Next Article in Journal
Lifestyle and Dietary Determinants of Serum Apolipoprotein A1 and Apolipoprotein B Concentrations: Cross-Sectional Analyses within a Swedish Cohort of 24,984 Individuals
Next Article in Special Issue
Antioxidants for Healthy Skin: The Emerging Role of Aryl Hydrocarbon Receptors and Nuclear Factor-Erythroid 2-Related Factor-2
Previous Article in Journal
Effect of 25% Sodium Reduction on Sales of a Top‐Selling Bread in Remote Indigenous Australian  Community Stores: A Controlled Intervention Trial
Previous Article in Special Issue
The Combination of Blueberry Juice and Probiotics Ameliorate Non-Alcoholic Steatohepatitis (NASH) by Affecting SREBP-1c/PNPLA-3 Pathway via PPAR-α
Open AccessArticle

Enantioselective Modulatory Effects of Naringenin  Enantiomers on the Expression Levels of miR‐17‐3p  Involved in Endogenous Antioxidant Defenses

Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, Pavia University, Viale Taramelli 12, 27100 Pavia, Italy
KOLINPHARMA S.p.A., Lainate, Corso Europa 5, 20020 Lainate, Italy
Department of Earth and Environmental Sciences, University of Pavia, Via S. Epifanio 14, 27100 Pavia, Italy
Author to whom correspondence should be addressed.
Nutrients 2017, 9(3), 215;
Received: 28 November 2016 / Accepted: 24 February 2017 / Published: 28 February 2017
(This article belongs to the Special Issue Antioxidants in Health and Disease)
Naringenin is a flavanone present in citrus fruit as a mixture of chiral isomers. The numerous biological properties attributed to this compound include antioxidant and anti‐inflammatory activities, even though the molecular mechanisms of these remain unknown. This study aims to evaluate the effects of racemic and enantiomeric naringenin on the expression levels of miR‐17‐3p, miR‐25‐5p and relative mRNA targets, to elucidate the mechanisms underlying these antioxidant and anti‐inflammatory properties. Caco‐2 cells, a well characterized in vitro model which mimics the intestinal barrier, were treated with subtoxic concentrations of racemate and enantiomers. The expression levels of miR‐17‐3p and miR‐25‐5p were determined by Real‐Time PCR and were found to be decreased for both miRNAs. miR‐17‐3p behavior was in agreement with the increased levels of target mRNAs coding for two antioxidant enzymes, manganese‐dependent superoxide dismutase (MnSOD) and glutathione peroxidase 2 (GPx2), while expression levels of miR‐25‐5p were not in agreement with its target mRNAs, coding for two pro‐inflammatory cytokines, Tumor necrosis factor‐alpha (TNF‐α) and Interleukin‐6 (IL‐6). These results lead to the conclusion that naringenin could exert its antioxidant activity through epigenetic regulation operated by miRNAs, while anti‐inflammatory activity is regulated by other miRNAs and/or mechanisms. View Full-Text
Keywords: naringenin racemate; naringenin enantiomers; antioxidant enzymes; pro‐inflammatory  cytokines; caco‐2 cells; microRNA; epigenetics naringenin racemate; naringenin enantiomers; antioxidant enzymes; pro‐inflammatory  cytokines; caco‐2 cells; microRNA; epigenetics
Show Figures

Figure 1

MDPI and ACS Style

Curti, V.; Di Lorenzo, A.; Rossi, D.; Martino, E.; Capelli, E.; Collina, S.; Daglia, M. Enantioselective Modulatory Effects of Naringenin  Enantiomers on the Expression Levels of miR‐17‐3p  Involved in Endogenous Antioxidant Defenses. Nutrients 2017, 9, 215.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop