Next Article in Journal
Effect of Folic Acid Supplementation and Dietary Protein Level on Growth Performance, Serum Chemistry and Immune Response in Weanling Piglets Fed Differing Concentrations of Aflatoxin
Next Article in Special Issue
Prevalence, Genotypic and Phenotypic Characterization and Antibiotic Resistance Profile of Clostridium perfringens Type A and D Isolated from Feces of Sheep (Ovis aries) and Goats (Capra hircus) in Punjab, Pakistan
Previous Article in Journal
Cross-Reactive Carbohydrate Determinant in Apis mellifera, Solenopsis invicta and Polybia paulista Venoms: Identification of Allergic Sensitization and Cross-Reactivity
Previous Article in Special Issue
Human Botulism in France, 1875–2016
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Toxins
Volume 12
Issue 10
10.3390/toxins12100650
toxins-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Molecular Characterization of Clostridium perfringens Strains Isolated in Italy

by
Katia Forti
1,2,*,
Laura Ferroni
1,
Martina Pellegrini
1,
Deborah Cruciani
1,
Antonio De Giuseppe
1,*,
Silvia Crotti
1,
Paola Papa
1,
Carmen Maresca
1,
Giulio Severi
1,
Maria Luisa Marenzoni
2 and
Monica Cagiola
1
1
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati” Via G. Salvemini 1, 06126 Perugia, Italy
2
Dipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via S. Costanzo 4, 06126 Perugia, Italy
*
Authors to whom correspondence should be addressed.
Toxins 2020, 12(10), 650; https://doi.org/10.3390/toxins12100650
Submission received: 1 August 2020 / Revised: 29 September 2020 / Accepted: 6 October 2020 / Published: 8 October 2020
(This article belongs to the Collection Clostridium difficile/Clostridium sordellii and Clostridium perfringens Toxins)
Browse Figures
Versions Notes

Abstract

Clostridium (C.) perfringens is the causative agent of several diseases and enteric infections in animals and humans. The pathogenicity of the bacterium is largely mediated by the production of a wide range of toxins. Individual C. perfringens strains produce only subsets of this toxin repertoire, which permits the classification in seven toxinotypes (A–G). In addition, a variety of minor toxins further characterizes the single strains. The aim of this work was to evaluate, using Polymerase Chain Reaction (PCR) assays, the diversity of 632 C. perfringens strains isolated in Italy over 15 years. The genotyped strains were analyzed to determine the presence of major and minor toxins (cpe, consensus, and atypical cpb2), their geographical origins, and the source of isolation (animal species or food). Our study shows that toxinotype A had the greatest representation (93%) and correlated mainly with consensus cpb2 in a variety of animal species, as well as with atypical cpb2 in the five food samples. Type D, associated with cpe and atypical cpb2 minor toxins, was identified in 3% of the cases, and type F was identified in 2.5%. Seven type C isolates (1.1%) were detected in cattle, whereas the only type B atypical cpb2 isolated in Italy was detected in a goat, and one type E cpe+atypical cpb2 was detected in a sheep. Type G was not detected.
Keywords: Clostridium perfringens; genotyping; toxinotypes; epidemiology; consensus and atypical cpb2; cpe; enterotoxin; clostridial disease; animal sources; food; vaccine Clostridium perfringens; genotyping; toxinotypes; epidemiology; consensus and atypical cpb2; cpe; enterotoxin; clostridial disease; animal sources; food; vaccine

Share and Cite

MDPI and ACS Style

Forti, K.; Ferroni, L.; Pellegrini, M.; Cruciani, D.; De Giuseppe, A.; Crotti, S.; Papa, P.; Maresca, C.; Severi, G.; Marenzoni, M.L.; et al. Molecular Characterization of Clostridium perfringens Strains Isolated in Italy. Toxins 2020, 12, 650. https://doi.org/10.3390/toxins12100650

AMA Style

Forti K, Ferroni L, Pellegrini M, Cruciani D, De Giuseppe A, Crotti S, Papa P, Maresca C, Severi G, Marenzoni ML, et al. Molecular Characterization of Clostridium perfringens Strains Isolated in Italy. Toxins. 2020; 12(10):650. https://doi.org/10.3390/toxins12100650

Chicago/Turabian Style

Forti, Katia, Laura Ferroni, Martina Pellegrini, Deborah Cruciani, Antonio De Giuseppe, Silvia Crotti, Paola Papa, Carmen Maresca, Giulio Severi, Maria Luisa Marenzoni, and et al. 2020. "Molecular Characterization of Clostridium perfringens Strains Isolated in Italy" Toxins 12, no. 10: 650. https://doi.org/10.3390/toxins12100650

APA Style

Forti, K., Ferroni, L., Pellegrini, M., Cruciani, D., De Giuseppe, A., Crotti, S., Papa, P., Maresca, C., Severi, G., Marenzoni, M. L., & Cagiola, M. (2020). Molecular Characterization of Clostridium perfringens Strains Isolated in Italy. Toxins, 12(10), 650. https://doi.org/10.3390/toxins12100650

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop