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Transketolase Is Identified as a Target of Herbicidal Substance α-Terthienyl by Proteomics

College of plant protection, Agricultural University of Hebei, Baoding 071000, China
College of life science, Agricultural University of Hebei, Baoding 071000, China
Authors to whom correspondence should be addressed.
Toxins 2018, 10(1), 41;
Received: 28 November 2017 / Revised: 27 December 2017 / Accepted: 11 January 2018 / Published: 12 January 2018
(This article belongs to the Section Plant Toxins)
PDF [4007 KB, uploaded 12 January 2018]


α-terthienyl is a natural phytotoxin isolated originally from Flaveria bidentis (L.) Kuntze. The bioassay presented here shows the strong herbicidal activity of α-terthienyl on Digitaria sanguinalis, Arabidopsis thaliana and Chlamydomonas reinhardtii. The α-terthienyl-induced response of A. thaliana at the protein level was analyzed at different times. Changes in the protein expression profiles were analyzed by two-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry (LC-MS/MS) mass spectrometry. Sixteen protein spots were identified that showed reproducible changes in the expression of at least 2-fold when compared to the control. Among these 16 spots, three were up-regulated and 13 were down-regulated. The decreased expression of several proteins associated with energy production and carbon metabolism suggested that these processes were affected by α-terthienyl. To search for the candidate proteins in this screen, A. thaliana T-DNA mutants of the candidate proteins were used to test their susceptibility to α-terthienyl. Amongst the others, attkl1, a mutant of transketolase, exhibited a significantly lower sensitivity to α-terthienyl when hit compared with Col-0. Based on the identification of the proteins associated with the response to α-terthienyl by proteomics, a candidate target protein transketolase was identified. View Full-Text
Keywords: α-terthienyl; proteomics; herbicidal mechanism; transketolase α-terthienyl; proteomics; herbicidal mechanism; transketolase

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Zhao, B.; Huo, J.; Liu, N.; Zhang, J.; Dong, J. Transketolase Is Identified as a Target of Herbicidal Substance α-Terthienyl by Proteomics. Toxins 2018, 10, 41.

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