Removal of Microcystin-LR by a Novel Native Effective Bacterial Community Designated as YFMCD4 Isolated from Lake Taihu
AbstractMicrocystin-LR (MC-LR) is the most toxic and frequently detected monocyclic heptapeptide hepatotoxin produced by cyanobacteria, which poses a great threat to the natural ecosystem and public health. It is very important to seek environment-friendly and cost-efficient methods to remove MC-LR in water. In this study, the MC-degrading capacities of a novel indigenous bacterial community designated as YFMCD4 and the influence of environmental factors including various temperatures, MC concentrations and pH on the MC-degrading activities were investigated utilizing high-performance liquid chromatography (HPLC). In addition, the MC-degrading mechanism of YFMCD4 was also studied using HPLC coupled with a mass spectrometry equipped with electrospray ionization interface (HPLC-ESI-MS). The data showed MC-LR was completely removed at the maximum rate of 0.5 µg/(mL·h) under the optimal condition by YFMCD4. Two pure bacterial strains Alcaligenes faecalis and Stenotrophomonas acidaminiohila were isolated from YFMCD4 degraded MC-LR at a slower rate. The MC-degrading rates of YFMCD4 were significantly affected by different temperatures, pH and MC-LR concentrations. Two intermediates of a tetrapeptide and Adda appeared in the degradation process. These results illustrate that the novel YFMCD4 is one of the highest effective MC-degrading bacterial community, which can completely remove MC-LR and possesses a significant potential to treat water bodies contaminated by MC-LR. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 189 KB)
Share & Cite This Article
Yang, F.; Guo, J.; Huang, F.; Massey, I.Y.; Huang, R.; Li, Y.; Wen, C.; Ding, P.; Zeng, W.; Liang, G. Removal of Microcystin-LR by a Novel Native Effective Bacterial Community Designated as YFMCD4 Isolated from Lake Taihu. Toxins 2018, 10, 363.
Yang F, Guo J, Huang F, Massey IY, Huang R, Li Y, Wen C, Ding P, Zeng W, Liang G. Removal of Microcystin-LR by a Novel Native Effective Bacterial Community Designated as YFMCD4 Isolated from Lake Taihu. Toxins. 2018; 10(9):363.Chicago/Turabian Style
Yang, Fei; Guo, Jian; Huang, Feiyu; Massey, Isaac Y.; Huang, Ruixue; Li, Yunhui; Wen, Cong; Ding, Ping; Zeng, Weiming; Liang, Geyu. 2018. "Removal of Microcystin-LR by a Novel Native Effective Bacterial Community Designated as YFMCD4 Isolated from Lake Taihu." Toxins 10, no. 9: 363.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.