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Toxins
Volume 12
Issue 2
10.3390/toxins12020079
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Article
Peer-Review Record

Factor XII-Deficient Chicken Plasma as a Useful Target for Screening of Pro- and Anticoagulant Animal Venom Toxins

Toxins 2020, 12(2), 79; https://doi.org/10.3390/toxins12020079
by Benedito C. Prezoto 1,* and Nancy Oguiura 2
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Toxins 2020, 12(2), 79; https://doi.org/10.3390/toxins12020079
Submission received: 18 December 2019 / Revised: 14 January 2020 / Accepted: 20 January 2020 / Published: 23 January 2020
(This article belongs to the Special Issue Assessment of Animal Toxin Function with Novel Viscoelastic Methods)

Round 1

Reviewer 1 Report

Major remarks:

What could be effect of A. mellifera venom phospholipase A2 on thrombin generation using chicken plasma?

Could the authors perform turbidity assays ? And test the effect of venom on fibrin generation using chicken plasma?

What is also different between human and chicken plasma ? in term of plasmatic factors, molecules ?

It is not clear how the authors did perform clotting assays ?

The experiments should be confirmed using PRP, ie. clot retraction, fibrin formation assays

Minor remarks:

Following sentence is not clear :

Our objective was to compare the sensitivity of recalcified human and chicken 9 plasmas to bee venom phospholipase A2 (bvPLA2) anticoagulant activity, through an intrinsic 10 pathway thromboelastometry (INTEM) assay.

English of manuscript needs to be improved

Author Response

Please see attached 

Author Response File: Author Response.pdf

Reviewer 2 Report

The manuscript entitled “Prolonged recalcification time confirms factor XII-deficient chicken plasma as a useful target for studying pro- and anticoagulant animal toxins” raises an interesting topic of venom components on the clotting of vertebrate’s blood. I enjoyed reading this well written MS. The MS presents results which are clear and have the potential to add to the literature. I have only few minor comments which I believe can improve the MS.

 

Lines 10, 51, 122, 202, 213 and 215 - unify the font size

Line 10 and following – unify the abbreviation for Phospholipases A2 from Apis melifera, use everywhere bvPLA2

Line 86 – Figure 1 and following, please add “values are presented as means ±SD”

Line 125-133 – I believe this paragraph is better suited for Introduction.

Lines 157 & 160 – replace “Ca2+” with “Ca2+”

Lines 205 & 206 – as bees and scorpions are arthropods this sentence is a bit unfortunate. Please rephrase it.

Author Response

Please see attached 

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

The authors addressed the concerns of this Reviewer. 

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