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Article

Complexes of the Mycotoxins Citrinin and Ochratoxin A with Aluminum Ions and their Spectroscopic Properties

Division of Organic Trace and Food Analysis, Department of Analytical Chemistry, Reference Materials, Bundesanstalt für Materialforschung und-prüfung (BAM), Richard-Willstätter-Str. 11, D-12489 Berlin, Germany
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Toxins 2018, 10(12), 538; https://doi.org/10.3390/toxins10120538
Received: 19 November 2018 / Revised: 9 December 2018 / Accepted: 13 December 2018 / Published: 14 December 2018
(This article belongs to the Section Mycotoxins)
The sensitive detection of the mycotoxin citrinin (CIT) utilizing its fluorescence requires approaches to enhance the emission. In this respect, we studied the complexation of CIT and ochratoxin A (OTA) with Al3+ in methanol using absorption and fluorescence spectroscopy. In this context, an isocratic high performance liquid chromatography (HPLC) method using a polymer column and a fluorescence detector was also developed that enables the separation of the metal ion complexes from the free ligands and non-complexed Al3+. CIT and OTA showed distinct changes in their absorption and fluorescence properties upon Al3+-coordination, and the fluorescence of CIT was considerably enhanced. Analysis of the photometrically assessed titration of CIT and OTA with Al3+ using the Job plot method revealed 1:2 and 1:1 stoichiometries for the Al3+ complexes of CIT (Al:CIT) and OTA (Al:OTA), respectively. In the case of CIT, only one β-diketone moiety participates in Al3+ coordination. These findings can be elegantly exploited for signal amplification and provide the base to reduce the limit of detection for CIT quantification by about an order of magnitude, as revealed by HPLC measurements using a fluorescence detector. View Full-Text
Keywords: complexation; aluminum; fluorescence; Job plot; HPLC-DAD/FLD complexation; aluminum; fluorescence; Job plot; HPLC-DAD/FLD
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MDPI and ACS Style

Keller, J.; Moldenhauer, D.; Byrne, L.; Haase, H.; Resch-Genger, U.; Koch, M. Complexes of the Mycotoxins Citrinin and Ochratoxin A with Aluminum Ions and their Spectroscopic Properties. Toxins 2018, 10, 538. https://doi.org/10.3390/toxins10120538

AMA Style

Keller J, Moldenhauer D, Byrne L, Haase H, Resch-Genger U, Koch M. Complexes of the Mycotoxins Citrinin and Ochratoxin A with Aluminum Ions and their Spectroscopic Properties. Toxins. 2018; 10(12):538. https://doi.org/10.3390/toxins10120538

Chicago/Turabian Style

Keller, Julia, Daniel Moldenhauer, Liam Byrne, Hajo Haase, Ute Resch-Genger, and Matthias Koch. 2018. "Complexes of the Mycotoxins Citrinin and Ochratoxin A with Aluminum Ions and their Spectroscopic Properties" Toxins 10, no. 12: 538. https://doi.org/10.3390/toxins10120538

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