Next Article in Journal
Ricin: An Ancient Story for a Timeless Plant Toxin
Next Article in Special Issue
Biomonitoring Data for Assessing Aflatoxins and Ochratoxin A Exposure by Italian Feedstuffs Workers
Previous Article in Journal
Identification of the Fungal Pathogens of Postharvest Disease on Peach Fruits and the Control Mechanisms of Bacillus subtilis JK-14
Previous Article in Special Issue
Rapid and Simple Detection of Ochratoxin A using Fluorescence Resonance Energy Transfer on Lateral Flow Immunoassay (FRET-LFI)
Open AccessArticle

A Gold Growth-Based Plasmonic ELISA for the Sensitive Detection of Fumonisin B1 in Maize

1
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
2
Jiangxi Institute of Veterinary Drug and Feedstuff Control, Nanchang 330096, China
3
Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Toxins 2019, 11(6), 323; https://doi.org/10.3390/toxins11060323
Received: 24 April 2019 / Revised: 31 May 2019 / Accepted: 4 June 2019 / Published: 5 June 2019
(This article belongs to the Special Issue Advanced Methods for Mycotoxins Detection)
In this paper, a highly sensitive plasmonic enzyme-linked immunosorbent assay (pELISA) was developed for the naked-eye detection of fumonisin B1 (FB1). Glucose oxidase (GOx) was used as an alternative to horseradish peroxidase as the carrier of the competing antigen. GOx catalyzed the oxidation of glucose to produce hydrogen peroxide, which acted as a reducing agent to reduce Au3+ to Au on the surface of gold seeds (5 nm), This reaction led to a color change in the solution from colorless to purple, which was observable to the naked eye. Various parameters that could influence the detection performance of pELISA were investigated. The developed method exhibited a considerably high sensitivity for FB1 qualitative naked-eye detection, with a visible cut-off limit of 1.25 ng/mL. Moreover, the proposed pELISA showed a good linear range of 0.31–10 ng/mL with a half maximal inhibitory concentration (IC50) of 1.86 ng/mL, which was approximately 13-fold lower than that of a horseradish peroxidase- (HRP)-based conventional ELISA. Meanwhile, the proposed method was highly specific and accurate. In summary, the new pELISA exhibited acceptable accuracy and precision for sensitive naked-eye detection of FB1 in maize samples and can be applied for the detection of other chemical contaminants. View Full-Text
Keywords: plasmonic enzyme-linked immunosorbent assay; controlled growth kinetics; glucose oxidase; naked-eye detection; fumonisins B1 plasmonic enzyme-linked immunosorbent assay; controlled growth kinetics; glucose oxidase; naked-eye detection; fumonisins B1
Show Figures

Figure 1

MDPI and ACS Style

Zhan, S.; Zheng, L.; Zhou, Y.; Wu, K.; Duan, H.; Huang, X.; Xiong, Y. A Gold Growth-Based Plasmonic ELISA for the Sensitive Detection of Fumonisin B1 in Maize. Toxins 2019, 11, 323.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop