stolonifer and R. oryzae) genera [264,271,283], are able to degrade OTA in vitro up to more than 95%. Moreover, some of them have shown detoxifying properties in in vivo assays [282].
Microbiological control of OTA is also important during wine manufacturing. OTA content increases until the malolactic fermentation step preceding bottling of wine. During this time the mycotoxin level diminishes, probably due to its adsorption on the Saccharomyces yeast surface, to its interaction with metabolites produced by yeast or its degradation by the lactic bacteria still present in wine [284,285,286]. Grape washing, cooking and pressing can also promote the OTA content drop [53,287]. During vine fruits production, efficient drying and turning over are required because in these stages, moisture and sugar content stimulates Aspergillus section Nigri development and OTA synthesis [288].

5. Conclusions

While studies investigating the health effects of patulin and OTA have proved inconclusive, there is little doubt as to the potential danger inherent in the contamination of juices and beverages by these two toxins. Past research has elucidated a great deal about the chemical and biological nature of patulin and many advances have been made in developing methods for detecting OTA. Research has also revealed a number of potential control measures that may provide a basis for fully effective control measures in the future. Still, patulin and OTA contamination continues to affect juices and beverages. Thus, future research must continue to address the threat of patulin and OTA contamination in juices and beverages, with an emphasis upon the following areas.
Future research on patulin control measures should be focused primarily on those steps from pre- to post-harvest that have the highest impact on reducing the probability of patulin accumulation on apples destined for juice production. Although steps such as fruit storage, fruit washing and fruit selection have a considerable impact for patulin reduction in apple juice, control should always be focused on avoiding patulin production rather than reducing patulin levels, since the large variability between conditions and techniques applied by the fruit juice industry can affect the amount of patulin reduction achieved. Beyond that, control measures should not only be focused on patulin production in apples, but also should concentrate on preventing fruit contact with soil, damage during handling and encouraging better practices and conditions for fruit washing, juice filtration and pasteurisation, as well as preventing growth of heat-resistant fungi that can produce patulin in heat processed apple juices.
Due to OTA toxicity and in order to assure human and animal health, this toxin should not be present in beverages, at least above the maximum permitted levels. Substantial efforts have been exerted to study the critical points of OTA presence in the manufacturing chain of affected commodities and, due to its environmental persistence, detoxification methods have also been investigated. Pre-harvest, during harvest and post-harvest OTA prevention strategies have been accepted as the most effective approach to manage contamination with this mycotoxin. It is not possible to entirely prevent the formation of OTA in all places. However, formation can be minimized. Fungi that produce OTA grow best under certain environmental conditions. Factors that influence production of OTA by Aspergillus and Penicillium include temperature, pH and moisture. Eliminating the conditions necessary for fungal growth helps prevent formation of OTA. Cleaning and disinfecting storage and transportation equipment to prevent cross-contamination by fungi can also help minimize OTA formation. The use of other microorganisms that can compete with OTA-producing organisms is another possible method of preventing OTA formation. However, this option carries its own, possibly negative, implications, including concerns about human allergies and food adulteration.
Various studies in these aspects emphasize the need for careful control of mycotoxins and the importance of regulation by the government of each country. Government regulators should be made responsible for auditing performance of the food system through monitoring and surveillance activities and for enforcing legal and regulatory requirements. International cooperation for mycotoxin regulation in trading products or commodities is also needed. Countries should establish quality control limits for certain commodities intended for export or import. Producer countries would be stimulated to be aware of mycotoxin contamination in their exported susceptible commodities. Low-cost technologies for assessment, prevention and control of environmental mycotoxins can be transferred from developed countries to developing ones. Finally, conferences, symposiums, trainings and workshops on current information of mycotoxins should be promoted.

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Table 1. Patulin levels reported in apple juice in different countries around the world.
Table 1. Patulin levels reported in apple juice in different countries around the world.
CountryPatulin level
(range µg/kg or litre)		Reference
Argentina17-221[71]
Australia5-646[66,67]
Austria0-50[82]
Belgium0.67-38.8[83,84]
Brazil1.01-120[72,73,75,76]
Denmark<3.2-121.8[85]
Egypt500-1,000[74]
Greece0.9-11.8[86]
Holland>25[59]
India21-845[81]
Iran10.5-285[68,69]
Italy0-150[3,43,78,79,80,188]
Japan1.0-45[77]
Portugal0-12.6[87]
Spain0-170[40,55,56,57]
South Africa5-45[41]
Saudi Arabia0.057-0.104[70]
Turkey5-732.8[64,65]
Table 3. OTA levels reported in beer in different countries around the world.
Table 3. OTA levels reported in beer in different countries around the world.
CountryType of beerOTA level
(range µg or ng/litre)		Reference
BelgiumNot specified19-198[158]
BrazilNot specified1-18[159]
CanadaNot specified0.051-1.0[160]
DenmarkNot specified10-26[161]
GermanyNot specified0.01-0.29[162]
JapanNot specified<13.0[163]
IranNon-alcoholic beer60.71-96.04[164]
SpainNot specified2.4-2.5[165]
Table 4. OTA levels reported in wine in different countries around the world.
Table 4. OTA levels reported in wine in different countries around the world.
CountryType of wineOTA level
(range µg/litre)		Reference
AustraliaRed, White<0.05-0.62[198]
AustriaRed, White<0.01-0.02[199]
GermanyWhite<0.003-0.006[200]
HungaryRed, White<0.024[201]
MoroccoWhite, Rose, Red0.028-3.24[160]
ItalyRed<0.001-3.177[202]
SpainRed, White<0.05-4.24[203]
South EuropeWhite<0.01-1.36[204]
TaiwanRed<0.2-0.5[140]

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MDPI and ACS Style

Reddy, K.R.N.; Abbas, H.K.; Abel, C.A.; Shier, W.T.; Salleh, B. RETRACTED: Mycotoxin Contamination of Beverages: Occurrence of Patulin in Apple Juice and Ochratoxin A in Coffee, Beer and Wine and Their Control Methods. Toxins 2010, 2, 229-261. https://doi.org/10.3390/toxins2020229

AMA Style

Reddy KRN, Abbas HK, Abel CA, Shier WT, Salleh B. RETRACTED: Mycotoxin Contamination of Beverages: Occurrence of Patulin in Apple Juice and Ochratoxin A in Coffee, Beer and Wine and Their Control Methods. Toxins. 2010; 2(2):229-261. https://doi.org/10.3390/toxins2020229

Chicago/Turabian Style

Reddy, Kasa R. N., Hamed K. Abbas, Craig A. Abel, Wayne Thomas Shier, and Baharuddin Salleh. 2010. "RETRACTED: Mycotoxin Contamination of Beverages: Occurrence of Patulin in Apple Juice and Ochratoxin A in Coffee, Beer and Wine and Their Control Methods" Toxins 2, no. 2: 229-261. https://doi.org/10.3390/toxins2020229

APA Style

Reddy, K. R. N., Abbas, H. K., Abel, C. A., Shier, W. T., & Salleh, B. (2010). RETRACTED: Mycotoxin Contamination of Beverages: Occurrence of Patulin in Apple Juice and Ochratoxin A in Coffee, Beer and Wine and Their Control Methods. Toxins, 2(2), 229-261. https://doi.org/10.3390/toxins2020229

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