The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro
Abstract
:1. Introduction
2. Results
2.1. Characterization of the CyaAIP and CyaAUBC Preparations
2.2. PLA Activity Measurements as Monitored with the PED6 Fluorescent Probe
2.3. PLA Activity Measurements as Monitored with the B3781 Fluorescent Probe
3. Discussion
4. Material and Methods
4.1. Buffers
4.2. Protein Samples
4.3. Mass Spectrometry Analysis
Sample Preparation
4.4. LC-MS/MS Analysis of the CyaA Batches
4.5. MaxQuant Analysis
4.6. Fluorescent Probes
4.7. Preparation of PED6-Containing LUV
4.8. Preparation of B3781-Containing LUV
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Voegele, A.; Sadi, M.; Raoux-Barbot, D.; Douché, T.; Matondo, M.; Ladant, D.; Chenal, A. The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro. Toxins 2019, 11, 111. https://doi.org/10.3390/toxins11020111
Voegele A, Sadi M, Raoux-Barbot D, Douché T, Matondo M, Ladant D, Chenal A. The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro. Toxins. 2019; 11(2):111. https://doi.org/10.3390/toxins11020111
Chicago/Turabian StyleVoegele, Alexis, Mirko Sadi, Dorothée Raoux-Barbot, Thibaut Douché, Mariette Matondo, Daniel Ladant, and Alexandre Chenal. 2019. "The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro" Toxins 11, no. 2: 111. https://doi.org/10.3390/toxins11020111
APA StyleVoegele, A., Sadi, M., Raoux-Barbot, D., Douché, T., Matondo, M., Ladant, D., & Chenal, A. (2019). The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro. Toxins, 11(2), 111. https://doi.org/10.3390/toxins11020111