The Effect of Botulinum Neurotoxin Serotype a Heavy Chain on the Growth Related Proteins and Neurite Outgrowth after Spinal Cord Injury in Rats
Abstract
1. Introduction
2. Results
2.1. Unilateral Lumbar SCI Model and Spinal Function Evaluation
2.2. The Alteration of Local Protein Expression after SCI and the Effect of BoNT/A HC on It
2.3. Effects of BoNT/A Heavy Chain on the Expression of Selective Growth-Associated Proteins
2.4. The Distribution of Positive GAP43 and SCG10 Immunofluorescence at the Injured Area after Administration of the BoNT/A HC
2.5. The Measurement of SCG 10 Positive Neuronal Processes
2.6. The Amelioration of Motor and Sensory Function by BoNT/A HC Treatment Based on SCI
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Establishing the Rat Spinal Cord Hemi-Section Injury Model
5.2. BoNT/A Heavy Chain Administration and Groups
5.3. Two-Dimensional Gel Electrophoresis
5.4. SDS-PAGE and Western Blot
5.5. Immunofluorescence Staining
5.6. Measurement of Neuronal Processes
5.7. Behavioral Evaluation
5.8. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Groups | 0 d | 2 d | 1 w | 2 w | 4 w |
---|---|---|---|---|---|
Control | 8.5 ± 0.2 | 8.5 ± 0.2 | 8.3 ± 0.1 | 7.8 ± 0.2 | 8.2 ± 0.2 |
Injury | 8.5 ± 0.2 | 22.8 ± 1.0 # | 22.1 ± 0.9 # | 22.4 ± 1.0 # | 21.5 ± 0.9 # |
BoNT/A HC | 8.5 ± 0.2 | 24.4 ± 0.3 | 24.5 ± 0.3 | 24.1 ± 0.4 | 22.5 ± 0.9 |
Name of Group | Pseudo Surgery | SCI without BoNT/A HC | SCI with BoNT/A HC | ||||||
---|---|---|---|---|---|---|---|---|---|
Time | 0 | 2 d | 1 wk | 2 wks | 4 wks | 2 d | 1 wk | 2 wks | 4 wks |
No. of animals | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
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Wang, Y.-F.; Liu, F.; Lan, J.; Bai, J.; Li, X.-Q. The Effect of Botulinum Neurotoxin Serotype a Heavy Chain on the Growth Related Proteins and Neurite Outgrowth after Spinal Cord Injury in Rats. Toxins 2018, 10, 66. https://doi.org/10.3390/toxins10020066
Wang Y-F, Liu F, Lan J, Bai J, Li X-Q. The Effect of Botulinum Neurotoxin Serotype a Heavy Chain on the Growth Related Proteins and Neurite Outgrowth after Spinal Cord Injury in Rats. Toxins. 2018; 10(2):66. https://doi.org/10.3390/toxins10020066
Chicago/Turabian StyleWang, Ya-Fang, Fu Liu, Jing Lan, Juan Bai, and Xia-Qing Li. 2018. "The Effect of Botulinum Neurotoxin Serotype a Heavy Chain on the Growth Related Proteins and Neurite Outgrowth after Spinal Cord Injury in Rats" Toxins 10, no. 2: 66. https://doi.org/10.3390/toxins10020066
APA StyleWang, Y.-F., Liu, F., Lan, J., Bai, J., & Li, X.-Q. (2018). The Effect of Botulinum Neurotoxin Serotype a Heavy Chain on the Growth Related Proteins and Neurite Outgrowth after Spinal Cord Injury in Rats. Toxins, 10(2), 66. https://doi.org/10.3390/toxins10020066