The Effect of Botulinum Neurotoxin Serotype a Heavy Chain on the Growth Related Proteins and Neurite Outgrowth after Spinal Cord Injury in Rats
Abstract
:1. Introduction
2. Results
2.1. Unilateral Lumbar SCI Model and Spinal Function Evaluation
2.2. The Alteration of Local Protein Expression after SCI and the Effect of BoNT/A HC on It
2.3. Effects of BoNT/A Heavy Chain on the Expression of Selective Growth-Associated Proteins
2.4. The Distribution of Positive GAP43 and SCG10 Immunofluorescence at the Injured Area after Administration of the BoNT/A HC
2.5. The Measurement of SCG 10 Positive Neuronal Processes
2.6. The Amelioration of Motor and Sensory Function by BoNT/A HC Treatment Based on SCI
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Establishing the Rat Spinal Cord Hemi-Section Injury Model
5.2. BoNT/A Heavy Chain Administration and Groups
5.3. Two-Dimensional Gel Electrophoresis
5.4. SDS-PAGE and Western Blot
5.5. Immunofluorescence Staining
5.6. Measurement of Neuronal Processes
5.7. Behavioral Evaluation
5.8. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Groups | 0 d | 2 d | 1 w | 2 w | 4 w |
---|---|---|---|---|---|
Control | 8.5 ± 0.2 | 8.5 ± 0.2 | 8.3 ± 0.1 | 7.8 ± 0.2 | 8.2 ± 0.2 |
Injury | 8.5 ± 0.2 | 22.8 ± 1.0 # | 22.1 ± 0.9 # | 22.4 ± 1.0 # | 21.5 ± 0.9 # |
BoNT/A HC | 8.5 ± 0.2 | 24.4 ± 0.3 | 24.5 ± 0.3 | 24.1 ± 0.4 | 22.5 ± 0.9 |
Name of Group | Pseudo Surgery | SCI without BoNT/A HC | SCI with BoNT/A HC | ||||||
---|---|---|---|---|---|---|---|---|---|
Time | 0 | 2 d | 1 wk | 2 wks | 4 wks | 2 d | 1 wk | 2 wks | 4 wks |
No. of animals | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
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Wang, Y.-F.; Liu, F.; Lan, J.; Bai, J.; Li, X.-Q. The Effect of Botulinum Neurotoxin Serotype a Heavy Chain on the Growth Related Proteins and Neurite Outgrowth after Spinal Cord Injury in Rats. Toxins 2018, 10, 66. https://doi.org/10.3390/toxins10020066
Wang Y-F, Liu F, Lan J, Bai J, Li X-Q. The Effect of Botulinum Neurotoxin Serotype a Heavy Chain on the Growth Related Proteins and Neurite Outgrowth after Spinal Cord Injury in Rats. Toxins. 2018; 10(2):66. https://doi.org/10.3390/toxins10020066
Chicago/Turabian StyleWang, Ya-Fang, Fu Liu, Jing Lan, Juan Bai, and Xia-Qing Li. 2018. "The Effect of Botulinum Neurotoxin Serotype a Heavy Chain on the Growth Related Proteins and Neurite Outgrowth after Spinal Cord Injury in Rats" Toxins 10, no. 2: 66. https://doi.org/10.3390/toxins10020066