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Toxins 2019, 11(2), 101; https://doi.org/10.3390/toxins11020101

Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry

1
Institute of Life Technologies, University of Applied Sciences, HES-SO Valais/Wallis, Sion 1950, Switzerland
2
Mabritec AG, Food Technology, Riehen 4125, Switzerland
3
Spiez Laboratory, Federal Office for Civil Protection, Spiez 3700, Switzerland
4
Plants and Pathogens Group, Research Institute Land Nature Environment School of, Architecture and Landscape HEPIA HES-SO University of Applied Sciences and Arts Western Switzerland, 150 route de Presinge, 1254 Geneva/Jussy, Switzerland
*
Author to whom correspondence should be addressed.
Received: 11 December 2018 / Revised: 1 February 2019 / Accepted: 6 February 2019 / Published: 9 February 2019
PDF [579 KB, uploaded 9 February 2019]

Abstract

Routine identification of pathogens by MALDI-TOF MS (matrix-assisted laser desorption ionisation time-of-flight mass spectrometry) is based on the fingerprint of intracellular proteins. This work evaluated the use of MALDI-TOF MS for the identification of extracellular pathogen factors. A Staphylococcus aureus isolate from a food contaminant was exponentially grown in liquid cultures. Secreted proteins were collected using methanol– chloroform precipitation and analysed by MALDI-TOF MS. A main peak m/z 28,250 was demonstrated, which was identified as S.aureus enterotoxin type B (SEB) by using the pure authentic SEB reference of 28.2 kDa and by amino acid sequence analysis. SEB was also detected in this intact form following pasteurization and cooking treatments. Further application of the elaborated MALDI-TOF MS protocol resulted in the detection of SEA at m/z 27,032 and SEC at m/z 27,629. In conclusion, a simple sample preparation from S.aureus cultures and an easy-to-perform identification of pathogen factors SE in intact form represents a promising next-generation application of MALDI-TOF MS.
Keywords: staphylococcal enterotoxins; superantigen; MALDI-TOF MS staphylococcal enterotoxins; superantigen; MALDI-TOF MS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Tonacini, J.; Stephan, D.; Vogel, G.; Avondet, M.-A.; Kalman, F.; Crovadore, J.; Lefort, F.; Schnyder, B. Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry. Toxins 2019, 11, 101.

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