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Open AccessArticle

A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products

by Weiqi Zhang 1,†, Wenqin Wu 1,†, Chong Cai 1, Xiaofeng Hu 1,2,3, Hui Li 1,2,3,4,5, Yizhen Bai 1, Zhaowei Zhang 1,2,3,4,5,* and Peiwu Li 1,2,3,5
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China
National Reference Laboratory for Agricultural Testing (Biotoxin), Wuhan 430062, China
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
Laboratory of Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture, Wuhan 430062, China
Author to whom correspondence should be addressed.
These authors contribute equally to this work.
Toxins 2020, 12(3), 158; (registering DOI)
Received: 7 February 2020 / Revised: 28 February 2020 / Accepted: 1 March 2020 / Published: 3 March 2020
(This article belongs to the Collection Aflatoxins)
Sensitive and point-of-care detection of small toxic molecules plays a key role in food safety. Aflatoxin, a typical small toxic molecule, can cause serious healthcare and economic issues, thereby promoting the development of sensitive and point-of-care detection. Although ELISA is one of the official detection methods, it cannot fill the gap between sensitivity and point-of-care application because it requires a large-scale microplate reader. To employ portable readers in food safety, Pt-catalysis has attracted increasing attention due to its portability and reliability. In this study, we developed a sensitive point-of-care aflatoxin detection (POCAD) method via a portable handheld barometer. We synthesized and characterized [email protected] and [email protected] conjugated with a second antibody ([email protected]). A competitive immunoassay was established based on the homemade monoclonal antibody against aflatoxins. [email protected] was used to catalyze the production of O2 from H2O2 in a sealed vessel. The pressure of O2 was then recorded by a handheld barometer. The aflatoxin concentration was inversely proportional to the pressure recorded via the barometer reading. After optimization, a limit of detection of 0.03 ng/mL and a linear range from 0.09 to 16.0 ng/mL were achieved. Recovery was recorded as 83.1%–112.0% along with satisfactory results regarding inner- and inter-assay precision (relative standard deviation, RSD < 6.4%). Little cross-reaction was observed. Additionally, the POCAD was validated by high-performance liquid chromatography (HPLC) by using peanut and corn samples. The portable POCAD exhibits strong potential for applications in the on-site detection of small toxic molecules to ensure food safety. View Full-Text
Keywords: aflatoxin; mycotoxin; [email protected]; immunoassay; handheld barometer aflatoxin; mycotoxin; [email protected]; immunoassay; handheld barometer
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MDPI and ACS Style

Zhang, W.; Wu, W.; Cai, C.; Hu, X.; Li, H.; Bai, Y.; Zhang, Z.; Li, P. A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products. Toxins 2020, 12, 158.

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