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Recombinant Antibodies against Mycolactone

1
Specifica Inc., Santa Fe, NM 87505, USA
2
Université de Haute-Alsace, Université de Strasbourg, CNRS, LIMA, UMR 7042, 68000 Mulhouse, France
3
Immunobiology of Infection Unit, Institut Pasteur, INSERM U1221, 75011 Paris, France
4
New Mexico Consortium, Los Alamos, NM 87544, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Toxins 2019, 11(6), 346; https://doi.org/10.3390/toxins11060346
Received: 28 March 2019 / Revised: 10 June 2019 / Accepted: 13 June 2019 / Published: 17 June 2019
(This article belongs to the Special Issue Mycolactone: Lipid-Like Immunosuppressive Toxin of Buruli Ulcer)
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Abstract

In the past, it has proved challenging to generate antibodies against mycolactone, the primary lipidic toxin A of Mycobacterium ulcerans causing Buruli ulcer, due to its immunosuppressive properties. Here we show that in vitro display, comprising both phage and yeast display, can be used to select antibodies recognizing mycolactone from a large human naïve phage antibody library. Ten different antibodies were isolated, and hundreds more identified by next generation sequencing. These results indicate the value of in vitro display methods to generate antibodies against difficult antigenic targets such as toxins, which cannot be used for immunization unless inactivated by structural modification. The possibility to easily generate anti-mycolactone antibodies is an exciting prospect for the development of rapid and simple diagnostic/detection methods. View Full-Text
Keywords: mycolactone; Buruli ulcer; recombinant antibody; phage display; yeast display; single chain Fv mycolactone; Buruli ulcer; recombinant antibody; phage display; yeast display; single chain Fv
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Naranjo, L.; Ferrara, F.; Blanchard, N.; Demangel, C.; D’Angelo, S.; Erasmus, M.F.; Teixeira, A.A.; Bradbury, A.R. Recombinant Antibodies against Mycolactone. Toxins 2019, 11, 346.

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