Detoxification- and Immune-Related Transcriptomic Analysis of Gills from Bay Scallops (Argopecten irradians) in Response to Algal Toxin Okadaic Acid
Abstract
:1. Introduction
2. Results
2.1. Analysis of DGE Libraries
2.2. Functional Annotation and Species Distribution
2.3. Differential Gene Expression Analysis
2.4. Enrichment and Pathway Analysis
2.5. Identification of Genes Related to OA-Induced Stress Response
3. Discussion
4. Materials and Methods
4.1. Maintenance of Scallops
4.2. Okadaic Acid Exposure and RNA Extraction
4.3. Library Preparation and Illumina Sequencing
4.4. De Novo Transcriptome Assembly
4.5. Gene Annotation and Analysis
4.6. Differential Expression Analysis
4.7. GO and KEGG Enrichment Analysis of Differentially Expressed Genes
4.8. Quantitative Real-Time PCR Validation
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample | Total Number | Total Length | Mean Length | N50 | N70 | N90 | GC(%) |
---|---|---|---|---|---|---|---|
Control | 51,465 | 41,105,722 | 798 | 1411 | 704 | 302 | 39.48 |
OA-treated | 49,453 | 43,129,157 | 872 | 1646 | 803 | 318 | 39.63 |
All-unigene | 55,876 | 53,465,429 | 956 | 1840 | 960 | 345 | 39.42 |
Function | Transcript | Log2 (Fold Change) (RNAseq) | Regulation |
---|---|---|---|
Immune system | C-type lectin superfamily 17 member A | −4.255 | Down |
C-type lectin domain family 4 member E | −3.507 | Down | |
Complement C1q tumor necrosis factor-related protein 2 | −4.791 | Down | |
Fibrinogen C domain-containing protein 1 | −2.100 | Down | |
Toll-like receptor 4 | 2.880 | Up | |
Toll-like receptor 13 | 1.347 | Up | |
Acid phosphatase | 2.238 | Up | |
NADPH oxidase 3 | 2.493 | Up | |
Detoxification | ATP-binding cassette, subfamily C, member 1 | 1.773 | Up |
ATP-binding cassette sub-family B member 10 | 1.165 | Up | |
ATP-binding cassette, sub-family C member 5 | 1.280 | Up | |
Cyclic AMP-responsive element-binding protein | 1.953 | Up | |
Nuclear factor erythroid 2-related factor 2 | 1.231 | Up | |
NADPH2:quinone reductase | 1.677 | Up | |
Cytochrome P450 3A80 | 1.207 | Up | |
Cytochrome P450 3A64 | 1.783 | Up | |
Cytochrome P450 1A5 | −1.686 | Down | |
Cytochrome P450 3A24 | −2.315 | Down | |
Superoxide dismutase Cu-Zn family | 1.139 | Up | |
Superoxide dismutase 2 | −1.126 | Down | |
Glutathione S-transferase 1 | −1.552 | Down | |
Glutathione S-transferase 2 | −2.511 | Down | |
Glutathione S-transferase omega | −1.775 | Down | |
Glutathione S-transferase theta-1 | −1.254 | Down | |
Glutathione S-transferase A | −1.218 | Down | |
Glutathione S-transferase kappa | −2.356 | Down |
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Chi, C.; Giri, S.S.; Jun, J.W.; Kim, S.W.; Kim, H.J.; Kang, J.W.; Park, S.C. Detoxification- and Immune-Related Transcriptomic Analysis of Gills from Bay Scallops (Argopecten irradians) in Response to Algal Toxin Okadaic Acid. Toxins 2018, 10, 308. https://doi.org/10.3390/toxins10080308
Chi C, Giri SS, Jun JW, Kim SW, Kim HJ, Kang JW, Park SC. Detoxification- and Immune-Related Transcriptomic Analysis of Gills from Bay Scallops (Argopecten irradians) in Response to Algal Toxin Okadaic Acid. Toxins. 2018; 10(8):308. https://doi.org/10.3390/toxins10080308
Chicago/Turabian StyleChi, Cheng, Sib Sankar Giri, Jin Woo Jun, Sang Wha Kim, Hyoun Joong Kim, Jeong Woo Kang, and Se Chang Park. 2018. "Detoxification- and Immune-Related Transcriptomic Analysis of Gills from Bay Scallops (Argopecten irradians) in Response to Algal Toxin Okadaic Acid" Toxins 10, no. 8: 308. https://doi.org/10.3390/toxins10080308