Purification and Characterization of JZTx-14, a Potent Antagonist of Mammalian and Prokaryotic Voltage-Gated Sodium Channels
Abstract
:1. Introduction
2. Results
2.1. Purification and Characterization of JZTx-14
2.2. JZTx-14 Is a Potent but Non-Selective Mammalian NaVs Toxin
2.3. JZTx-14 Acts on Mammalian NaVs as a Gating Modifier
2.4. Effects of JZTx-14 on Bacterial NaVs
2.5. The Molecular Mechanism of JZTx-14 Interacting with NaChBac
3. Discussion
4. Materials and Methods
4.1. Toxin Purification and N-Terminal Sequence Determination
4.2. Mass Spectrometric Analysis and Toxin C-Terminal Sequence Determination
4.3. Constructs and Transfection
4.4. Whole-Cell Patch Clamp Recordings
4.5. Data Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Zhang, J.; Tang, D.; Liu, S.; Hu, H.; Liang, S.; Tang, C.; Liu, Z. Purification and Characterization of JZTx-14, a Potent Antagonist of Mammalian and Prokaryotic Voltage-Gated Sodium Channels. Toxins 2018, 10, 408. https://doi.org/10.3390/toxins10100408
Zhang J, Tang D, Liu S, Hu H, Liang S, Tang C, Liu Z. Purification and Characterization of JZTx-14, a Potent Antagonist of Mammalian and Prokaryotic Voltage-Gated Sodium Channels. Toxins. 2018; 10(10):408. https://doi.org/10.3390/toxins10100408
Chicago/Turabian StyleZhang, Jie, Dongfang Tang, Shuangyu Liu, Haoliang Hu, Songping Liang, Cheng Tang, and Zhonghua Liu. 2018. "Purification and Characterization of JZTx-14, a Potent Antagonist of Mammalian and Prokaryotic Voltage-Gated Sodium Channels" Toxins 10, no. 10: 408. https://doi.org/10.3390/toxins10100408