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Correction published on 8 September 2020, see Nutrients 2020, 12(9), 2732.
Article

Soy-Based Multiple Amino Acid Oral Supplementation Increases the Anti-Sarcoma Effect of Cyclophosphamide

by 1,2,†, 3, 4,*,† and 1,*,†
1
Department of Life Science, No. 88, Sec. 4, Tingzhou Road, National Taiwan Normal University, Taipei 11677, Taiwan
2
Department of Family Medicine, National Taiwan University Hospital, Taipei 100, Taiwan
3
Biotechnology Center, Grape King Inc., Chung-Li 320, Taiwan
4
Department of Orthopedic, Kuang-Tien General Hospital, Taichung 433, Taiwan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2016, 8(4), 192; https://doi.org/10.3390/nu8040192
Received: 10 February 2016 / Revised: 8 March 2016 / Accepted: 22 March 2016 / Published: 1 April 2016
The use of a mixture of amino acids caused a selective apoptosis induction against a variety of tumor cell lines, reduced the adverse effects of anti-cancer drugs and increased the sensitivity of tumor cells to chemotherapeutic agents. We evaluated the effects and underlying mechanisms of soy-derived multiple amino acids’ oral supplementation on the therapeutic efficacy of low-dose cyclophosphamide (CTX) and on tumor growth, apoptosis, and autophagy in severe combined immunodeficiency (SCID) mice that were injected with sarcoma-180 (S-180) cells. 3-methyladenine or siRNA knockdown of Atg5 was used to evaluate its effect on sarcoma growth. A comparison of mice with implanted sarcoma cells, CTX, and oral saline and mice with implanted sarcoma cells, CTX, and an oral soy-derived multiple amino acid supplement indicated that the soy-derived multiple amino acid supplement significantly decreased overall sarcoma growth, increased the Bax/Bcl-2 ratio, caspase 3 expression, and apoptosis, and depressed LC3 II-mediated autophagy. Treatment with 3-methyladenine or Atg5 siRNA elicited similar responses as CTX plus soy-derived multiple amino acid in downregulating autophagy and upregulating apoptosis. A low dose of CTX combined with an oral soy-derived multiple amino acid supplement had a potent anti-tumor effect mediated through downregulation of autophagy and upregulation of apoptosis. View Full-Text
Keywords: apoptosis; autophagy; Atg5; soy-based amino acids; chemotherapy; mice apoptosis; autophagy; Atg5; soy-based amino acids; chemotherapy; mice
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MDPI and ACS Style

Yao, C.-A.; Chen, C.-C.; Wang, N.-P.; Chien, C.-T. Soy-Based Multiple Amino Acid Oral Supplementation Increases the Anti-Sarcoma Effect of Cyclophosphamide. Nutrients 2016, 8, 192. https://doi.org/10.3390/nu8040192

AMA Style

Yao C-A, Chen C-C, Wang N-P, Chien C-T. Soy-Based Multiple Amino Acid Oral Supplementation Increases the Anti-Sarcoma Effect of Cyclophosphamide. Nutrients. 2016; 8(4):192. https://doi.org/10.3390/nu8040192

Chicago/Turabian Style

Yao, Chien-An, Chin-Chu Chen, Nai-Phog Wang, and Chiang-Ting Chien. 2016. "Soy-Based Multiple Amino Acid Oral Supplementation Increases the Anti-Sarcoma Effect of Cyclophosphamide" Nutrients 8, no. 4: 192. https://doi.org/10.3390/nu8040192

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