Next Article in Journal
Toxin Production in Soybean (Glycine max L.) Plants with Charcoal Rot Disease and by Macrophomina phaseolina, the Fungus that Causes the Disease
Previous Article in Journal
A Review of the Effect of Trace Metals on Freshwater Cyanobacterial Growth and Toxin Production
Open AccessArticle

Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets

1
Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria
2
BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria
*
Author to whom correspondence should be addressed.
Current address: Toxalim, Research Center in Food Toxicology, Université de Toulouse, INRA, UMR 1331, ENVT, INP-Purpan Toulouse, France
Toxins 2019, 11(11), 644; https://doi.org/10.3390/toxins11110644
Received: 29 September 2019 / Revised: 27 October 2019 / Accepted: 1 November 2019 / Published: 5 November 2019
(This article belongs to the Section Mycotoxins)
The Fusarium mycotoxin deoxynivalenol (DON) contaminates animal feed worldwide. In vivo, DON modifies the cellular protein synthesis, thereby also affecting the immune system. However, the functional consequences of this are still ill-defined. In this study, peripheral blood mononuclear cells from healthy pigs were incubated with different DON concentrations in the presence of Concanavalin A (ConA), a plant-derived polyclonal T-cell stimulant. T-cell subsets were investigated for proliferation and expression of CD8α, CD27, and CD28, which are involved in activation and costimulation of porcine T cells. A clear decrease in proliferation of all ConA-stimulated major T-cell subsets (CD4+, CD8+, and γδ T cells) was observed in DON concentrations higher than 0.4 µM. This applied in particular to naïve CD4+ and CD8+ T cells. From 0.8 μM onwards, DON induced a reduction of CD8α (CD4+) and CD27 expression (CD4+ and CD8+ T cells). CD28 expression was diminished in CD4+ and CD8+ T cells at a concentration of 1.6 µM DON. None of these effects were observed with the DON-derivative deepoxy-deoxynivalenol (DOM-1) at 16 µM. These results indicate that DON reduces T-cell proliferation and the expression of molecules involved in T-cell activation, providing a molecular basis for some of the described immunosuppressive effects of DON.
Keywords: deoxynivalenol; in vitro; pig; T cells; proliferation; costimulatory molecules; CD27; CD28 deoxynivalenol; in vitro; pig; T cells; proliferation; costimulatory molecules; CD27; CD28
MDPI and ACS Style

Vatzia, E.; Pierron, A.; Saalmüller, A.; Mayer, E.; Gerner, W. Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets. Toxins 2019, 11, 644.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop