Isolation and Characterization of Insecticidal Toxins from the Venom of the North African Scorpion, Buthacus leptochelys
Abstract
1. Introduction
2. Results and Discussion
2.1. Characterization of the B. leptochelys Venom
2.2. Purification of Insecticidal Peptides
2.3. Primary Structure of Bl-1
2.4. Sequence Comparison
3. Conclusions
4. Materials and Methods
4.1. Collection of Venom
4.2. Bioassay
4.3. Mass Spectrometric Analysis
4.4. HPLC Purification
4.5. Determination of N-terminal Sequence
4.6. Enzymatic Digestion
4.7. Homology Modeling
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Name | N-Terminal Sequence (U = Unknown) | Similar Peptides | Toxin Classification |
---|---|---|---|
Bl-1 | ARDGYISQPENCVYHCFPGS | Lqh3, Bom3 | α-like insect and mammal toxin |
Bl-2 | URDGYLVDDUNCTFFCG | Lqh2, AaH2 | α-mammal toxin |
Bl-3 | UVRDAYIADDKNCVYTCASN | OD1, Bu1 | α-insect and mammal toxin |
Bl-4 | UKNGYAVDSSGKAPECILSNYCNNECTKV | AaHIT1, LqqIT1 | β-insect toxin |
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Yoshimoto, Y.; Miyashita, M.; Abdel-Wahab, M.; Sarhan, M.; Nakagawa, Y.; Miyagawa, H. Isolation and Characterization of Insecticidal Toxins from the Venom of the North African Scorpion, Buthacus leptochelys. Toxins 2019, 11, 236. https://doi.org/10.3390/toxins11040236
Yoshimoto Y, Miyashita M, Abdel-Wahab M, Sarhan M, Nakagawa Y, Miyagawa H. Isolation and Characterization of Insecticidal Toxins from the Venom of the North African Scorpion, Buthacus leptochelys. Toxins. 2019; 11(4):236. https://doi.org/10.3390/toxins11040236
Chicago/Turabian StyleYoshimoto, Yusuke, Masahiro Miyashita, Mohammed Abdel-Wahab, Moustafa Sarhan, Yoshiaki Nakagawa, and Hisashi Miyagawa. 2019. "Isolation and Characterization of Insecticidal Toxins from the Venom of the North African Scorpion, Buthacus leptochelys" Toxins 11, no. 4: 236. https://doi.org/10.3390/toxins11040236
APA StyleYoshimoto, Y., Miyashita, M., Abdel-Wahab, M., Sarhan, M., Nakagawa, Y., & Miyagawa, H. (2019). Isolation and Characterization of Insecticidal Toxins from the Venom of the North African Scorpion, Buthacus leptochelys. Toxins, 11(4), 236. https://doi.org/10.3390/toxins11040236