Next Article in Journal
An Antiviral Peptide from Alopecosa nagpag Spider Targets NS2B–NS3 Protease of Flaviviruses
Previous Article in Journal
Effects of Claudin-1 on the Action of Clostridium perfringens Enterotoxin in Caco-2 Cells
Open AccessArticle

An Automated and High-Throughput Immunoaffinity Magnetic Bead-Based Sample Clean-Up Platform for the Determination of Aflatoxins in Grains and Oils Using UPLC-FLD

Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, China
*
Authors to whom correspondence should be addressed.
Toxins 2019, 11(10), 583; https://doi.org/10.3390/toxins11100583
Received: 12 September 2019 / Revised: 4 October 2019 / Accepted: 6 October 2019 / Published: 10 October 2019
Sample clean-up remains the most time-consuming and error-prone step in the whole analytical procedure for aflatoxins (AFTs) analysis. Herein, an automated and high-throughput sample clean-up platform was developed with a disposable, cost-effective immunoaffinity magnetic bead-based kit. Under optimized conditions, the automated method takes less than 30 min to simultaneously purify 20 samples without requiring any centrifugation or filtering steps. When coupled to ultra-high performance liquid chromatography with fluorescence detection, this new analysis method displays excellent accuracy and precision as well as outstanding efficiency. Furthermore, an interlaboratory study was performed in six laboratories to validate the novel protocol. Mean recovery, repeatability, reproducibility, and Horwitz ratio values were within 91.9%–107.4%, 2.5%–7.4%, 2.7%–10.6%, and 0.26%–0.90, respectively. Results demonstrate that the developed sample clean-up platform is a reliable alternative to most widely adopted clean-up procedures for AFTs in cereals and oils. View Full-Text
Keywords: Aflatoxins; immunoaffinity magnetic beads; automated and high-throughput sample clean-up; ultra-high performance liquid chromatography with fluorescence detection (UPLC-FLD). Aflatoxins; immunoaffinity magnetic beads; automated and high-throughput sample clean-up; ultra-high performance liquid chromatography with fluorescence detection (UPLC-FLD).
Show Figures

Graphical abstract

MDPI and ACS Style

Xuan, Z.; Ye, J.; Zhang, B.; Li, L.; Wu, Y.; Wang, S. An Automated and High-Throughput Immunoaffinity Magnetic Bead-Based Sample Clean-Up Platform for the Determination of Aflatoxins in Grains and Oils Using UPLC-FLD. Toxins 2019, 11, 583.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop