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Toxins 2018, 10(11), 441; https://doi.org/10.3390/toxins10110441

Investigation into Cellular Glycolysis for the Mechanism Study of Energy Metabolism Disorder Triggered by Lipopolysaccharide

1
School of Food Science, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Foods, Jiangnan University, Wuxi 214122, China
2
Synergetic Innovation Center for Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China
3
NIHWest Coast Metabolomics Center, UC Davis Genome Center, University of California, Davis, CA 95616, USA
*
Author to whom correspondence should be addressed.
Received: 15 September 2018 / Revised: 9 October 2018 / Accepted: 16 October 2018 / Published: 29 October 2018
(This article belongs to the Special Issue Lipopolysaccharide: Bacterial Endotoxin)
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Abstract

Lipopolysaccharide (LPS) is the main virulence factor of Gram-negative bacteria, which can incite inflammation in tissues by inducing cells to secrete a variety of proinflammatory mediators, including cytokines, chemokines, interleukins, and prostaglandins. Herein, we chose LPS as an inducer to establish an inflammatory model of HeLa cells, and explored the effects of LPS on energy metabolism. We treated HeLa cells with different concentrations (0, 0.4, 1.0, 2.0, 4.0, and 6.0 μg/mL) of LPS for 24 h, and explored its effects on intercellular adenosine triphosphate (ATP) levels, intercellular nitrous oxide (NO) content, mitochondrial functions, and enzyme activities related to energy metabolism. Furthermore, we used metabonomics to study the metabolites that participated in energy metabolism. We found a positive correlation between LPS concentrations and intracellular ATP levels. In addition, LPS increased intracellular NO production, altered mitochondrial functions, strengthened glycolytic enzyme activities, and changed metabolites related to energy metabolism. Hence, in this study, we showed that LPS can strengthen energy metabolism by enhancing glycolysis, which could be used as an early diagnostic biomarker or a novel therapeutic target for inflammation-associated cancers. View Full-Text
Keywords: lipopolysaccharide; energy metabolism; glycolysis; cancer lipopolysaccharide; energy metabolism; glycolysis; cancer
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Zhang, R.; Ji, J.; Blaženović, I.; Pi, F.; Wang, T.; Zhang, Y.; Sun, X. Investigation into Cellular Glycolysis for the Mechanism Study of Energy Metabolism Disorder Triggered by Lipopolysaccharide. Toxins 2018, 10, 441.

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