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Open AccessArticle

The Latoia consocia Caterpillar Induces Pain by Targeting Nociceptive Ion Channel TRPV1

by Zhihao Yao 1,†, Peter Muiruri Kamau 2,3,4,†, Yalan Han 2,3,†, Jingmei Hu 5, Anna Luo 2,3, Lei Luo 2,*, Jie Zheng 6,*, Yuhua Tian 1,* and Ren Lai 2,4,5,7,8,9,*
1
Department of Pharmacology, Qingdao University School of Pharmacy, Qingdao 266000, China
2
Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of bioactive peptides of Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China
3
University of Chinese Academy of Sciences, Beijing 100049, China
4
Sino-African Joint Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
5
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
6
Department of Physiology and Membrane Biology, University of California, Davis, CA 95616, USA
7
KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
8
Institute for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China
9
Center for Biosafety Mega-Science, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuchang District/Huangjin Industrial Park, Zhengdian Street, Jiangxia District, Wuhan 430207, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Toxins 2019, 11(12), 695; https://doi.org/10.3390/toxins11120695
Received: 20 October 2019 / Revised: 16 November 2019 / Accepted: 25 November 2019 / Published: 27 November 2019
(This article belongs to the Special Issue Animal Venoms and Their Components: Molecular Mechanisms of Action)
Accidental contact with caterpillar bristles causes local symptoms such as severe pain, intense heat, edema, erythema, and pruritus. However, there is little functional evidence to indicate a potential mechanism. In this study, we analyzed the biological characteristics of the crude venom from the larval stage of Latoia consocia living in South-West China. Intraplantar injection of the venom into the hind paws of mice induced severe acute pain behaviors in wild type (WT) mice; the responses were much reduced in TRPV1-deficit (TRPV1 KO) mice. The TRPV1-specific inhibitor, capsazepine, significantly attenuated the pain behaviors. Furthermore, the crude venom evoked strong calcium signals in the dorsal root ganglion (DRG) neurons of WT mice but not those of TRPV1 KO mice. Among the pain-related ion channels we tested, the crude venom only activated the TRPV1 channel. To better understand the venom components, we analyzed the transcriptome of the L. consocia sebaceous gland region. Our study suggests that TRPV1 serves as a primary nociceptor in caterpillar-induced pain and forms the foundation for elucidating the pain-producing mechanism. View Full-Text
Keywords: caterpillar; Latoia consocia; venom; trpv1; pain caterpillar; Latoia consocia; venom; trpv1; pain
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MDPI and ACS Style

Yao, Z.; Kamau, P.M.; Han, Y.; Hu, J.; Luo, A.; Luo, L.; Zheng, J.; Tian, Y.; Lai, R. The Latoia consocia Caterpillar Induces Pain by Targeting Nociceptive Ion Channel TRPV1. Toxins 2019, 11, 695.

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