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Article

Snake Venomics and Antivenomics of Cape Cobra (Naja nivea) from South Africa: Insights into Venom Toxicity and Cross-Neutralization Activity

by
Choo Hock Tan
1,2,*,
Kin Ying Wong
1,2,
Li-Kun Huang
2,
Kae Yi Tan
3,
Nget Hong Tan
3 and
Wen-Guey Wu
2
1
Venom Research and Toxicology Laboratory, Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
2
Institute of Bioinformatics and Structural Biology, Department of Life Science, National Tsing Hua University, Hsinchu 300044, Taiwan
3
Protein and Interactomics Laboratory, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
*
Author to whom correspondence should be addressed.
Toxins 2022, 14(12), 860; https://doi.org/10.3390/toxins14120860
Submission received: 5 November 2022 / Revised: 24 November 2022 / Accepted: 30 November 2022 / Published: 7 December 2022
(This article belongs to the Special Issue Recent Updates in Venomics and Applications)
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Abstract

:
Naja nivea (Cape Cobra) is endemic to southern Africa. Envenoming by N. nivea is neurotoxic, resulting in fatal paralysis. Its venom composition, however, has not been studied in depth, and specific antivenoms against it remain limited in supply. Applying a protein decomplexation approach, this study unveiled the venom proteome of N. nivea from South Africa. The major components in the venom are cytotoxins/cardiotoxins (~75.6% of total venom proteins) and alpha-neurotoxins (~7.4%), which belong to the three-finger toxin family. Intriguingly, phospholipase A2 (PLA2) was undetected—this is a unique venom phenotype increasingly recognized in the African cobras of the Uraeus subgenus. The work further showed that VINS African Polyvalent Antivenom (VAPAV) exhibited cross-reactivity toward the venom and immunorecognized its toxin fractions. In mice, VAPAV was moderately efficacious in cross-neutralizing the venom lethality with a potency of 0.51 mg/mL (amount of venom completely neutralized per milliliter of antivenom). In the challenge-rescue model, VAPAV prevented death in 75% of experimentally envenomed mice, with slow recovery from neurotoxicity up to 24 h. The finding suggests the potential para-specific utility of VAPAV for N. nivea envenoming, although a higher dose or repeated administration of the antivenom may be required to fully reverse the neurotoxic effect of the venom.
Keywords:
proteomics; neurotoxic envenoming; immunoreactivity; immunorecognition; toxins; polyvalent antivenom
Key Contribution: This study characterized the venomic and antivenomic profiles of Cape Cobra (Naja nivea) from South Africa. The work further examined the venom’s immunoreactivity and the cross-neutralization efficacy of a hetero-specific African polyvalent antivenom against the venom’s lethality. The findings provide insight into the improvement of antivenom production and use in sub-Saharan Africa.

Graphical Abstract

1. Introduction

Snakebite envenoming is a priority neglected tropical disease designated by the World Health Organization [1]. Each year, it causes 81,000–138,000 deaths and three times as many chronic complications worldwide [2]. Sub-Saharan Africa is one of the most affected regions, with nearly 500,000 cases resulting in more than 30,000 deaths annually. Worse still, this is accompanied by a countless toll of permanent disabilities and amputations [3,4]. The annual burden of snakebite has been recently estimated at 1.03 million DALYs (disability-adjusted life years), an astonishing figure that is close to or even higher than the burden of many Neglected Tropical Diseases (NTDs) [5].
In Africa, there are approximately 400 snake species, of which 135 are medically important and capable of causing life-threatening envenoming [6]. Cobras (Naja spp.) are implicated in most bites and responsible for high fatalities. The African cobras are represented by members of three subgenera: Afronaja (African spitting cobras), Boulengerina (water cobras) and Uraeus (African non-spitting cobras) [7]. Of these, Cape cobra (Naja nivea), along with species within the Naja haje (Egyptian cobra) complex (now constituting N. haje, Naja senegalensis, Naja arabica, Naja annulifera and Naja anchietae), are grouped under the subgenus of Uraeus [7]. Cape cobra diverged from the Naja haje complex earlier, presumably following allopatric speciation, and has since remained endemic to the southern part of the African continent. On the other hand, the N. haje complex species are more widely distributed in the north of the continent. N. nivea is considered a deadly snake of high medical importance (WHO Category 1) due to its common occurrence and its bite being associated with high mortality in South Africa, South Botswana, and Namibia [8,9].
Earlier studies from the 1950s to the 1980s showed that N. nivea venom contained neurotoxins and cardiotoxins, and exhibited neurotoxic and cardiotoxic activities in animals [10,11,12,13]. However, a comprehensive venom analysis, including proteomics of N. nivea venom, remains unavailable, hindering a deeper understanding of the pathophysiology of envenoming by this species. Clinically, it has been reported that envenoming by N. nivea led to severe neurotoxicity, where complete flaccid paralysis occurred within 3 h following a bite, and the victim required mechanical ventilation for a prolonged duration of 4–7 days [14]. The cases were treated with an African antivenom (SAIMR Polyvalent Antivenom) which was raised against 10 species of viperid and elapid snakes, including N. nivea). It was reported that the antivenom was unable to fully reverse the established neurotoxicity once the paralysis set in, suggesting low effectiveness of antivenom treatment in this case. More recent research has also concluded that various antivenom products marketed in sub-Saharan Africa indeed lack efficacy or that evidence is scarce to support their clinical use [15]. The South African Institute for Medical Research (SAIMR) Polyvalent Antivenom is commonly used to treat envenoming caused by N. nivea in South Africa, but its neutralization efficacy has just been shown to be low in another recent preclinical study [16]. The situation is further exacerbated by a shortage of antivenom supply in Africa, ostensibly due to resource scarcity and low profitability of the antivenom industry. To meet the market need, foreign manufacturers, especially those based in India, have been producing antivenoms for use in Africa, with products such as VINS African Polyvalent Antivenom (VAPAV). VAPAV is raised against 10 common African venomous snake species, i.e., Naja melanoleuca, Naja nigricollis, N. haje, Dendroaspis polylepis, Dendroaspis viridis, Dendroaspis jamesoni, Bitis gabonica, Bitis arietans, Echis leucogaster, and Echis ocellatus. Of these, N. haje is phylogenetically closely related to N. nivea, and therefore VAPAV is hypothetically able to cross-neutralize the venom of N. nivea. The para-specific utility of VAPAV, however, has not been investigated and verified. Hence, this study first set to unravel the composition of N. nivea venom through a decomplexation proteomic approach. Subsequently, the study examined the immunoreactivity of VAPAV toward the venom and its toxin fractions, and scrutinized the in vivo efficacy of the antivenom in cross-neutralizing the venom’s lethality.

2. Results

2.1. Decomplexation of N. nivea Venom by Reverse-Phase HPLC

The N. nivea venom was resolved by C18 reverse-phase HPLC, yielding 17 protein fractions, as shown in Figure 1A. Electrophoretic profiles showed fractions 1–12 (eluted between 55–130 min of HPLC) contained mainly low molecular weight proteins (7–16 kDa), which accounted for nearly 87% of total venom proteins (Figure 1B). Fractions 13–17 were minor proteins eluted in the later course of fractionation (beyond 130 min of HPLC), consisting of moderate to high molecular weight proteins (>20 kDa) in the venom.

2.2. Naja nivea Venom Proteome

The tandem mass spectrometry analysis identified a total of 58 proteoforms from all fractions of N. nivea venom (Table 1). Of these, 43 proteoforms were annotated based on peptide sequences that belonged to cobras (Naja spp.; Table 2; Supplementary File S1). These proteins were sorted and categorized into 11 protein families, along with their relative protein abundances (Figure 2). Three-finger toxins (3FTx) constitute the main protein family, accounting for 84.62% of total venom proteins in which cytotoxins/cardiotoxins (CTX, 75.65%) and α-neurotoxins (short and long neurotoxins, 7.39%) are abundantly expressed. Other proteins with lower abundances (collectively < 20% of total venom proteins) belong to the toxin families of snake venom metalloproteinase (SVMP), cysteine-rich secretory protein (CRiSP), Kunitz-type serine protease inhibitor (KSPI), nerve growth factor (NGF), phosphodiesterase (PDE), 5′-nucleotidase (5′-NUC), L-amino acid oxidase (LAAO), cobra venom factor (CVF), acetylcholinesterase (AChE) and vespryn (VES; Table 2). Mass spectrometric data, including parameters for spectral ions, protein scores and the amino acid sequences of peptides, are provided in the Supplementary Materials (Supplementary File S1).

2.3. Immunological Binding Activity of VAPAV toward N. nivea Venom

In indirect ELISA, the antivenom VAPAV exhibited concentration-dependent immunoreactivity toward the venoms of N. nivea and N. haje (positive control; Figure 3). The binding activities of VAPAV toward N. nivea and N. haje venoms were comparable, as indicated by the values of its maximal absorbance and half-maximal effective concentration (EC50) for the two venoms. Its EC50 for N. nivea venom was 4.76 ± 0.92 μg/mL, and for N. haje venom, 6.74 ± 1.26 μg/mL (p > 0.05).

2.4. Immunorecognition of N. nivea Venom Fractions by VAPAV

The RP-HPLC profiles of the whole venom, immunoretained and non-immunoretained venom proteins, are shown in Figure 4 (panels A, B, and C, respectively). The result illustrates the antivenomics for VAPAV against N. nivea venom, where the antivenom was shown to immunorecognize and bind to the 12 protein fractions of venom at different degrees of immunoreactivity. Comparing between the profiles (Figure 4A–C), proteins in RP-HPLC fractions 1, 2, and 3, as well as the tailing fraction 12, were most markedly immunocaptured by VAPAV, whereas proteins in fractions 4–8 were least immunoretained. The degrees of immunoretention of venom proteins in these fractions were tabulated as percentages in Table 3. Accordingly, the VAPAV affinity column efficiently immunorecognized venom proteins in fractions 1, 2, and 3 (68–70% immunoretention). The binding activity of VAPAV for venom proteins in fraction 12 was moderate (~58% immunoretention) but low for those in fractions 4–11 (~20–25% immunoretention). The result of immunoretention was consistent with the corresponding non-immunoretained fractions, as shown in Figure 4C, where unbound proteins were washed out from the affinity column.

2.5. Venom Lethality and Neutralization by Antivenom

The intravenous median lethal dose (i.v. LD50) of N. nivea venom was determined to be 1.11 μg/g in mice (Table 4). Considering that VAPAV exhibited high immunoreactivity toward the whole venom and was able to immunorecognize most of its protein fractions, the in vivo neutralization efficacy of the antivenom was subsequently examined in mice. In the venom-antivenom preincubation test, VAPAV was able to cross-neutralize the lethality of N. nivea venom with a neutralization potency (P) of 0.51 mg venom per ml antivenom, equivalent to a normalized potency (n-P) of 3.90 mg venom per gram of antivenom protein (Table 4). For comparison, Table 4 also included EC50 values for the neutralization of N. nivea venom by other polyvalent antivenoms, i.e., products from the South African Institute for Medical Research (SAIMR), the Bioclon Institute of Mexico (Antivipmyn Africa), and the Egyptian Organization for Biological Products and Vaccines (VACSERA) based on previous reports. In the present work, the potency values of the different antivenom products were calculated based on their reported EC50, challenge doses and antivenom protein concentrations where applicable.

2.6. Experimental Envenoming and Rescue with Antivenom

The subcutaneous LD50 of N. nivea venom was 2.42 μg/g (95% C.I. 2.10–2.79) in mice, approximately one-fold higher than the intravenous LD50 (Table 4). In the experimental envenoming model, mice injected subcutaneously with 5 × s.c. LD50 N. nivea venom began to show weakness in movement within the first 30 min of envenoming. As the neurotoxicity progressed, the mice showed hind limb paralysis, movement difficulty, and labored breathing. In the group of untreated mice (n = 8), complete paralysis and death ensued within 2 h from the time of experimental envenoming (Figure 5). In the treated group, an intravenous bolus of VAPAV at 250 μL administered upon the onset of hind limb paralysis (approximately 30 min post-envenoming) rescued most of the mice (75% survival) from the lethality of N. nivea venom (Figure 5). Mice that were dead (25%) despite antivenom treatment had a marginally delayed death (dying by approximately 3–4 h post-envenoming) in comparison to the untreated mice, which succumbed to death between 1–2 h post-envenoming. The treated mice which survived the experimental envenoming (75%) showed a gradual reversal of paralysis with full recovery (regaining the ability to move and eat freely) observed by 24 h post-envenoming.

3. Discussion

In Africa, N. nivea bites are a cause of neurotoxic envenoming [9,14]. Applying a protein decomplexation approach, the present study unveiled the first venom proteome of N. nivea, showing the venom composition is predominantly three-finger toxins comprised of α-neurotoxins (α-NTX), cytotoxins/cardiotoxins (CTX) and weak neurotoxins (WTX). The α-NTX, though constituting only ~7.4% of the total venom protein, are the principal toxins responsible for neurotoxicity in N. nivea envenoming [14]. The α-NTX of N. nivea has also been shown to be highly lethal in mice with an i.v LD50 of 0.08 μg/g [20], similar to the α-NTX of various cobra species with i.v LD50 of approximately 0.1 μg/g in mice (for instance: [21,22,23]. The high lethality of N. nivea α-NTX is primarily a sequel of the post-synaptic blockade of nicotinic acetylcholine receptors (nAChR), which disrupts the neuromuscular transmission, resulting in paralysis and respiratory failure [24]. The lethal potency of cobra venom, nonetheless, is also modulated by the amount of these neurotoxins in the venom, as it has been established that the relative abundances of α-NTX in cobra venoms are strongly correlated with the venom’s lethality [25,26]. In principle, cobra venoms with a high α-NTX abundance (>20% of total venom proteins) have low LD50s below 0.2 μg/g (thus more lethal), while those containing less α-NTX (<10%) tend to have high LD50 above 1 μg/g (thus less lethal), in mice [27,28]. African cobras such as N. nubiae, N. senegalensis, and N. melanoleuca have venoms containing 12–20% α-NTX, and moderate lethal activity with an intermediate i.v. LD50 (≤0.6 μg/g) in mice [19,29,30]. On the other hand, N. nivea (present study), as with N. nigricollis, N. katiensis, N. pallida, N. mossambica, and N. annulifera have venoms containing α-NTX below 10% of total venom proteins, and therefore higher i.v. LD50s (0.9–2.4 μg/g) in mice [30,31]. Despite the somewhat low lethal potency of venom, N. nivea envenoming causes rapid and profound neurotoxicity clinically, which could be attributed to the large amount of venom injected in a bite (considering its medium-to-large body size of up to 1.8 m in length [32]), and the venom’s low human equivalent LD50 (0.09 mg/kg) upon allometric conversion. The other toxins with known neurological effects were named weak toxins (WTX), previously shown to have weak affinity toward neuronal and muscarinic receptors and low toxicity in mice (LD50, 5–80 mg/kg) [33,34]. The low abundance of WTX in N. nivea venom indicates its ancillary role in the pathophysiology of envenoming.
Cardiotoxins, interchangeably known as cobra cytotoxins (CTXs), are the most abundant toxins identified in the N. nivea venom proteome (75% of the total venom proteins). The high expression of CTX is consistent with proteomic findings in other African cobras from various subgenera, including Afronaja [30], Boulengerina [29], and Uraeus [19,31]. Compared with the α-NTX, cobra CTXs are relatively less potent in lethal activity, with higher i.v. LD50 beyond 1 μg/g in mice [22,23,35]. CTXs are usually implicated in local tissue necrosis and venom ophthalmia due to their cytolytic effect, which can be potentiated by synergistic PLA2 in snake venom [36,37]. The cytolytic activity of CTX varies between CTX proteoforms depending on the amino acid residue present at the tip of the protein’s loop II. Based on this characteristic, CTXs are categorized as P-type (Pro30) or S-type (Ser28). Studies on CTX interaction with model lipid membranes demonstrated that the P-type CTXs are more cytolytic than the S-type [38,39], while the latter (S-type CTX) has also been shown to be cytotoxic toward human cell lines [40]. The current study identified three CTX proteoforms, in agreement with the earlier report of three N. nivea cytotoxins (designated as V˝1, V˝2 and V˝3) whose amino acid sequences have been determined and deposited in the database as CTX 1 (Uniprot KB: P01456, S-type), CTX 2 (P01463, P-type) and CTX 3 (P01458, S-type), respectively [13]. Earlier studies, however, demonstrated that N. nivea venom and its CTXs (V˝1, V˝2, and V˝3) had rather weak hemolytic activity in vitro [13,41], and local necrosis has not been reported in in vivo experiments as well as in clinical envenoming cases [14,41]. The present study re-investigated the local tissue-damaging effect of N. nivea venom by inoculating it intradermally in C57BL/6 mice (n = 4 per dose), and found no dermonecrosis even at the highest sublethal dose tested (50 µg venom per mouse; data not shown). The finding suggests a lesser role of N. nivea CTX in the pathogenesis of local tissue necrosis or that the CTXs possibly exhibit other toxic activities, such as cardiotoxicity. Cardiotoxicity is considered a rare complication of cobra bite through disrupting the calcium regulation or mitochondria fragmentation in cardiac muscle cells, resulting in cardiac arrhythmia [42,43]. Earlier studies showed that N. nivea venom caused an unusual form of cardiac failure (systolic arrest of the ventricle) ex vivo in isolated perfused mammalian heart preparation [24,41], but this toxicity has not been well documented in human envenoming cases. It is possible that the abundant CTXs interact in synergism with other toxins present in the venom, thereby contributing to the overall toxicity of the venom. Further studies are warranted to elucidate the biological activity of N. nivea CTX and its correlation with the pathophysiology of envenoming.
In addition to 3FTx, other components detected in N. nivea venom were of low abundances (3–7%). These include snake venom metalloproteinases (SVMP) and cysteine-rich secretory proteins (CRiSP). Cobra SVMPs are zinc-dependent multidomain enzymes that may be instrumental in the activation of the complement system and inflammatory response to envenoming, contributing to the venom’s toxicity [44]. CRiSP is a common component in snake venoms with diverse biological activities such as inhibition of smooth muscle contraction, blockade of various cation channels, and induction of hypothermia in prey animals [45,46]. Other minor venom components (<2%) include Kunitz-type serine protease inhibitor (KSPI), nerve growth factor (NGF), phosphodiesterase (PDE), 5′-nucleotidase (5′-NUC), L-amino acid oxidase (LAAO), cobra venom factor (CVF), acetylcholinesterase (AChE), and vespryn (VES). KSPI are small proteins (5–7 kDa) that may be involved in diverse biological activities such as blood coagulation, fibrinolysis, inflammation, and blockade of ion channels [47,48]. NGF possibly acts as an inhibitor of metalloproteinase, preventing SVMP autodigestion [49]. Snake venom PDE and 5′-NUC can act on ATP and AMP molecules to release adenosine, thus facilitating venom spread in envenomed subjects [50,51]. CVF is also known to facilitate venom spread through the activation of the complement system, thus increasing inflammation and vascular permeability [52,53]. LAAO and AChE are both large enzymatic proteins in cobra venoms. Snake venom LAAO has recently been shown to stimulate the production of inflammatory mediators, contributing to local inflammatory effects in snakebite envenoming [54]. AChE may potentiate venom-induced neurotoxicity through the degradation of the neurotransmitter acetylcholine at the neuromuscular junction [55]. VES, first discovered in King Cobra venom, has been shown to induce hyperalgesia and hypolocomotion in prey animals [56]. Although these proteins are classified as putative venom toxins, their exact roles in specific cobra envenoming await further elucidation.
Intriguingly, the present study revealed a lack of phospholipase A2 (PLA2) in the venom proteome of N. nivea. Although PLA2 has long been regarded as a ubiquitous venom component across all snake lineages, proteomic and biochemical studies increasingly showed African non-spitting cobras from the Uraeus subgenus have venoms that contain little to no PLA2 [19,31,57,58]. This is a unique venom phenotype not shared by other African and Asiatic cobras whose venoms contain a significant amount of PLA2 in general (12–20% of total venom proteins) [25,29,30,59,60]. It is speculated that the loss of PLA2 in its venom evolution partly results in the lack of cytotoxic and necrotizing properties of N. nivea venom despite containing abundant CTX, since PLA2 and cobra CTX (known as direct lytic factor previously) have been known for a long time to act in synergism [36]. Further studies are needed to elucidate the cause and mechanism (probably through pseudogenization) as well as the evolutionary impact of PLA2 loss in the venom.
In sub-Saharan Africa, resource allocation and subsequent antivenom supply, access, affordability, and availability are inadequate [61]. Domestic antivenoms are high-priced and limited in supply. Antivenoms produced outside Africa (for instance, products procured from India targeting the African snake species) are increasingly imported into Africa since their supply is consistent and the price is low. There is, however, limited data to support the clinical effectiveness of their use in Africa [15]. In this study, we scrutinized the immunoreactivity and neutralization efficacy of VAPAV, a polyvalent antivenom developed in India for use in Africa, as a para-specific antivenom against N. nivea venom. The immunological binding activity of VAPAV toward N. nivea venom was comparable to the homologous N. haje venom (the venom used in hyperimmunizing the horses), implying conserved venom protein antigenicity between the two sister species which share close phylogenetic relatedness within the subgenus of Uraeus. On antivenomics, VAPAV was also found to have considerably good immunorecognition capacity for the venom protein fractions that were eluted in the initial course of RP-HPLC (fractions 1, 2 and 3, approximately 70% of protein binding capacity). These fractions represent alpha-neurotoxins as profiled by C18 revere-phase HPLC in the proteomic study, which is also consistent with the decomplexed venom profiles of other cobra species [21,25,29,30,60], including N. annulifera and N. senegalensis from within the N. haje complex itself [19,31]. The immunoretention of these neurotoxins indicated the cross-neutralization potential of VAPAV against N. nivea venom-induced neurotoxicity. As such, the in vivo neutralization activity of VAPAV against N. nivea venom was investigated, applying the venom and antivenom preincubation protocol for immunocomplexation as per the WHO guideline [62]. Consistent with the immunoreactivity finding, VAPAV was moderately effective in cross-neutralizing the venom’s lethal effect in mice. The parameter “normalized potency” was then used to compare its neutralization efficacy with the reported efficacy of other antivenom products, i.e., SAIMR polyvalent antivenom (South African Institute for Medical Research, South Africa), Antivipmyn-Africa (Bioclon Institute, Mexico), and VACSERA (The Egyptian Company for Biological Products and Vaccines, Giza, Egypt) [16,17,18]. Of note, SAIMR polyvalent antivenom appeared to be most potent (in neutralizing N. nivea venom lethality per gram of antivenom protein), and the logical interpretation is that N. nivea venom was used as part of the immunogen cocktail for hyperimmunization during the antivenom manufacturing, in contrast to the other products that lack species-specificity for N. nivea. Between Antivipmyn and VAPAV, the former was approximately twice as potent. The immunogens used in the production of both antivenoms include cobra venoms from N. haje, N. melanoleuca and N. nigricollis, whereas Antivipmyn has an additional component from Naja pallida. Another antivenom product, VACSERA, was found to be ineffective against N. nivea venom, although N. haje and N. nigricollis venoms were included as the immunogen in its production [18]. The discrepancy in the neutralization efficacy of these antivenoms against N. nivea venom could be related to the composition of venom immunogen used by the respective manufacturers or technical factors, e.g., horses’ health conditions in mounting an immune response, immunoglobulin purification method, product purity, and experimental conditions such as the lethal dose (challenge dose) used in the neutralization assay.
Although the commonly adopted venom-antivenom preincubation technique is preferred as a standard method, it should be noted that the in vivo interaction between venom and antivenom can be complex. This can be affected by the toxicokinetics of various toxins in the venom, and the pharmacokinetics of different antivenom preparations, thus contributing to variations between studies. Thus, to further verify the in vivo cross-neutralization potential of VAPAV, the experimental envenoming and rescue mouse experiment was conducted, mimicking the actual treatment of snakebite where the antivenom is administered as indicated by the sign of neurotoxicity. The in vivo neutralization effect of VAPAV, when given as a bolus upon the onset of paralysis in the envenomed mice, was moderate. The antivenom was able to reduce fatality (rescue with VAPAV resulted in 75% survival of the envenomed mice), although the reversal of neurotoxicity was prolonged (close to 24 h) for the envenomed mice to regain normal limb functions. The finding of slow recovery from neurological paralysis echoes the clinical observation of difficulty in reversing the already developed paralysis in patients envenomed by N. nivea even with the use of the specific antivenom SAIMR [14]. In contrast, using the same experimental model of envenoming and rescue, previous works from the same laboratory showed that effective antivenoms were able to reverse venom-induced neurotoxicity rapidly, and fully rescue all mice envenomed with N. naja and N. kaouthia venoms (100% survival) [22,28]. A slow recovery from neurotoxicity indicates that a higher dose of antivenom may be required, or the antivenom may have to be administered repeatedly in order to expedite the clearance of residual toxins and promote recovery. The finding, therefore, revealed the limitation of the heterologous antivenom in cross-neutralizing N. nivea venom, and underscored the need for a poly-specific antivenom of high potency that can neutralize the venom effectively. This may be achieved by enriching the venom immunogen formulation with N. nivea venom or, more ideally, its fractionated principal neurotoxins. This can be seen in the production of an experimental pan-region, poly-specific antivenom that utilized a diverse toxin repertoire containing venoms and toxins from multiple elapid species [63,64].

4. Conclusions

The present study unveiled the venom proteome of N. nivea and showed it is dominated by three-finger toxins (3FTx, >80% of total venom proteins), especially cytotoxins/cardiotoxins (~75.6%) and alpha-neurotoxins (~7.4%). The venom proteome lacks PLA2—a unique venom phenotype increasingly recognized in the African non-spitting cobras of the Uraeus subgenus. The hetero-specific antivenom, VAPAV (an Indian antivenom product manufactured for African use) showed immunoreactivity toward the venoms of N. nivea and the phylogenetically related N. haje (whose venom was used in VAPAV production), while antivenomics indicated that the venom’s alpha-neurotoxins were immunorecognized by the antivenom with a binding capacity of ~70%. In mice, the antivenom was moderately effective in cross-neutralizing the venom’s lethality. The finding implies the need for further refinement of antivenom production, so that a pan-region antivenom with a higher potency and a broader coverage of snake species can be developed for snakebite envenoming in southern Africa where N. nivea envenoming is endemic and prevalent.

5. Materials and Methods

5.1. Chemicals and Materials

The chemicals and reagents were of analytical grade. Ammonium bicarbonate, dithiothreitol (DTT), iodoacetamide (IAA), Coomassie Brilliant Blue R-250 and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pierce™ MS grade trypsin protease, Spectra™ Multicolor Broad Range Protein Ladder (10–260 kDa, catalog number: 26634), Bicinchoninic Acid (BCA) Protein Assay kit and trifluoroacetic acid (TFA) were purchased from Thermo Scientific™ Pierce™ (Thermo Fisher Scientific, Waltham, MA, USA). Millipore ZipTip® C18 Pipette Tips and HPLC grade acetonitrile (ACN), and LiChrospher® WP 300 RP-18 (5 µm particle size) RP-HPLC column were obtained from Merck (Kenilworth, NJ, USA). Jupiter® 5μm C18 column (300 Å, LC column 250 mm × 4.6 mm) was purchased from Waters (Milford, MA, USA).

5.2. Venom and Antivenom

The venoms of N. nivea and N. haje were supplied by Latoxan (Valence, France). The antivenom used in the present study was VINS African Polyvalent Antivenom (VAPAV; product name: Snake Venom Antiserum—African; batch no.: 07AS16004; expiry date: February 2020; manufacturer: VINS Bioproducts Limited, Hyderabad, India). VAPAV is a sterile preparation containing immunoglobulin fragments F(ab)’2 derived from horses hyperimmunized with the venoms of Naja melanoleuca, Naja nigricollis, N. haje, Dendroaspis polylepis, Dendroaspis viridis, Dendroaspis jamesoni, Bitis gabonica, Bitis arietans, Echis leucogaster and Echis ocellatus. The lyophilized antivenom was reconstituted in 10 mL ultrapure water prior to use. The antivenom was used before the expiration date.

5.3. Animal Supply

ICR albino mice (20–25 g) used in the preincubation neutralization study were supplied by the Animal Experimental Unit, Faculty of Medicine, University of Malaya. Mice of the same strain and body weight were sourced from the BioLASCO Taiwan Co. Ltd. for the envenoming-rescue study conducted in the National Tsing Hua University, Taiwan. All animal study protocols were referenced to the guidelines provided by the Council for International Organizations of Medical Sciences (CIOMS) [65].

5.4. Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC)

Three milligrams of N. nivea venom were reconstituted in 200 µL ultrapure water, and the supernatant was subjected to LiChrospher® WP300 C-18 reverse-phase fractionation using Shimadzu LC-20 AD HPLC system (Shimadzu, Kyoto, Japan). The C18 column was pre-equilibrated with 0.1% TFA in water (Buffer A), and the sample was separated with 0.1% TFA in ACN (Buffer B) using a linear gradient of 5% B for 10 min, 5–15% B over 20 min, 15–45% B over 120 min and 45–70% B over 20 min at a flow rate of 1 mL/min. The elution of the proteins was monitored at wavelength 215 nm, and fractions were collected manually, lyophilized, and stored at −20 °C until use.

5.5. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)

Naja nivea venom and the RP-HPLC-collected fractions were reconstituted in ultrapure water and separated by 15% SDS-PAGE under reducing conditions at 100 V for 2 h. Spectra™ Multicolor Broad Range Protein Ladder (10–260 kDa) was used for molecular mass calibration. Gels were stained with Coomassie Brilliant Blue R-250 and scanned using Image ScannerIII Labscan 6.0 (GE Healthcare, Uppsala, Sweden).

5.6. In-Solution Tryptic Digestion and Liquid Chromatography-Tandem Mass Spectrometry

RP-HPLC-collected protein fractions (10 µg each) were reduced by dithiothreitol (DTT) and alkylated using iodoacetamide (IAA). MS-grade trypsin (Thermo Fisher Scientific, Waltham, MA, USA) was subsequently used to digest the proteins according to the manufacturer’s protocol. The peptides were then desalted and concentrated using Millipore ZipTip® C18 Pipette Tips. The digested peptides were then reconstituted in 7 µL of 0.1% formic acid in the water and analyzed using nano-electrospray ionization liquid chromatography-tandem mass spectrometry (nano-ESI-LCMS/MS). Agilent 1260 Infinity Nanoflow LC system, coupled with Agilent 6550 Accurate-Mass Q-TOF LC/MS system (Agilent, Santa Clara, CA, USA), was used for the analysis. The samples were loaded to HPLC Large-Capacity Chip Column Zorbax 300-SB-C18 (160 nL enrichment column, 75 µm × 150 mm analytical column and 5 µm particles; Agilent part no. G4240-62010; Agilent, Santa Clara, CA, USA) for peptide separation. The injection volume was 1 μL per sample, and the flow rate was set to 0.4 μL/min, with a linear gradient of 5–70% of elution solvent (0.1% formic acid in 100% acetonitrile). The flow of drying gas was delivered at 11 L/min at 290 °C. The fragmentor voltage was set to 175 V, and the capillary voltage was 1800 V. The mass spectra were obtained using Mass Hunter acquisition software (Agilent, Santa Clara, CA, USA) in MS/MS mode with an MS scan range of 200–3000 m/z and MS/MS scan range of 50–3200 m/z. Data were extracted based on MH+ mass range between 50 and 3200 Da and processed with Agilent Spectrum Mill MS Proteomics Workbench software packages version B.04.00. The merged database incorporating non-redundant NCBI databases of Serpentes (taxid: 8570) and an in-house transcripts database was used for peptide matching and protein identification purposes. Carbamidomethylation was specified as a fixed modification and oxidized methionine as a variable modification. The following filters were used for the validation of peptides and proteins: protein score >20 and peptide score >10. Results of protein identification based on ≥2 “distinct peptides” were considered significant.

5.7. Estimation of Protein Relative Abundance

The relative abundance of proteins (%) was estimated based on the chromatographic peak area of protein eluted and the mean spectral intensity (MSI) of peptides previously described [25]. The following calculation was adopted:
Relative   abundance   of   protein   A   in   fraction   Z   ( % )                                         = Mean   spectral   intensity   of   protein   A   in   fraction   Z Total   mean   spectral   intensity   in   HPLC   fraction   Z                                       ×   %   AUC   of   HPLC   fraction   Z

5.8. Immunoreactivity of Antivenom

The immunoreactivity of VAPAV toward the venom antigens of N. nivea and N. haje were studied with an indirect enzyme-linked immunosorbent assay (indirect ELISA) as previously described [66]. The wells of the 96-well immunoplate were pre-coated with 10 ng of venom antigen at 4 °C overnight. The immunoplate was flicked dry and rinsed with phosphate-buffered saline with 0.5% Tween® 20 (PBST) 4 times to remove the excess antigens. The antivenom was appropriately diluted at 1:100, 1:300, 1:900, 1:2700, 1:8100, and 1:24,300 from a stock concentration of 20 mg/mL. A total of 100 µL of diluted antivenom was then added to each of the antigen-coated wells for incubation at room temperature for 1 h. The incubates were then removed by flicking the immunoplates and washing them with PBST. 100 µL of horseradish peroxidase-conjugated antihorse-IgG (Jackson ImmunoResearch Inc., West Grove, PA, USA) pre-diluted in PBST (1:10,000) was then added into each well, and the incubation took place for 1 h at room temperature. The excess unbound conjugated antibodies were then removed by flicking the immunoplates and washing them 4 times with PBST. Fifty microliters of freshly prepared 3,3′5,5′-tetramethylbenzidine (TMB) substrate solution was subsequently added, and the enzymatic reaction took place in the dark for 10 min at room temperature. The reaction was terminated by adding 50 µL of 12.5% sulfuric acid, and the absorbance was measured at 450 nm using a Tecan i-control™ infinite M1000Pro microplate reader (Tecan, Männedorf, Switzerland). All values were means ± S.E.M. of triplicate experiments. The immunoreactivity was expressed as the half-maximal effective concentrations (EC50), interpreted as the antivenom concentration that results in 50% of the venom-antivenom binding reaction.

5.9. Antivenomics: Immunorecogniztion of N. nivea Venom Fractions by Antivenom

The ability of VAPAV to immunorecognize N. nivea venom fractions was examined using an affinity chromatography approach. Briefly, 1 mL of NHS-activated Sepharose 4 fast flow (GE Healthcare, Danderyd, Sweden) was packed in a column and washed with five matrix volumes (or column volume, CV) of 1 mM ice-cold HCl followed by two CV of coupling buffer (0.2 M NaHCO3, 0.5 M NaCl, pH 8.3). The matrix was then incubated with 50 mg of antivenom dissolved in 1 CV of coupling buffer at room temperature for 3 h. After the immobilization of antivenom to the column matrix, the non-reactive NHS matrix groups were blocked with 1 mM ethanolamine at room temperature for 30 min. The column matrix was repeatedly washed six times with 5 CV of low (0.1 M sodium acetate, 0.5 M NaCl, pH 4.0) and high pH buffer (0.5 M ethanolamine, 0.5 M NaCl, pH 8.3) before equilibration with 5 CV of binding buffer (PBS). One milligram of N. nivea venom dissolved in 1 mL of binding buffer was loaded to the antivenom-immobilized column matrix and incubated at room temperature for 30 min. Non-retained fractions were collected with 5 CV of PBS, and the immunocaptured venom fractions were eluted with 5 CV of elution buffer (0.1 mM glycine, pH 1.5, neutralized immediately with 1M Tris-HCl, pH 9.0). Both retained, and non-retained fractions were concentrated and subjected to reverse-phase high-performance liquid chromatography (RP-HPLC).
RP-HPLC was performed with a C18 column (300 Å, 250 mm × 4.6 mm particle size, 5 μm pore size; Phenomenex, CA Torrance) using a Shimadzu LC-20AD HPLC system (Shimadzu, Kyoto, Japan). The column was pre-equilibrated with 0.1 % TFA in ultrapure water, and eluted with a linear gradient of 0.1% TFA in acetonitrile (Buffer B) at 2% B for 5 min, 2–10% B over 2 min, 10–16% B over 6 min, 16–28% B over 2 min, 28–65% B over 37 min and 65–80% B over 5 min, with a flow rate of 0.3 mL/min. The venom fractions were monitored at a wavelength of 280 nm. The degree of VAPAV immunorecognition toward a venom fraction was estimated by the chromatographic peak area under the curve (AUC) using OriginLab 8.0 software (OriginLab Corporation, Northampton, UK). The immunorecognition was expressed as the percentage of proteins bound by the antivenom-coated affinity column (regarded as antivenom-treated venom) in comparison to that of the untreated venom profiled by the RP-HPLC under the same conditions.

5.10. Venom Lethality and Neutralization by Antivenom

A hundred microliters of the venom in various doses were injected intravenously into ICR mice through the caudal vein (20–25 g, n = 4 per dose). The survival ratio at each venom dose was recorded at 24 h post-injection. In the lethality neutralization assay, a challenge dose of the venom (5 × LD50) was pre-incubated with various dilutions of antivenom in a total volume of 200 µL at 37 °C for 30 min, followed by intravenous injection into the mice through the caudal vein (20–25 g, n = 4 per dose). The survival ratio of mice was recorded at 24 h post-injection. The venom intravenous median lethal dose (LD50), antivenom median effective dose (ED50) and the 95% confidence intervals (C.I.) were calculated by the Probit analysis method [67], using BioStat 2009 analysis software (AnalystSoft Inc., Walnut, CA, USA). The neutralizing capacity was also expressed as potency (P), defined as the amount of venom completely neutralized by one mL of antivenom (mg/mL), calculated as previously described [68,69]. For comparison purposes, the potency (P) was further divided by the antivenom protein concentration to obtain normalized potency (n-P), defined as the amount of venom neutralized by one gram of antivenom protein (mg/g).

5.11. Experimental Envenoming and Rescue Experiment

The experiment mimics the natural setting of snakebite and antivenom treatment. It was conducted in an envenoming and rescue model in mice as previously described [22,70]. The subcutaneous LD50 of N. nivea venom was first established by injecting the venom at various doses into the loose skin over the neck of the mice (20–25 g, n = 4 per dose). The number of deaths of the mice at each dose was recorded at 24 h. In the envenoming and rescue model, a challenge dose of venom (5 × s.c. LD50) dissolved in 50 µL saline was inoculated subcutaneously into the mice (20–25 g, n = 8). The mice were monitored closely for the development of neurological signs. In the rescue group, the antivenom (VAPAV) was administered as a bolus of 250 µL intravenously (via the caudal vein) upon the onset of neurotoxicity indicated by posterior limb paralysis. Mice in the control (untreated) group (20–25 g, n = 8) received 250 µL of saline instead upon the onset of neurotoxicity. The progress of neurotoxicity (deterioration or reversal) in the mice was closely monitored over 24 h. All mice were allowed free access to food and water ad libitum throughout the experiment.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/toxins14120860/s1, Supplementary File S1: Mass spectrometric data and peptide sequences of protein identified from the Naja nivea venom fractions using nano-ESI-LCMS/MS.

Author Contributions

Conceptualization, C.H.T., K.Y.T. and N.H.T.; Methodology, C.H.T. and K.Y.T.; Software, K.Y.W., L.-K.H. and K.Y.T.; Formal analysis, K.Y.W. and L.-K.H.; Investigation, C.H.T., K.Y.W. and L.-K.H.; Resources, C.H.T. and W.-G.W.; Data curation, C.H.T. and K.Y.T.; Writing—original draft, C.H.T.; Writing—review & editing, C.H.T., K.Y.W., K.Y.T., N.H.T. and W.-G.W.; Visualization, C.H.T., K.Y.W., L.-K.H. and K.Y.T.; Supervision, C.H.T., K.Y.T., N.H.T. and W.-G.W.; Project administration, C.H.T.; Funding acquisition, C.H.T., N.H.T. and W.-G.W. All authors have read and agreed to the published version of the manuscript.

Funding

The study was supported by research funding from the University of Malaya (BKS003-2020 and IF073-2021), and the Ministry of Science and Technology, Taiwan (110-2622-B-007-001-CC1 and 108-231-B-007-008).

Institutional Review Board Statement

The animal study protocols were approved by the Institutional Animal Care and Use Committee (IACUC) from the University of Malaya (approval number: 2019-220108/PHAR/R/TCH; date of approval 1 August 2019), and IACUC from the National Tsing Hua University (approval number: 110011; date of approval 1 March 2021).

Informed Consent Statement

Not applicable.

Data Availability Statement

The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the iProX partner repository (http://www.iprox.org) [71] with the dataset identifier PXD028220 (data depository date: 7 September 2021).

Acknowledgments

The authors would like to thank the University of Malaya for its research facility support.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. WHO. Guidelines for the Management of Snakebites, 2nd ed.; WHO Regional Office for Southeast Asia: Dhaka, Bangladesh, 2016. [Google Scholar]
  2. Gutiérrez, J.M.; Calvete, J.J.; Habib, A.G.; Harrison, R.A.; Williams, D.J.; Warrell, D.A. Snakebite envenoming. Nat. Rev. Dis. Prim. 2017, 3, 17063. [Google Scholar] [CrossRef] [PubMed]
  3. Chippaux, J.-P.; Massougbodji, A.; Habib, A.G. The WHO strategy for prevention and control of snakebite envenoming: A sub-Saharan Africa plan. J. Venom. Anim. Toxins Incl. Trop. Dis. 2019, 25, e20190083. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Kasturiratne, A.; Wickremasinghe, A.R.; de Silva, N.; Gunawardena, N.K.; Pathmeswaran, A.; Premaratna, R.; Savioli, L.; Lalloo, D.G.; de Silva, H.J. The global burden of snakebite: A literature analysis and modelling based on regional estimates of envenoming and deaths. PLoS Med. 2008, 5, e218. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Halilu, S.; Iliyasu, G.; Hamza, M.; Chippaux, J.-P.; Kuznik, A.; Habib, A.G. Snakebite burden in Sub-Saharan Africa: Estimates from 41 countries. Toxicon Off. J. Int. Soc. Toxinology 2019, 159, 1–4. [Google Scholar] [CrossRef]
  6. Benjamin, J.M.; Abo, B.N.; Brandehoff, N. Review Article: Snake Envenomation in Africa. Curr. Trop. Med. Rep. 2020, 7, 1–10. [Google Scholar] [CrossRef]
  7. Wallach, V.; Wüster, W.; Broadley, G.D. In Praise Of Subgenera: Taxonomic Status Of Cobras Of The Genus Naja Laurenti (Serpentes: Elapidae). Zootaxa 2009, 2236, 26–36. [Google Scholar] [CrossRef]
  8. Broadley, D.G.; Wüster, W. A review of the southern African ‘non-spitting’ cobras (Serpentes: Elapidae: Naja). Afr. J. Herpetol. 2004, 53, 101–122. [Google Scholar] [CrossRef]
  9. World Health Organization. Guidelines for the Prevention and Clinical Management of Snakebite in Africa; 9290231688; World Health Organization, Regional Office for Africa: Brazzaville, Congo, 2010. [Google Scholar]
  10. Christensen, P.A. Problems of antivenene standardization revealed by the flocculation reaction. Bull. World Health Organ 1953, 9, 353–370. [Google Scholar] [PubMed]
  11. Hokama, Y.; Iwanaga, S.; Tatsuki, T.; Suzuki, T. Snake venom proteinase inhibitors. III. Isolation of five polypeptide inhibitors from the venoms of Hemachatus haemachatus (Ringhal’s corbra) and Naja nivea (Cape cobra) and the complete amino acid sequences of two of them. J. Biochem. 1976, 79, 559–578. [Google Scholar] [CrossRef] [PubMed]
  12. Joubert, F.J.; Taljaard, N. Snake venoms. The amino acid sequences of two Melanoleuca-type toxins. Hoppe. Seylers Z. Physiol. Chem. 1980, 361, 425–436. [Google Scholar] [CrossRef] [PubMed]
  13. Botes, D.P.; Viljoen, C.C. The amino acid sequence of three non-curarimimetic toxins from Naja nivea venom. Biochim. Et Biophys. Acta (BBA)—Protein Struct. 1976, 446, 1–9. [Google Scholar] [CrossRef]
  14. Blaylock, R.S.; Lichtman, A.R.; Potgieter, P.D. Clinical manifestations of Cape cobra (Naja nivea) bites. A report of 2 cases. South Afr. Med. J. Suid-Afrik. Tydskr. Vir Geneeskd. 1985, 68, 342–344. [Google Scholar]
  15. Potet, J.; Smith, J.; McIver, L. Reviewing evidence of the clinical effectiveness of commercially available antivenoms in sub-Saharan Africa identifies the need for a multi-centre, multi-antivenom clinical trial. PLoS Negl. Trop. Dis. 2019, 13, e0007551. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Whiteley, G.; Casewell, N.R.; Pla, D.; Quesada-Bernat, S.; Logan, R.A.E.; Bolton, F.M.S.; Wagstaff, S.C.; Gutiérrez, J.M.; Calvete, J.J.; Harrison, R.A. Defining the pathogenic threat of envenoming by South African shield-nosed and coral snakes (genus Aspidelaps), and revealing the likely efficacy of available antivenom. J. Proteom. 2019, 198, 186–198. [Google Scholar] [CrossRef]
  17. Ramos-Cerrillo, B.; de Roodt, A.R.; Chippaux, J.P.; Olguín, L.; Casasola, A.; Guzmán, G.; Paniagua-Solís, J.; Alagón, A.; Stock, R.P. Characterization of a new polyvalent antivenom (Antivipmyn Africa) against African vipers and elapids. Toxicon Off. J. Int. Soc. Toxinology 2008, 52, 881–888. [Google Scholar] [CrossRef] [PubMed]
  18. Seddik, S.S.; Wanas, S.; Helmy, M.H.; Hashem, M. Cross neutralization of dangerous snake venoms from Africa and the Middle East using the VACSERA polyvalent antivenom. Egyptian Organization for Biological Products & Vaccines. J. Nat. Toxins 2002, 11, 329–335. [Google Scholar]
  19. Wong, K.Y.; Tan, K.Y.; Tan, N.H.; Tan, C.H. A Neurotoxic Snake Venom without Phospholipase A2: Proteomics and Cross-Neutralization of the Venom from Senegalese Cobra, Naja senegalensis (Subgenus: Uraeus). Toxins 2021, 13, 60. [Google Scholar] [CrossRef]
  20. Botes, D.P. Snake venom toxins. The amino acid sequences of toxins alpha and beta from Naja nivea venom and the disulfide bonds of toxin alpha. J. Biol. Chem. 1971, 246, 7383–7391. [Google Scholar] [CrossRef]
  21. Palasuberniam, P.; Chan, Y.W.; Tan, K.Y.; Tan, C.H. Snake Venom Proteomics of Samar Cobra (Naja samarensis) from the Southern Philippines: Short Alpha-Neurotoxins as the Dominant Lethal Component Weakly Cross-Neutralized by the Philippine Cobra Antivenom. Front. Pharm. 2021, 12, 727756. [Google Scholar] [CrossRef]
  22. Wong, K.Y.; Tan, C.H.; Tan, N.H. Venom and Purified Toxins of the Spectacled Cobra (Naja naja) from Pakistan: Insights into Toxicity and Antivenom Neutralization. Am. J. Trop. Med. Hyg. 2016, 94, 1392–1399. [Google Scholar] [CrossRef] [Green Version]
  23. Tan, K.Y.; Tan, C.H.; Fung, S.Y.; Tan, N.H. Neutralization of the Principal Toxins from the Venoms of Thai Naja kaouthia and Malaysian Hydrophis schistosus: Insights into Toxin-Specific Neutralization by Two Different Antivenoms. Toxins 2016, 8, 86. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Loots, J.M.; Meij, H.S.; Meyer, B.J. Effects of Naja nivea venom on nerve, cardiac and skeletal muscle activity of the frog. Br. J. Pharmacol. 1973, 47, 576–585. [Google Scholar] [CrossRef] [PubMed]
  25. Tan, C.H.; Wong, K.Y.; Chong, H.P.; Tan, N.H.; Tan, K.Y. Proteomic insights into short neurotoxin-driven, highly neurotoxic venom of Philippine cobra (Naja philippinensis) and toxicity correlation of cobra envenomation in Asia. J. Proteom. 2019, 206, 103418. [Google Scholar] [CrossRef]
  26. Wong, K.Y.; Tan, K.Y.; Tan, N.H.; Gnanathasan, C.A.; Tan, C.H. Elucidating the Venom Diversity in Sri Lankan Spectacled Cobra (Naja naja) through De Novo Venom Gland Transcriptomics, Venom Proteomics and Toxicity Neutralization. Toxins 2021, 13, 558. [Google Scholar] [CrossRef] [PubMed]
  27. Tan, C.H.; Tan, K.Y.; Wong, K.Y.; Tan, N.H.; Chong, H.P. Equatorial Spitting Cobra (Naja sumatrana) from Malaysia (Negeri Sembilan and Penang), Southern Thailand, and Sumatra: Comparative Venom Proteomics, Immunoreactivity and Cross-Neutralization by Antivenom. Toxins 2022, 14, 522. [Google Scholar] [CrossRef]
  28. Tan, K.Y.; Tan, C.H.; Sim, S.M.; Fung, S.Y.; Tan, N.H. Geographical venom variations of the Southeast Asian monocled cobra (Naja kaouthia): Venom-induced neuromuscular depression and antivenom neutralization. Comp. Biochem. Physiol. Toxicol. Pharmacol. CBP 2016, 185, 77–86. [Google Scholar] [CrossRef] [PubMed]
  29. Lauridsen, L.P.; Laustsen, A.H.; Lomonte, B.; Gutierrez, J.M. Exploring the venom of the forest cobra snake: Toxicovenomics and antivenom profiling of Naja melanoleuca. J. Proteom. 2017, 150, 98–108. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  30. Petras, D.; Sanz, L.; Segura, A.; Herrera, M.; Villalta, M.; Solano, D.; Vargas, M.; Leon, G.; Warrell, D.A.; Theakston, R.D.; et al. Snake venomics of African spitting cobras: Toxin composition and assessment of congeneric cross-reactivity of the pan-African EchiTAb-Plus-ICP antivenom by antivenomics and neutralization approaches. J. Proteome. Res. 2011, 10, 1266–1280. [Google Scholar] [CrossRef] [PubMed]
  31. Tan, K.Y.; Wong, K.Y.; Tan, N.H.; Tan, C.H. Quantitative proteomics of Naja annulifera (sub-Saharan snouted cobra) venom and neutralization activities of two antivenoms in Africa. Int. J. Biol. Macromol. 2020, 158, 605–616. [Google Scholar] [CrossRef]
  32. Marais, J. A Complete Guide to the Snakes of Southern Africa; Struik Nature: Cape Town, South Africa, 2004. [Google Scholar]
  33. Nirthanan, S.; Gopalakrishnakone, P.; Gwee, M.C.; Khoo, H.E.; Kini, R.M. Non-conventional toxins from Elapid venoms. Toxicon Off. J. Int. Soc. Toxinology 2003, 41, 397–407. [Google Scholar] [CrossRef]
  34. Lyukmanova, E.N.; Shenkarev, Z.O.; Shulepko, M.A.; Paramonov, A.S.; Chugunov, A.O.; Janickova, H.; Dolejsi, E.; Dolezal, V.; Utkin, Y.N.; Tsetlin, V.I.; et al. Structural Insight into Specificity of Interactions between Nonconventional Three-finger Weak Toxin from Naja kaouthia (WTX) and Muscarinic Acetylcholine Receptors. J. Biol. Chem. 2015, 290, 23616–23630. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Leong, P.K.; Fung, S.Y.; Tan, C.H.; Sim, S.M.; Tan, N.H. Immunological cross-reactivity and neutralization of the principal toxins of Naja sumatrana and related cobra venoms by a Thai polyvalent antivenom (Neuro Polyvalent Snake Antivenom). Acta Trop. 2015, 149, 86–93. [Google Scholar] [CrossRef] [PubMed]
  36. Condrea, E.; Devries, A.; Mager, J. Hemolysis and splitting of human erythrocyte phospholipids by snake venoms. Biochim. Et Biophys. Acta 1964, 84, 60–73. [Google Scholar] [CrossRef]
  37. Gasanov, S.E.; Alsarraj, M.A.; Gasanov, N.E.; Rael, E.D. Cobra venom cytotoxin free of phospholipase A2 and its effect on model membranes and T leukemia cells. J. Membr. Biol. 1997, 155, 133–142. [Google Scholar] [CrossRef] [PubMed]
  38. Dubovskii, P.V.; Lesovoy, D.M.; Dubinnyi, M.A.; Konshina, A.G.; Utkin, Y.N.; Efremov, R.G.; Arseniev, A.S. Interaction of three-finger toxins with phospholipid membranes: Comparison of S- and P-type cytotoxins. Biochem. J. 2005, 387, 807–815. [Google Scholar] [CrossRef]
  39. Chien, K.Y.; Chiang, C.M.; Hseu, Y.C.; Vyas, A.A.; Rule, G.S.; Wu, W. Two distinct types of cardiotoxin as revealed by the structure and activity relationship of their interaction with zwitterionic phospholipid dispersions. J. Biol. Chem. 1994, 269, 14473–14483. [Google Scholar] [CrossRef]
  40. Chong, H.P.; Tan, K.Y.; Tan, C.H. Cytotoxicity of snake venoms and cytotoxins from two Southeast Asian cobras (Naja sumatrana, Naja kaouthia): Exploration of anticancer potential, selectivity, and cell death mechanism. Front. Mol. Biosci. 2020, 7, 583587. [Google Scholar] [CrossRef]
  41. Epstein, D. The pharmacology of the venom of the cape cobra. Q. J. Exp. Physiol. 1930, 20, 7–19. [Google Scholar] [CrossRef] [Green Version]
  42. Sudulagunta, S.R.; Sodalagunta, M.B.; Khorram, H.; Sepehrar, M.; Noroozpour, Z. Case Report Cardiotoxicity and respiratory failure due to Cobra bite. Sch. J. Appl. Med. Sci. 2015, 3, 1830–1833. [Google Scholar]
  43. Senthilkumaran, S.; Meenakshisundaram, R.; Thirumalaikolundusubramanian, P.; Menezes, R.G. Cardiac toxicity following cobra envenomation. Clin. Toxicol. 2012, 50, 862, author reply 863. [Google Scholar] [CrossRef] [Green Version]
  44. Silva de França, F.; Villas-Boas, I.M.; Cogliati, B.; Woodruff, T.M.; Reis, E.d.S.; Lambris, J.D.; Tambourgi, D.V. C5a-C5aR1 axis activation drives envenomation immunopathology by the snake Naja annulifera. Front. Immunol. 2021, 12, 652242. [Google Scholar] [CrossRef] [PubMed]
  45. Yamazaki, Y.; Hyodo, F.; Morita, T. Wide distribution of cysteine-rich secretory proteins in snake venoms: Isolation and cloning of novel snake venom cysteine-rich secretory proteins. Arch. Biochem. Biophys. 2003, 412, 133–141. [Google Scholar] [CrossRef]
  46. Tadokoro, T.; Modahl, C.M.; Maenaka, K.; Aoki-Shioi, N. Cysteine-Rich Secretory Proteins (CRISPs) From Venomous Snakes: An Overview of the Functional Diversity in A Large and Underappreciated Superfamily. Toxins 2020, 12, 175. [Google Scholar] [CrossRef] [PubMed]
  47. Mukherjee, A.K.; Mackessy, S.P.; Dutta, S. Characterization of a Kunitz-type protease inhibitor peptide (Rusvikunin) purified from Daboia russelii russelii venom. Int. J. Biol. Macromol. 2014, 67, 154–162. [Google Scholar] [CrossRef] [PubMed]
  48. Earl, S.T.H.; Richards, R.; Johnson, L.A.; Flight, S.; Anderson, S.; Liao, A.; de Jersey, J.; Masci, P.P.; Lavin, M.F. Identification and characterisation of Kunitz-type plasma kallikrein inhibitors unique to Oxyuranus sp. snake venoms. Biochimie 2012, 94, 365–373. [Google Scholar] [CrossRef]
  49. Wijeyewickrema, L.C.; Gardiner, E.E.; Gladigau, E.L.; Berndt, M.C.; Andrews, R.K. Nerve growth factor inhibits metalloproteinase-disintegrins and blocks ectodomain shedding of platelet glycoprotein VI. J. Biol. Chem. 2010, 285, 11793–11799. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  50. Aird, S.D. Ophidian envenomation strategies and the role of purines. Toxicon Off. J. Int. Soc. Toxinology 2002, 40, 335–393. [Google Scholar] [CrossRef]
  51. Aird, S.D. Taxonomic distribution and quantitative analysis of free purine and pyrimidine nucleosides in snake venoms. Comp. Biochem. Physiology. Part B Biochem. Mol. Biol. 2005, 140, 109–126. [Google Scholar] [CrossRef]
  52. Vogel, C.-W.; Bredehorst, R.; Fritzinger, D.C.; Grunwald, T.; Ziegelmüller, P.; Kock, M.A. Structure and Function of Cobra Venom Factor, the Complement-Activating Protein in Cobra Venom. In Natural Toxins 2: Structure, Mechanism of Action, and Detection; Singh, B.R., Tu, A.T., Eds.; Springer: Boston, MA, USA, 1996; pp. 97–114. [Google Scholar]
  53. Vogel, C.-W.; Fritzinger, D.C. Cobra Venom Factor: The Unique Component of Cobra Venom That Activates the Complement System. In Snake Venoms; Inagaki, H., Vogel, C.-W., Mukherjee, A.K., Rahmy, T.R., Eds.; Springer: Dordrecht, The Netherlands, 2017; pp. 345–404. [Google Scholar]
  54. Paloschi, M.V.; Boeno, C.N.; Lopes, J.A.; Rego, C.M.A.; Silva, M.D.S.; Santana, H.M.; Serrath, S.N.; Ikenohuchi, Y.J.; Farias, B.J.C.; Felipin, K.P. Reactive oxygen species-dependent-NLRP3 inflammasome activation in human neutrophils induced by l-amino acid oxidase derived from Calloselasma rhodostoma venom. Life Sci. 2022, 308, 120962. [Google Scholar] [CrossRef]
  55. Cousin, X.; Bon, C. Acetylcholinesterase from snake venom as a model for its nerve and muscle counterpart. J. Nat. Toxins 1999, 8, 285–294. [Google Scholar]
  56. Pung, Y.F.; Kumar, S.V.; Rajagopalan, N.; Fry, B.G.; Kumar, P.P.; Kini, R.M. Ohanin, a novel protein from king cobra venom: Its cDNA and genomic organization. Gene 2006, 371, 246–256. [Google Scholar] [CrossRef] [PubMed]
  57. Tan, C.H.; Wong, K.Y.; Tan, N.H.; Ng, T.S.; Tan, K.Y. Distinctive Distribution of Secretory Phospholipases A2 in the Venoms of Afro-Asian Cobras (Subgenus: Naja, Afronaja, Boulengerina and Uraeus). Toxins 2019, 11, 116. [Google Scholar] [CrossRef] [Green Version]
  58. Malih, I.; Rusmili, A.M.R.; Tee, T.Y.; Saile, R.; Ghalim, N.; Othman, I. Proteomic analysis of Moroccan cobra Naja haje legionis venom using tandem mass spectrometry. J. Proteom. 2014, 96, 240–252. [Google Scholar] [CrossRef] [PubMed]
  59. Tan, K.Y.; Tan, C.H.; Fung, S.Y.; Tan, N.H. Venomics, lethality and neutralization of Naja kaouthia (monocled cobra) venoms from three different geographical regions of Southeast Asia. J Proteom. 2015, 120, 105–125. [Google Scholar] [CrossRef] [PubMed]
  60. Tan, N.H.; Wong, K.Y.; Tan, C.H. Venomics of Naja sputatrix, the Javan spitting cobra: A short neurotoxin-driven venom needing improved antivenom neutralization. J. Proteom. 2017, 157, 18–32. [Google Scholar] [CrossRef]
  61. Habib, A.G.; Musa, B.M.; Iliyasu, G.; Hamza, M.; Kuznik, A.; Chippaux, J.-P. Challenges and prospects of snake antivenom supply in sub-Saharan Africa. PLoS Negl. Trop. Dis. 2020, 14, e0008374. [Google Scholar] [CrossRef]
  62. WHO. WHO Guidelines for the Production, Control and Regulation of Snake Antivenom Immunoglobulins, 2nd ed.; WHO: Geneva, Switzerland, 2016. [Google Scholar]
  63. Ratanabanangkoon, K.; Tan, K.Y.; Pruksaphon, K.; Klinpayom, C.; Gutiérrez, J.M.; Quraishi, N.H.; Tan, C.H. A pan-specific antiserum produced by a novel immunization strategy shows a high spectrum of neutralization against neurotoxic snake venoms. Sci. Rep. 2020, 10, 11261. [Google Scholar] [CrossRef]
  64. Ratanabanangkoon, K.; Tan, K.Y.; Eursakun, S.; Tan, C.H.; Simsiriwong, P.; Pamornsakda, T.; Wiriyarat, W.; Klinpayom, C.; Tan, N.H. A Simple and Novel Strategy for the Production of a Pan-specific Antiserum against Elapid Snakes of Asia. PLoS Negl. Trop. Dis. 2016, 10, e0004565. [Google Scholar] [CrossRef] [Green Version]
  65. Howard-Jones, N. A CIOMS ethical code for animal experimentation. WHO Chron. 1985, 39, 51–56. [Google Scholar]
  66. Tan, C.H.; Wong, K.Y.; Tan, K.Y.; Tan, N.H. Venom proteome of the yellow-lipped sea krait, Laticauda colubrina from Bali: Insights into subvenomic diversity, venom antigenicity and cross-neutralization by antivenom. J. Proteom. 2017, 166, 48–58. [Google Scholar] [CrossRef]
  67. Finney, D.J. Probit Analysis, 2nd ed.; Cambridge University Press: New York, NY, USA, 1952; p. 627. [Google Scholar] [CrossRef]
  68. Morais, V.; Ifran, S.; Berasain, P.; Massaldi, H. Antivenoms: Potency or median effective dose, which to use? J. Venom. Anim. Toxins Incl. Trop. Dis. 2010, 16, 191–193. [Google Scholar] [CrossRef] [Green Version]
  69. Tan, K.Y.; Tan, N.H.; Tan, C.H. Venom proteomics and antivenom neutralization for the Chinese eastern Russell’s viper, Daboia siamensis from Guangxi and Taiwan. Sci. Rep. 2018, 8, 8545. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  70. Oh, A.M.F.; Tan, C.H.; Ariaranee, G.C.; Quraishi, N.; Tan, N.H. Venomics of Bungarus caeruleus (Indian krait): Comparable venom profiles, variable immunoreactivities among specimens from Sri Lanka, India and Pakistan. J. Proteom. 2017, 164, 1–18. [Google Scholar] [CrossRef] [PubMed]
  71. Ma, J.; Chen, T.; Wu, S.; Yang, C.; Bai, M.; Shu, K.; Li, K.; Zhang, G.; Jin, Z.; He, F. iProX: An integrated proteome resource. Nucleic Acids Res. 2019, 47, D1211–D1217. [Google Scholar] [CrossRef] [PubMed]
Toxins 14 00860 g001 550
Figure 1. Chromatographic and electrophoretic profiles of Naja nivea venom. (A) Venom fractionation using C18 reverse-phase HPLC. (B) Gel electrophoresis of reverse-phase HPLC collected protein fractions (1–17) with 15% SDS-PAGE under reducing conditions. M indicates molecular markers.
Figure 1. Chromatographic and electrophoretic profiles of Naja nivea venom. (A) Venom fractionation using C18 reverse-phase HPLC. (B) Gel electrophoresis of reverse-phase HPLC collected protein fractions (1–17) with 15% SDS-PAGE under reducing conditions. M indicates molecular markers.
Toxins 14 00860 g001
Toxins 14 00860 g002 550
Figure 2. Proteome of South African N. nivea venom profiled using C18 RP-HPLC followed by liquid chromatography-tandem mass spectrometry. The 3FTx protein family consists of CTX, SNTX, LNTX and WTX. CTX is the major component (75.64% of the total venom proteins), followed by alpha-neurotoxins (both SNTX and LNTX, 7.43%). Abbreviations: 3FTx, three-finger toxins; SNTX, short neurotoxin; LNTX, long neurotoxin; WTX, weak neurotoxin; CRiSP, cysteine-rich secretory protein; SVMP, snake venom metalloproteinase; KSPI, Kunitz-type serine protease inhibitor; LAAO, L-amino acid oxidase; PDE, phosphodiesterase; AChE, acetylcholinesterase; CVF, cobra venom factor; NGF, nerve growth factor; 5′-NUC, 5′-nucleotidase; VES, vespryn; PLA2, phospholipase A2. Inset: African Cape Cobra, N. nivea.
Figure 2. Proteome of South African N. nivea venom profiled using C18 RP-HPLC followed by liquid chromatography-tandem mass spectrometry. The 3FTx protein family consists of CTX, SNTX, LNTX and WTX. CTX is the major component (75.64% of the total venom proteins), followed by alpha-neurotoxins (both SNTX and LNTX, 7.43%). Abbreviations: 3FTx, three-finger toxins; SNTX, short neurotoxin; LNTX, long neurotoxin; WTX, weak neurotoxin; CRiSP, cysteine-rich secretory protein; SVMP, snake venom metalloproteinase; KSPI, Kunitz-type serine protease inhibitor; LAAO, L-amino acid oxidase; PDE, phosphodiesterase; AChE, acetylcholinesterase; CVF, cobra venom factor; NGF, nerve growth factor; 5′-NUC, 5′-nucleotidase; VES, vespryn; PLA2, phospholipase A2. Inset: African Cape Cobra, N. nivea.
Toxins 14 00860 g002
Toxins 14 00860 g003 550
Figure 3. Immunoreactivity of VINS African Polyvalent Antivenom (VAPAV) toward the venoms of N. nivea and N. haje. N. haje venom was used as a positive control.
Figure 3. Immunoreactivity of VINS African Polyvalent Antivenom (VAPAV) toward the venoms of N. nivea and N. haje. N. haje venom was used as a positive control.
Toxins 14 00860 g003
Toxins 14 00860 g004 550
Figure 4. Immunorecognition of N. nivea venom proteins by VAPAV. Panels illustrate the reverse-phase HPLC separation of the following into fractions: (A) whole venom proteins; (B) venom proteins immunoretained by the VAPAV-immobilized affinity column; (C) venom proteins that were not bound by the immunoaffinity column.
Figure 4. Immunorecognition of N. nivea venom proteins by VAPAV. Panels illustrate the reverse-phase HPLC separation of the following into fractions: (A) whole venom proteins; (B) venom proteins immunoretained by the VAPAV-immobilized affinity column; (C) venom proteins that were not bound by the immunoaffinity column.
Toxins 14 00860 g004
Toxins 14 00860 g005 550
Figure 5. Survival plot of mice treated with VINS African Polyvalent Antivenom (VAPAV) in a challenge and rescue model of envenoming with N. nivea venom. Mice (n = 8 per group) were subcutaneously inoculated with 5 × LD50 of the venom. The rescue group received 250 µL VAPAV upon the onset of neurotoxicity at approximately 30 min.
Figure 5. Survival plot of mice treated with VINS African Polyvalent Antivenom (VAPAV) in a challenge and rescue model of envenoming with N. nivea venom. Mice (n = 8 per group) were subcutaneously inoculated with 5 × LD50 of the venom. The rescue group received 250 µL VAPAV upon the onset of neurotoxicity at approximately 30 min.
Toxins 14 00860 g005
Table
Table 1. Proteins identified from N. nivea venom fractions profiled by C18 reverse-phase high-performance liquid chromatography and nano-ESI-LCMS/MS.
Table 1. Proteins identified from N. nivea venom fractions profiled by C18 reverse-phase high-performance liquid chromatography and nano-ESI-LCMS/MS.
Protein NameDatabase Accession aSpeciesMS Search Score bR.A. (%) c
Fraction 1
Short neurotoxin 2P01422Naja annulifera38.461.58
Fraction 2
Short neurotoxin 4P01421Naja annulifera123.041.88
Long neurotoxin 1P01390Naja nivea89.870.09
Weak toxin S4C11P01400Naja melanoleuca46.320.04
Fraction 3
Long neurotoxin 1P01390Naja nivea97.382.85
Short neurotoxin 4P01421Naja annulifera53.910.07
Weak toxin CM-11P01401Naja haje haje44.620.17
Fraction 4
Long neurotoxin 1P01390Naja nivea114.030.02
Weak toxin CM-13bP01399Naja annulifera97.340.42
Short neurotoxin 4P01421Naja annulifera34.640.08
Fraction 5
Kunitz-type serine protease inhibitor 2P00986Naja nivea141.561.16
Long neurotoxin 1P01390Naja nivea114.030.34
Weak toxin CM-10P25680Naja nivea57.780.91
Fraction 6
Long neurotoxin 1P01390Naja nivea102.630.32
Kunitz-type serine protease inhibitor 2P00986Naja nivea82.240.07
Weak toxin CM-13bP01399Naja annulifera34.60.04
Fraction 7
Cytotoxin 1P01456Naja nivea210.725.63
Cytotoxin 2P01462Naja annulifera65.610.74
Long neurotoxin 1P01390Naja nivea58.240.16
Fraction 8
Cytotoxin 1P01456Naja nivea102.980.67
Cytotoxin 8P01460Naja annulifera74.541.35
Cytotoxin 2P01463Naja nivea68.30.26
Cytotoxin 3P01458Naja nivea51.410.40
Fraction 9
Cytotoxin 2P01462Naja annulifera131.234.41
Cytotoxin 7P01466Naja annulifera116.395.03
Cytotoxin 8P01460Naja annulifera99.866.97
Cytotoxin 10P01453Naja annulifera88.362.10
Cytotoxin 3P01458Naja nivea66.720.25
Cytotoxin 1P01456Naja nivea61.693.04
Venom nerve growth factor 2Q5YF89Naja sputatrix34.620.09
Fraction 10
Cytotoxin 2P01463Naja nivea161.321.91
Cytotoxin 3P01458Naja nivea164.729.68
Cytotoxin 7P01466Naja annulifera97.352.92
Cytotoxin 10P01453Naja annulifera64.962.41
Cytotoxin 8P01460Naja annulifera52.474.93
Venom nerve growth factor 2CL429.Contig1_NnSLNaja naja42.381.12
Fraction 11
Cytotoxin homologP14541Naja kaouthia98.041.78
Cytotoxin 2P01463Naja nivea72.380.43
Cytotoxin 1P01456Naja nivea42.360.07
Cytotoxin 3P01459Naja annulifera40.310.21
Cytotoxin 1P01468Naja pallida34.490.16
Venom nerve growth factor 2Q5YF89Naja sputatrix32.840.01
Fraction 12
ThaicobrinP82885Naja kaouthia89.060.04
Cytotoxin 3P01458Naja nivea87.750.03
Cytotoxin 2P01463Naja nivea77.440.05
Cytotoxin 1P01456Naja nivea74.180.02
Cytotoxin 1P01468Naja pallida61.790.03
Cytotoxin 8P01460Naja annulifera61.720.12
Cytotoxin 10P01453Naja annulifera58.410.02
Venom nerve growth factor 2Q5YF89Naja sputatrix81.11<0.01
Cytotoxin homologP14541Naja kaouthia61.230.01
Cytotoxin 11P62394Naja haje haje44.220.01
Fraction 13
Cysteine-rich venom protein natrin-2Q7ZZN8Naja atra88.140.44
Cysteine-rich venom protein latiseminQ8JI38Laticauda semifasciata41.660.16
Venom nerve growth factor 2Q5YF89Naja sputatrix72.470.01
Zinc metalloproteinase-disintegrin atraginCL626.Contig4_NsMNaja sumatrana35.120.08
Fraction 14
Natrin-1CL85.Contig1_NnSLNaja naja139.791.41
Cysteine-rich venom protein natrin-1Q7T1K6Naja atra134.041.41
Zinc metalloproteinase-disintegrin cobrinCL2966.Contig3_NnSLNaja naja67.860.85
Scutellatease-1 CL2215.Contig1_HsMEnhydrina schistosa38.330.31
Cysteine-rich secretory protein Pg-CRPF2Q6F6Cerrophidion godmani34.780.04
SVMP-Aca-4R4G2D3Acanthophis wellsi29.960.03
Fraction 15
Natrin-1CL85.Contig1_NnSLNaja naja72.730.10
Microlepidotease-1B5KFV6Oxyuranus microlepidotus52.230.15
Metalloproteinase (Type III) 1U3EPC7Micrurus fulvius41.160.15
Zinc metalloproteinase-disintegrin-like atraginCL444.Contig1_NsMNaja sumatrana53.630.03
Hemorrhagic metalloproteinase-disintegrin-like kaouthiaginP82942Naja kaouthia49.130.21
SVMP-Aca-4R4G2D3Acanthophis wellsi44.770.01
Zinc metalloproteinase-disintegrin-like atrase-AD5LMJ3Naja atra48.071.49
Zinc metalloproteinase-disintegrin cobrinCL2966.Contig3_NnSLNaja naja47.81.52
Zinc metalloproteinase-disintegrin cobrinCL7366.Contig1_OhMOphiophagus hannah30.860.03
Fraction 16
Phosphodiesterase 1Unigene5869_NsMNaja sumatrana167.770.15
PhosphodiesteraseA0A194ARD7Micrurus tener129.250.30
Phosphodiesterase 1CL4383.Contig2_OhMOphiophagus hannah89.990.54
Snake venom 5′-nucleotidaseCL3600.Contig1_NsM2Naja sumatrana119.170.38
Snake venom 5′-nucleotidaseCL4180.Contig1_OhMOphiophagus hannah108.510.36
Zinc metalloproteinase-disintegrin-like atraginCL444.Contig1_NsMNaja sumatrana78.80.62
Zinc metalloproteinase-disintegrin-like atraginD3TTC2Naja atra45.450.21
Zinc metalloproteinase-disintegrin atraginCL2051.Contig1_NkMNaja kaouthia29.610.33
Fraction 17
Cobra venom factorUnigene370_NsM2Naja sumatrana268.750.03
Cobra venom factorCL4560.Contig1_NsMNaja sumatrana2670.04
Cobra venom factorQ91132Naja kaouthia136.910.13
L-amino-acid oxidaseCL4047.Contig1_NsM2Naja sumatrana202.090.07
L-amino-acid oxidaseA8QL58Naja atra187.130.06
L-amino acid oxidaseA8QL51Bungarus multicinctus67.970.15
L-amino-acid oxidaseCL2322.Contig1_HhSLHypnale hypnale36.770.20
AcetylcholinesteraseCL4231.Contig1_NsMNaja sumatrana114.160.05
AcetylcholinesteraseUnigene16279_OhMOphiophagus hannah85.550.05
Snake venom 5′-nucleotidaseCL3600.Contig1_NsM2Naja sumatrana77.50.01
Snake venom metalloproteinase-disintegrin-like mocarhaginQ10749Naja mossambica65.430.24
SVMP-Hop-45R4G2Y9Hoplocephalus bungaroides36.280.38
Zinc metalloproteinase-disintegrin cobrinCL115.Contig9_NkTNaja kaouthia30.060.01
Zinc metalloproteinase-disintegrin atraginCL626.Contig4_NsM2Naja sumatrana54.020.02
Zinc metalloproteinase mocarhaginUnigene25077_NnSLNaja naja32.680.04
Zinc metalloproteinase-disintegrin-like VLAIP-AQ4VM08Macrovipera lebetina30.220.11
Phosphodiesterase 1unigene5869_NsMNaja sumatrana27.96<0.01
a Protein codes with the prefixes “CL” and “Unigene” were derived from the in-house transcriptome database. b MS search score refers to the protein score based on peptides matched to databases during the protein identification process by LCMS/MS. A score of ≥20 was set as a filter for protein identification in this study. c Protein relative abundance was determined as the percentage of total venom proteins.
Table
Table 2. Venom proteome of N. nivea according to protein families and relative abundance.
Table 2. Venom proteome of N. nivea according to protein families and relative abundance.
Protein FamilyProtein NameAccession aSpeciesR.A. (%) b
Three-finger toxins (3FTX)84.62
Short neurotoxin (SNTX) 3.61
Short neurotoxin 2P01422Naja annulifera1.58
Short neurotoxin 4P01421Naja annulifera2.03
Long neurotoxin (LNTX) 3.78
Long neurotoxin 1P01390Naja nivea3.78
Weak neurotoxin (WTX) 1.58
Weak toxin CM-13bP01399Naja annulifera0.45
Weak toxin S4C11P01400Naja melanoleuca0.04
Weak toxin CM-11P01401Naja haje haje0.17
Weak toxin CM-10P25680Naja nivea0.91
Cytotoxin/cardiotoxin (CTX) 75.65
Cytotoxin 10P01453Naja annulifera4.53
Cytotoxin 1P01456Naja nivea29.43
Cytotoxin 3P01458Naja nivea10.36
Cytotoxin 3P01459Naja annulifera0.21
Cytotoxin 8P01460Naja annulifera13.37
Cytotoxin 2P01462Naja annulifera5.15
Cytotoxin 2P01463Naja nivea2.65
Cytotoxin 7P01466Naja annulifera7.95
Cytotoxin 1P01468Naja pallida0.20
Cytotoxin homologP14541Naja kaouthia1.79
Cytotoxin 11P62394Naja haje haje<0.01
Snake venom metalloproteinase (SVMP)6.79
microlepidotease-1B5KFV6Oxyuranus microlepidotus0.15
Zinc metalloproteinase-disintegrin cobrinCL115.Contig9_NkTNaja kaouthia0.01
Zinc metalloproteinase-disintegrin atraginCL2051.Contig1_NkMNaja kaouthia0.33
Scutellatease-1CL2215.Contig1_HsMHydrophis schistosus0.30
Zinc metalloproteinase-disintegrin cobrinCL2966.Contig3_NnSLNaja naja2.37
Zinc metalloproteinase-disintegrin-like atraginCL444.Contig1_NsMNaja sumatrana0.64
Zinc metalloproteinase-disintegrin atraginCL626.Contig4_NsM2Naja sumatrana0.10
Zinc metalloproteinase-disintegrin cobrinCL7366.Contig1_OhMOphiophagus hannah0.03
Zinc metalloproteinase-disintegrin-like atraginD3TTC2Naja atra0.21
Zinc metalloproteinase-disintegrin-like atrase-AD5LMJ3Naja atra1.49
Hemorrhagic metalloproteinase-disintegrin-like kaouthiaginP82942Naja kaouthia0.21
Snake venom metalloproteinase-disintegrin-like mocarhaginQ10749Naja mossambica0.24
Zinc metalloproteinase-disintegrin-like VLAIP-AQ4VM08Macrovipera lebetina0.11
SVMP-Aca-4R4G2D3Acanthophis wellsi0.04
SVMP-Hop-45R4G2Y9Hoplocephalus bungaroides0.38
Metalloproteinase (Type III) 1U3EPC7Micrurus fulvius0.15
Zinc metalloproteinase mocarhaginunigene25077_NnSLNaja naja0.04
Cysteine-rich secretory protein (CRISP) 3.56
Natrin-1CL85.Contig1_NnSLNaja naja1.51
Cysteine-rich venom protein ophaninQ7ZT98Ophiophagus hannah0.04
Cysteine-rich venom protein natrin-1Q7T1K6Naja atra1.41
Cysteine-rich venom protein natrin-2Q7ZZN8Naja atra0.44
Cysteine-rich venom protein latiseminQ8JI38Laticauda semifasciata0.16
Kunitz-type serine protease inhibitor (KSPI) 1.24
Kunitz-type serine protease inhibitor 2P00986Naja nivea1.24
Nerve growth factor (NGF) 1.22
Venom nerve growth factor 2CL429.Contig1_NnSLNaja naja1.12
Venom nerve growth factor 2Q5YF89Naja sputatrix0.10
Phosphodiesterase (PDE) 1.00
PhosphodiesteraseA0A194ARD7Micrurus tener0.30
Phosphodiesterase 1CL4383.Contig2_OhMOphiophagus hannah0.54
Phosphodiesterase 1unigene5869_NsMNaja sumatrana0.15
5′-nucleotidase (5′-NUC) 0.75
Snake venom 5′-nucleotidaseCL3600.Contig1_NsM2Naja sumatrana0.39
Snake venom 5′-nucleotidaseCL4180.Contig1_OhMOphiophagus hannah0.36
L-amino acid oxidase (LAAO) 0.48
L-amino-acid oxidaseCL4047.Contig1_NsM2Naja sumatrana0.07
L-amino-acid oxidaseA8QL58Naja atra0.06
L-amino acid oxidaseA8QL51Bungarus multicinctus0.15
L-amino-acid oxidase-likeA0A6J1W8Y3Notechis scutatus0.20
Cobra venom factor (CVF) 0.20
Cobra venom factorunigene370_NsM2Naja sumatrana0.03
Cobra venom factorCL4560.Contig1_NsMNaja sumatrana0.04
Cobra venom factorQ91132Naja kaouthia0.13
Acetylcholinesterase (AChE) 0.10
Acetylcholinesterase NS2CL4231.Contig1_NsMNaja sumatrana0.05
Acetylcholinesteraseunigene16279_OhMOphiophagus hannah0.05
Vespryn (VES) 0.04
ThaicobrinP82885Naja kaouthia0.04
a Accession codes with the suffix “_xxxx” were proteins identified based on tryptic peptides matched to sequences from an in-house transcript database. b RA: Relative abundance is represented as the percentage of total venom proteins.
Table
Table 3. Immunorecognition of N. nivea venom fractions by VAPAV.
Table 3. Immunorecognition of N. nivea venom fractions by VAPAV.
Number of Protein Fractions aImmunorecognition Capacity of VAPAV b (%)
167.0
2, 370.2
424.8
522.3
622.9
725.5
824.4
9, 10, 1131.3
1258.8
a Numbering based on the reverse-phase HPLC profiles of N. nivea venom (see Figure 4). b Percentage indicates the ratio of proteins in each fraction bound or immunoretained by the VAPAV-immobilized affinity column.
Table
Table 4. Lethality of N. nivea venom and its neutralization by antivenoms used in Africa.
Table 4. Lethality of N. nivea venom and its neutralization by antivenoms used in Africa.
i.v. LD50 a (µg/g)s.c. LD50 a (µg/g)AntivenomAntivenom Protein Concentration (mg/mL)Challenge DoseED50 b (µL)ER50 c (mg/mL)Potency d (mg/mL)Normalized Potency e (n-P)Reference
1.11
(0.73–1.69)		2.42
(2.10–2.79)		VAPAV130.68 ± 2.83 #2.574.610.86
(0.61–1.20)		0.513.90Current study
0.84
(0.57–1.04)		-SAIMR118.42.017.811.70
(1.49–1.94)		0.947.94 [16]
0.45
(0.43–0.47)		-Antivipmyn-Africa323.057.110.47
(0.47–0.48)		0.319.69 [17]
3.55 (95% CI: NA)-VACSERANANANot effective [18]
Abbreviation: i.v., intravenous; s.c., subcutaneous; LD50, median lethal dose; ED50, median effective dose; ER50, median effective ratio; NA, not available. a LD50, the dose of venom (μg) per gram of mouse body weight at which 50% of mice died. b ED50, the dose of antivenom (μL) at which 50% of mice survived. c ER50, the ratio of venom (mg) to the volume of antivenom (ml) at which 50% of mice survived. d Potency, the amount of venom (mg) completely neutralized per ml antivenom (mg/mL). e Normalized potency, the amount of venom (mg) completely neutralized per g antivenom protein (mg/g). # Protein concentration of VAPAV is as previously reported for the same sample [19].
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Tan, C.H.; Wong, K.Y.; Huang, L.-K.; Tan, K.Y.; Tan, N.H.; Wu, W.-G. Snake Venomics and Antivenomics of Cape Cobra (Naja nivea) from South Africa: Insights into Venom Toxicity and Cross-Neutralization Activity. Toxins 2022, 14, 860. https://doi.org/10.3390/toxins14120860

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Tan CH, Wong KY, Huang L-K, Tan KY, Tan NH, Wu W-G. Snake Venomics and Antivenomics of Cape Cobra (Naja nivea) from South Africa: Insights into Venom Toxicity and Cross-Neutralization Activity. Toxins. 2022; 14(12):860. https://doi.org/10.3390/toxins14120860

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Tan, Choo Hock, Kin Ying Wong, Li-Kun Huang, Kae Yi Tan, Nget Hong Tan, and Wen-Guey Wu. 2022. "Snake Venomics and Antivenomics of Cape Cobra (Naja nivea) from South Africa: Insights into Venom Toxicity and Cross-Neutralization Activity" Toxins 14, no. 12: 860. https://doi.org/10.3390/toxins14120860

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