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Open AccessArticle

A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products

by Weiqi Zhang 1,†, Wenqin Wu 1,†, Chong Cai 1, Xiaofeng Hu 1,2,3, Hui Li 1,2,3,4,5, Yizhen Bai 1, Zhaowei Zhang 1,2,3,4,5,* and Peiwu Li 1,2,3,5
1
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China
3
National Reference Laboratory for Agricultural Testing (Biotoxin), Wuhan 430062, China
4
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
5
Laboratory of Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture, Wuhan 430062, China
*
Author to whom correspondence should be addressed.
These authors contribute equally to this work.
Toxins 2020, 12(3), 158; https://doi.org/10.3390/toxins12030158 (registering DOI)
Received: 7 February 2020 / Revised: 28 February 2020 / Accepted: 1 March 2020 / Published: 3 March 2020
(This article belongs to the Collection Aflatoxins)
Sensitive and point-of-care detection of small toxic molecules plays a key role in food safety. Aflatoxin, a typical small toxic molecule, can cause serious healthcare and economic issues, thereby promoting the development of sensitive and point-of-care detection. Although ELISA is one of the official detection methods, it cannot fill the gap between sensitivity and point-of-care application because it requires a large-scale microplate reader. To employ portable readers in food safety, Pt-catalysis has attracted increasing attention due to its portability and reliability. In this study, we developed a sensitive point-of-care aflatoxin detection (POCAD) method via a portable handheld barometer. We synthesized and characterized [email protected] and [email protected] conjugated with a second antibody ([email protected]). A competitive immunoassay was established based on the homemade monoclonal antibody against aflatoxins. [email protected] was used to catalyze the production of O2 from H2O2 in a sealed vessel. The pressure of O2 was then recorded by a handheld barometer. The aflatoxin concentration was inversely proportional to the pressure recorded via the barometer reading. After optimization, a limit of detection of 0.03 ng/mL and a linear range from 0.09 to 16.0 ng/mL were achieved. Recovery was recorded as 83.1%–112.0% along with satisfactory results regarding inner- and inter-assay precision (relative standard deviation, RSD < 6.4%). Little cross-reaction was observed. Additionally, the POCAD was validated by high-performance liquid chromatography (HPLC) by using peanut and corn samples. The portable POCAD exhibits strong potential for applications in the on-site detection of small toxic molecules to ensure food safety. View Full-Text
Keywords: aflatoxin; mycotoxin; [email protected]; immunoassay; handheld barometer aflatoxin; mycotoxin; [email protected]; immunoassay; handheld barometer
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MDPI and ACS Style

Zhang, W.; Wu, W.; Cai, C.; Hu, X.; Li, H.; Bai, Y.; Zhang, Z.; Li, P. A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products. Toxins 2020, 12, 158.

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