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Article

A Humanized Monoclonal Antibody Cocktail to Prevent Pulmonary Ricin Intoxication

1
Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA
2
Mapp Biopharmaceutical, Inc. 6160 Lusk Blvd, San Diego, CA 92121, USA
*
Author to whom correspondence should be addressed.
Toxins 2020, 12(4), 215; https://doi.org/10.3390/toxins12040215
Received: 20 February 2020 / Revised: 24 March 2020 / Accepted: 26 March 2020 / Published: 29 March 2020
(This article belongs to the Special Issue Inhibitors and Countermeasures against Bacterial and Plant Toxins)
PB10 IgG1, a monoclonal antibody (MAb) directed against an immunodominant epitope on the enzymatic subunit (RTA) of ricin toxin (RT), has been shown to passively protect mice and non-human primates from an aerosolized lethal-dose RT challenge. However, it was recently demonstrated that the therapeutic efficacy of PB10 IgG1 is significantly improved when co-administered with a second MAb, SylH3, targeting RT’s binding subunit (RTB). Here we report that the PB10/SylH3 cocktail is also superior to PB10 alone when used as a pre-exposure prophylactic (PrEP) in a mouse model of intranasal RT challenge. The benefit of the PB10/SylH3 cocktail prompted us to engineer a humanized IgG1 version of SylH3 (huSylH3). The huPB10/huSylH3 cocktail proved highly efficacious in the mouse model, thereby opening the door to future testing in non-human primates. View Full-Text
Keywords: toxin; lung; antibody; biodefense; prophylactic toxin; lung; antibody; biodefense; prophylactic
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MDPI and ACS Style

Rong, Y.; Pauly, M.; Guthals, A.; Pham, H.; Ehrbar, D.; Zeitlin, L.; Mantis, N.J. A Humanized Monoclonal Antibody Cocktail to Prevent Pulmonary Ricin Intoxication. Toxins 2020, 12, 215. https://doi.org/10.3390/toxins12040215

AMA Style

Rong Y, Pauly M, Guthals A, Pham H, Ehrbar D, Zeitlin L, Mantis NJ. A Humanized Monoclonal Antibody Cocktail to Prevent Pulmonary Ricin Intoxication. Toxins. 2020; 12(4):215. https://doi.org/10.3390/toxins12040215

Chicago/Turabian Style

Rong, Yinghui, Michael Pauly, Adrian Guthals, Henry Pham, Dylan Ehrbar, Larry Zeitlin, and Nicholas J. Mantis. 2020. "A Humanized Monoclonal Antibody Cocktail to Prevent Pulmonary Ricin Intoxication" Toxins 12, no. 4: 215. https://doi.org/10.3390/toxins12040215

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