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Open AccessArticle

An Integrated Proteomic and Transcriptomic Analysis Reveals the Venom Complexity of the Bullet Ant Paraponera clavata

1
School of Life Sciences, University of Technology Sydney, Broadway, NSW 2007, Australia
2
CNRS, UMR Ecologie des Forêts de Guyane, AgroParisTech, CIRAD, INRA, Université de Guyane, Université des Antilles, Campus Agronomique, BP316, CEDEX, 97379 Kourou, France
3
Institute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, Australia
4
Centre for Advanced Imaging, The University of Queensland, St. Lucia, QLD 4072, Australia
5
Brain and Mind Centre, University of Sydney, Camperdown, Sydney, NSW 2050, Australia
6
Protein Science Laboratory, Department of Biological Science, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
7
Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117600, Singapore
8
Centre for Biodiversity Dynamics, Department of Biology, Norwegian University of Science and Technology, 7491 Trondheim, Norway
9
Centre for Ecological and Evolutionary Synthesis, Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, 0316 Oslo, Norway
10
Faculty of Science, University of Nice, 06000 Nice, France
*
Author to whom correspondence should be addressed.
Toxins 2020, 12(5), 324; https://doi.org/10.3390/toxins12050324
Received: 29 March 2020 / Revised: 11 May 2020 / Accepted: 12 May 2020 / Published: 14 May 2020
(This article belongs to the Special Issue Venom Proteomics and Transcriptomics)
A critical hurdle in ant venom proteomic investigations is the lack of databases to comprehensively and specifically identify the sequence and function of venom proteins and peptides. To resolve this, we used venom gland transcriptomics to generate a sequence database that was used to assign the tandem mass spectrometry (MS) fragmentation spectra of venom peptides and proteins to specific transcripts. This was performed alongside a shotgun liquid chromatography–mass spectrometry (LC-MS/MS) analysis of the venom to confirm that these assigned transcripts were expressed as proteins. Through the combined transcriptomic and proteomic investigation of Paraponera clavata venom, we identified four times the number of proteins previously identified using 2D-PAGE alone. In addition to this, by mining the transcriptomic data, we identified several novel peptide sequences for future pharmacological investigations, some of which conform with inhibitor cysteine knot motifs. These types of peptides have the potential to be developed into pharmaceutical or bioinsecticide peptides. View Full-Text
Keywords: neurotoxins; phospholipases; hyaluronidase; RP-HPLC; DRG neurons; paraponeritoxin neurotoxins; phospholipases; hyaluronidase; RP-HPLC; DRG neurons; paraponeritoxin
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Aili, S.R.; Touchard, A.; Hayward, R.; Robinson, S.D.; Pineda, S.S.; Lalagüe, H.; Mrinalini; Vetter, I.; Undheim, E.A.B.; Kini, R.M.; Escoubas, P.; Padula, M.P.; Myers, G.S.A.; Nicholson, G.M. An Integrated Proteomic and Transcriptomic Analysis Reveals the Venom Complexity of the Bullet Ant Paraponera clavata. Toxins 2020, 12, 324.

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