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Article

Removal of Aflatoxin B1 by Edible Mushroom-Forming Fungi and Its Mechanism

1
Department of Integrated Biomedical and Life Science, Korea University, Seoul 02841, Korea
2
Department of Food and Nutrition, Hanyang University, Seoul 04763, Korea
*
Author to whom correspondence should be addressed.
Equal contribution.
Toxins 2021, 13(9), 668; https://doi.org/10.3390/toxins13090668
Received: 30 August 2021 / Revised: 15 September 2021 / Accepted: 16 September 2021 / Published: 18 September 2021
(This article belongs to the Special Issue Determination and Detoxification Strategies of Mycotoxins)
Aflatoxins (AFs) are biologically active toxic metabolites, which are produced by certain toxigenic Aspergillus sp. on agricultural crops. In this study, five edible mushroom-forming fungi were analyzed using high-performance liquid chromatography fluorescence detector (HPLC-FLD) for their ability to remove aflatoxin B1 (AFB1), one of the most potent naturally occurring carcinogens known. Bjerkandera adusta and Auricularia auricular-judae showed the most significant AFB1 removal activities (96.3% and 100%, respectively) among five strains after 14-day incubation. The cell lysate from B. adusta exhibited higher AFB1 removal activity (35%) than the cell-free supernatant (13%) after 1-day incubation and the highest removal activity (80%) after 5-day incubation at 40 °C. In addition, AFB1 analyses using whole cells, cell lysates, and cell debris from B. adusta showed that cell debris had the highest AFB1 removal activity at 5th day (95%). Moreover, exopolysaccharides from B. adusta showed an increasing trend (24–48%) similar to whole cells and cell lysates after 5- day incubation. Our results strongly suggest that AFB1 removal activity by whole cells was mainly due to AFB1 binding onto cell debris during early incubation and partly due to binding onto cell lysates along with exopolysaccharides after saturation of AFB1 binding process onto cell wall components. View Full-Text
Keywords: aflatoxin B1; Bjerkandera adusta; binding; mushroom; mycotoxin aflatoxin B1; Bjerkandera adusta; binding; mushroom; mycotoxin
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MDPI and ACS Style

Choo, M.-J.; Hong, S.-Y.; Chung, S.-H.; Om, A.-S. Removal of Aflatoxin B1 by Edible Mushroom-Forming Fungi and Its Mechanism. Toxins 2021, 13, 668. https://doi.org/10.3390/toxins13090668

AMA Style

Choo M-J, Hong S-Y, Chung S-H, Om A-S. Removal of Aflatoxin B1 by Edible Mushroom-Forming Fungi and Its Mechanism. Toxins. 2021; 13(9):668. https://doi.org/10.3390/toxins13090668

Chicago/Turabian Style

Choo, Min-Jung, Sung-Yong Hong, Soo-Hyun Chung, and Ae-Son Om. 2021. "Removal of Aflatoxin B1 by Edible Mushroom-Forming Fungi and Its Mechanism" Toxins 13, no. 9: 668. https://doi.org/10.3390/toxins13090668

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