Processing Properties and Potency of Bacillus thuringiensis Cry Toxins in the Rice Leaffolder Cnaphalocrocis medinalis (Guenée)
Abstract
:1. Introduction
2. Results
2.1. Processing of Cry Toxins with Different Concentrations of C. medinalis Midgut Extracts
2.2. Processing of Cry Toxins with C. medinalis Midgut Extracts over Time
2.3. Potency of Cry Toxin Processed by C. medinalis Midgut Extracts
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Insects
5.2. Toxins
5.3. Preparation of Midgut Extracts
5.4. Processing of Cry Toxins with Different Concentrations of C. medinalis Midgut Extracts
5.5. Processing of Cry Toxins with C. medinalis Midgut Extracts over Time
5.6. Bioassays
5.7. Data analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Toxins | n | Slope (SE) | LC50 (95%FL) (μg/mL) |
---|---|---|---|
Cry1Aa | 300 | 1.684 (0.181) | 1.981 (1.556–2.472) |
Cry1Aa processed by midgut extracts | 300 | 1.562 (0.174) | 3.498 (2.438–5.136) |
Cry1Ac | 300 | 1.511 (0.180) | 0.673 (0.397–0.989) |
Cry1Ac processed by midgut extracts | 300 | 1.427 (0.168) | 1.068 (0.664–1.609) |
Cry1C | 300 | 1.695 (0.180) | 1.207 (0.961–1.506) |
Cry1C processed by midgut extracts | 300 | 1.498 (0.174) | 2.186 (1.463–3.534) |
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Yang, Y.; Wu, Z.; He, X.; Xu, H.; Lu, Z. Processing Properties and Potency of Bacillus thuringiensis Cry Toxins in the Rice Leaffolder Cnaphalocrocis medinalis (Guenée). Toxins 2023, 15, 275. https://doi.org/10.3390/toxins15040275
Yang Y, Wu Z, He X, Xu H, Lu Z. Processing Properties and Potency of Bacillus thuringiensis Cry Toxins in the Rice Leaffolder Cnaphalocrocis medinalis (Guenée). Toxins. 2023; 15(4):275. https://doi.org/10.3390/toxins15040275
Chicago/Turabian StyleYang, Yajun, Zhihong Wu, Xiaochan He, Hongxing Xu, and Zhongxian Lu. 2023. "Processing Properties and Potency of Bacillus thuringiensis Cry Toxins in the Rice Leaffolder Cnaphalocrocis medinalis (Guenée)" Toxins 15, no. 4: 275. https://doi.org/10.3390/toxins15040275