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Calcium Supplement Derived from Gallus gallus domesticus Promotes BMP-2/RUNX2/SMAD5 and Suppresses TRAP/RANK Expression through MAPK Signaling Activation

1
Department of Food Science and Technology, Seoul National University of Science & Technology, Seoul 01811, Korea
2
Department of Chemical & Biomolecular Engineering, Sogang University, Seoul 04170, Korea
3
Department of Advanced Materials Engineering, Daejeon University, Daejeon 34520, Korea
4
Department of Oriental Medicine, Daejeon University, Daejeon 34520, Korea
5
Division of Food Bioscience, Konkuk University, Chungju 27478, Korea
*
Author to whom correspondence should be addressed.
Nutrients 2017, 9(5), 504; https://doi.org/10.3390/nu9050504
Received: 21 March 2017 / Revised: 28 April 2017 / Accepted: 12 May 2017 / Published: 17 May 2017
The present study evaluated the effects of a calcium (Ca) supplement derived from Gallus gallus domesticus (GD) on breaking force, microarchitecture, osteogenic differentiation and osteoclast differentiation factor expression in vivo in Ca-deficient ovariectomized (OVX) rats. One percent of Ca supplement significantly improved Ca content and bone strength of the tibia. In micro-computed tomography analysis, 1% Ca supplement attenuated OVX- and low Ca-associated changes in bone mineral density, trabecular thickness, spacing and number. Moreover, 1% Ca-supplemented diet increased the expression of osteoblast differentiation marker genes, such as bone morphogenetic protein-2, Wnt3a, small mothers against decapentaplegic 1/5/8, runt-related transcription factor 2, osteocalcin and collagenase-1, while it decreased the expression of osteoclast differentiation genes, such as thrombospondin-related anonymous protein, cathepsin K and receptor activator of nuclear factor kappa B. Furthermore, 1% Ca-supplemented diet increased the levels of phosphorylated extracellular signal-regulated kinase and c-Jun N-terminal kinase. The increased expression of osteoblast differentiation marker genes and activation of mitogen-activated protein kinase signaling were associated with significant increases in trabecular bone volume, which plays an important role in the overall skeletal strength. Our results demonstrated that 1% Ca supplement inhibited osteoclastogenesis, stimulated osteoblastogenesis and restored bone loss in OVX rats. View Full-Text
Keywords: calcium; osteoporosis; differentiation; ovariectomy rat calcium; osteoporosis; differentiation; ovariectomy rat
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MDPI and ACS Style

Yoo, H.S.; Kim, G.-J.; Song, D.H.; Chung, K.-H.; Lee, K.-J.; Kim, D.-H.; An, J.H. Calcium Supplement Derived from Gallus gallus domesticus Promotes BMP-2/RUNX2/SMAD5 and Suppresses TRAP/RANK Expression through MAPK Signaling Activation. Nutrients 2017, 9, 504. https://doi.org/10.3390/nu9050504

AMA Style

Yoo HS, Kim G-J, Song DH, Chung K-H, Lee K-J, Kim D-H, An JH. Calcium Supplement Derived from Gallus gallus domesticus Promotes BMP-2/RUNX2/SMAD5 and Suppresses TRAP/RANK Expression through MAPK Signaling Activation. Nutrients. 2017; 9(5):504. https://doi.org/10.3390/nu9050504

Chicago/Turabian Style

Yoo, Han S., Gyung-Ji Kim, Da H. Song, Kang-Hyun Chung, Kwon-Jai Lee, Dong-Hee Kim, and Jeung H. An. 2017. "Calcium Supplement Derived from Gallus gallus domesticus Promotes BMP-2/RUNX2/SMAD5 and Suppresses TRAP/RANK Expression through MAPK Signaling Activation" Nutrients 9, no. 5: 504. https://doi.org/10.3390/nu9050504

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