Oleuropein Decreases Cyclooxygenase-2 and Interleukin-17 Expression and Attenuates Inflammatory Damage in Colonic Samples from Ulcerative Colitis Patients
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Sampling
2.2. Ex Vivo Organ Culture
2.3. Extraction of Total Proteins
2.4. Western Blot Analysis
2.5. Measurement of IL-17 in the Culture Supernatants
2.6. Histology
2.7. Statistical Analysis
2.8. Ethical Considerations
3. Results
3.1. OLE Decreases COX-2 Expression in Colonic Biopsy Culture
3.2. OLE Suppresses IL-17 in Both Mucosa and Supernatant of Colonic Biopsy Culture
3.3. OLE Attenuates Inflammatory Damage in Colonic Biopsy Samples
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Characteristic | Values |
---|---|
Sex | 8 males; 6 females |
Age (years) | 59 (range 39–80) |
Mayo Score | 6 (range 4–6) |
Pancolitis | 8 |
Left-sided colitis | 6 |
Oral/Topic Mesalamine | 14 |
Previous azathioprine/anti-TNF alpha | - |
Current oral/topic Steroids | - |
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Share and Cite
Larussa, T.; Oliverio, M.; Suraci, E.; Greco, M.; Placida, R.; Gervasi, S.; Marasco, R.; Imeneo, M.; Paolino, D.; Tucci, L.; Gulletta, E.; Fresta, M.; Procopio, A.; Luzza, F. Oleuropein Decreases Cyclooxygenase-2 and Interleukin-17 Expression and Attenuates Inflammatory Damage in Colonic Samples from Ulcerative Colitis Patients. Nutrients 2017, 9, 391. https://doi.org/10.3390/nu9040391
Larussa T, Oliverio M, Suraci E, Greco M, Placida R, Gervasi S, Marasco R, Imeneo M, Paolino D, Tucci L, Gulletta E, Fresta M, Procopio A, Luzza F. Oleuropein Decreases Cyclooxygenase-2 and Interleukin-17 Expression and Attenuates Inflammatory Damage in Colonic Samples from Ulcerative Colitis Patients. Nutrients. 2017; 9(4):391. https://doi.org/10.3390/nu9040391
Chicago/Turabian StyleLarussa, Tiziana, Manuela Oliverio, Evelina Suraci, Marta Greco, Roberta Placida, Serena Gervasi, Raffaella Marasco, Maria Imeneo, Donatella Paolino, Luigi Tucci, Elio Gulletta, Massimo Fresta, Antonio Procopio, and Francesco Luzza. 2017. "Oleuropein Decreases Cyclooxygenase-2 and Interleukin-17 Expression and Attenuates Inflammatory Damage in Colonic Samples from Ulcerative Colitis Patients" Nutrients 9, no. 4: 391. https://doi.org/10.3390/nu9040391