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Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit

1,†
,
2,†
,
1
,
2
and
1,*
1
Institute of Environmental Health and Ecological Security, School of the Environment and Safety Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China
2
Institute of Plant Protection and Agro-Product Safety, Anhui Academy of Agricultural Sciences, Key Laboratory of Agro-Product Safety Risk Evaluation (Hefei), Ministry of Agriculture, South Road of agricultural science 40, Hefei 230031, China
Both authors contributed equally to this paper.
*
Author to whom correspondence should be addressed.
Received: 12 November 2018 / Revised: 16 December 2018 / Accepted: 21 December 2018 / Published: 24 December 2018
(This article belongs to the Special Issue Advanced Methods for Mycotoxins Detection)
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Abstract

A total of 328 agricultural product samples highly suspected to be contaminated, from flour companies, feed companies, and livestock farms throughout China, were surveyed for deoxynivalenol (DON) contamination using a self-assembly enzyme-linked immunosorbent assay (ELISA) kit. An ELISA kit for DON was developed with a 4.9 ng mL−1 limit of detection (LOD) in working buffer and a 200 ng g−1 LOD in authentic samples. The DON contamination detection rate was 88.7%, concentrations ranged from 200.9 to 6480.6 ng g−1, and the highest DON contamination was found in distillers’ dried grains with solubles with an average of 3204.5 ng g−1. Wheat bran and wheat were found to be the most commonly contaminated samples, and the corn meal samples had the lowest average DON level. This ELISA kit is a powerful alternative method for the rapid, sensitive, specific, accurate, and high-throughput determination of DON and can meet the maximum requirement levels. This survey suggests that DON contamination in the Chinese market is serious, and the contamination risk deserves attention. Essential preventive measures should be implemented to ensure food safety and human health. View Full-Text
Keywords: deoxynivalenol; contamination; survey; ELISA kit; agricultural product deoxynivalenol; contamination; survey; ELISA kit; agricultural product
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Li, M.; Sun, M.; Hong, X.; Duan, J.; Du, D. Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit. Toxins 2019, 11, 6.

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