Antibiofilm Activity of Acidic Phospholipase Isoform Isolated from Bothrops erythromelas Snake Venom
Abstract
:1. Introduction
2. Results
2.1. Purification and Characterization of PLA2 from B. erythromelas
2.2. Hemolytic Activity Assays
2.3. Antibacterial and Antibiofilm Activity
3. Discussion
4. Conclusions
5. Methods
5.1. B. erythromelas Venom Extraction
5.2. Quantification of Venom Proteins
5.3. Purification of Venom Proteins
5.4. Phospholipase Activity
5.5. Mass Spectrometry
5.6. Amino-Terminal Sequencing of PLA2 from B. erythromelas
5.7. Hemolysis Test
5.8. Antibacterial Activity
5.9. Antibiofilm Activity
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species | Access Number | PLA2 | Alignment | Homology (%) | Charge |
---|---|---|---|---|---|
B. erythromelas | - | PLA2 Isoform | WLVQFETLIMKIAGRSGVWYYGSYDCYCGSG | - | 0 |
B. pauloensis | D0UGJ0.1 | BpPLA2-TXI | NLVQFETLIMKIAGRSGVWYYGSYGCYCGSG | 96 | +1 |
B. diporus | AFJ79208.1 | sPLA2-II | NLVQFETLIMKIAGRSGVWYYGSYGCYCGSG | 96 | +1 |
B. erythromelas | Q2HZ28.1 | BE-I-PLA2 | SLVQFETLIMKIAGRSGVWYYGSYGCYCGSG | 96 | +1 |
***********************.****** |
Bacteria | Concentration (µM) | Ciprofloxacin (%) | Activity PLA2 (%) | IC50 PLA2 (µM) |
---|---|---|---|---|
E. coli ATCC 25922 | 37.4 | 97 ± 16 | 12 ± 20 | - |
S. aureus ATCC 7133623 | 37.4 | 90 ± 18 | 62 ± 17 | 30.2 |
A. baumannii 00332126 | 37.4 | 0 | 37 ± 10 | - |
Biofilm | ||||
A. baumannii 00332126 | 1.17 | 7 ± 6 | 53 ± 11 | 1.1 |
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Nunes, E.; Frihling, B.; Barros, E.; de Oliveira, C.; Verbisck, N.; Flores, T.; de Freitas Júnior, A.; Franco, O.; de Macedo, M.; Migliolo, L.; et al. Antibiofilm Activity of Acidic Phospholipase Isoform Isolated from Bothrops erythromelas Snake Venom. Toxins 2020, 12, 606. https://doi.org/10.3390/toxins12090606
Nunes E, Frihling B, Barros E, de Oliveira C, Verbisck N, Flores T, de Freitas Júnior A, Franco O, de Macedo M, Migliolo L, et al. Antibiofilm Activity of Acidic Phospholipase Isoform Isolated from Bothrops erythromelas Snake Venom. Toxins. 2020; 12(9):606. https://doi.org/10.3390/toxins12090606
Chicago/Turabian StyleNunes, Ellynes, Breno Frihling, Elizângela Barros, Caio de Oliveira, Newton Verbisck, Taylla Flores, Augusto de Freitas Júnior, Octávio Franco, Maria de Macedo, Ludovico Migliolo, and et al. 2020. "Antibiofilm Activity of Acidic Phospholipase Isoform Isolated from Bothrops erythromelas Snake Venom" Toxins 12, no. 9: 606. https://doi.org/10.3390/toxins12090606