Next Article in Journal
Streptomyces roseolus, A Promising Biocontrol Agent Against Aspergillus flavus, the Main Aflatoxin B1 Producer
Previous Article in Journal
iTRAQ-Based Quantitative Proteomic Analysis Reveals Changes in Metabolite Biosynthesis in Monascus purpureus in Response to a Low-Frequency Magnetic Field
Previous Article in Special Issue
Lipopolysaccharide Structural Differences between Western and Asian Helicobacter pylori Strains
Article Menu

Export Article

Open AccessArticle
Toxins 2018, 10(11), 441;

Investigation into Cellular Glycolysis for the Mechanism Study of Energy Metabolism Disorder Triggered by Lipopolysaccharide

School of Food Science, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Foods, Jiangnan University, Wuxi 214122, China
Synergetic Innovation Center for Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China
NIHWest Coast Metabolomics Center, UC Davis Genome Center, University of California, Davis, CA 95616, USA
Author to whom correspondence should be addressed.
Received: 15 September 2018 / Revised: 9 October 2018 / Accepted: 16 October 2018 / Published: 29 October 2018
(This article belongs to the Special Issue Lipopolysaccharide: Bacterial Endotoxin)
Full-Text   |   PDF [3448 KB, uploaded 29 October 2018]   |  


Lipopolysaccharide (LPS) is the main virulence factor of Gram-negative bacteria, which can incite inflammation in tissues by inducing cells to secrete a variety of proinflammatory mediators, including cytokines, chemokines, interleukins, and prostaglandins. Herein, we chose LPS as an inducer to establish an inflammatory model of HeLa cells, and explored the effects of LPS on energy metabolism. We treated HeLa cells with different concentrations (0, 0.4, 1.0, 2.0, 4.0, and 6.0 μg/mL) of LPS for 24 h, and explored its effects on intercellular adenosine triphosphate (ATP) levels, intercellular nitrous oxide (NO) content, mitochondrial functions, and enzyme activities related to energy metabolism. Furthermore, we used metabonomics to study the metabolites that participated in energy metabolism. We found a positive correlation between LPS concentrations and intracellular ATP levels. In addition, LPS increased intracellular NO production, altered mitochondrial functions, strengthened glycolytic enzyme activities, and changed metabolites related to energy metabolism. Hence, in this study, we showed that LPS can strengthen energy metabolism by enhancing glycolysis, which could be used as an early diagnostic biomarker or a novel therapeutic target for inflammation-associated cancers. View Full-Text
Keywords: lipopolysaccharide; energy metabolism; glycolysis; cancer lipopolysaccharide; energy metabolism; glycolysis; cancer

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Zhang, R.; Ji, J.; Blaženović, I.; Pi, F.; Wang, T.; Zhang, Y.; Sun, X. Investigation into Cellular Glycolysis for the Mechanism Study of Energy Metabolism Disorder Triggered by Lipopolysaccharide. Toxins 2018, 10, 441.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top