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Open AccessArticle

A Novel Magnetic Molecular Imprinted Polymer for Selective Extraction of Zearalenone from Cereal Flours before Liquid Chromatography-Tandem Mass Spectrometry Determination

1
Department of Chemistry, Università di Roma “La Sapienza”, 00185 Rome, Italy
2
CNR, NANOTEC – Campus Ecotekne, Università del Salento, 73100 Lecce, Italy
3
Department of Science and Technological Innovation, Università del Piemonte Orientale “A. Avogadro”, 15121 Alessandria, Italy
*
Authors to whom correspondence should be addressed.
Toxins 2019, 11(9), 493; https://doi.org/10.3390/toxins11090493
Received: 1 August 2019 / Revised: 22 August 2019 / Accepted: 23 August 2019 / Published: 27 August 2019
(This article belongs to the Special Issue Advanced Methods for Mycotoxins Detection)
Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin produced by various Fusarium species and commonly occurring in corn and other cereals. Even though its acute toxicity is low, still the estrogenic activity of ZEN and metabolites is a matter of concern. In this work, a new magnetic molecularly imprinted polymer (mMIP) for the selective extraction of ZEN from cereal flours is presented. The mMIP was synthesized previously using quercetin as dummy template, and here we wanted to test its applicability to complex food samples. Analyte determination was carried out by high-performance liquid chromatography coupled to tandem mass spectrometry. The selectivity of the mMIP and the main validation method parameters were assessed. In particular, even in samples as complex as cereals, matrix effect was negligible. Although the mMIP showed cross-selectivity towards both ZEN-related and quercetin-related compounds, nonetheless ZEN recovery was > 95% for the two lower spiking levels, and the quantification limit was 0.14 ng g−1, i.e., ca. 500 times lower than the maximum limit fixed for most cereals by European law. Therefore, the material, also in comparison with a commercial sorbent, appears suitable for the application in food analysis, also to isolate ZEN at trace levels. View Full-Text
Keywords: dispersive solid-phase extraction; dummy template; liquid chromatography-tandem mass spectrometry; magnetic molecularly imprinted polymers; sample preparation; zearalenone dispersive solid-phase extraction; dummy template; liquid chromatography-tandem mass spectrometry; magnetic molecularly imprinted polymers; sample preparation; zearalenone
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Cavaliere, C.; Antonelli, M.; Cerrato, A.; La Barbera, G.; Laganà, A.; Laus, M.; Piovesana, S.; Capriotti, A.L. A Novel Magnetic Molecular Imprinted Polymer for Selective Extraction of Zearalenone from Cereal Flours before Liquid Chromatography-Tandem Mass Spectrometry Determination. Toxins 2019, 11, 493.

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