Next Article in Journal
Enteral Nutrition Support to Treat Malnutrition in Inflammatory Bowel Disease
Next Article in Special Issue
The Infant Gut Microbiome: Evidence for Obesity Risk and Dietary Intervention
Previous Article in Journal
L-Glutamine and Whole Protein Restore First-Phase Insulin Response and Increase Glucagon-Like Peptide-1 in Type 2 Diabetes Patients
Previous Article in Special Issue
The Controversial Role of Food Allergy in Infantile Colic: Evidence and Clinical Management
Article Menu

Export Article

Open AccessCommunication
Nutrients 2015, 7(4), 2109-2124;

Detection of Sialic Acid-Utilising Bacteria in a Caecal Community Batch Culture Using RNA-Based Stable Isotope Probing

AgResearch Ltd, Food Nutrition & Health Team, Food & Bio-based Products Group, Grasslands Research Centre, Private Bag 11008, Palmerston North 4442, New Zealand
Microbiology & Hygiene Group, Faculty of Medical and Life Sciences, Furtwangen University, 78054 Villingen-Schwenningen, Germany
Arla Strategic Innovation Centre, Roerdrumvej 2, 8220 Brabrand, Denmark
Author to whom correspondence should be addressed.
Received: 30 January 2015 / Accepted: 18 March 2015 / Published: 25 March 2015
(This article belongs to the Special Issue Microbiome and Human Health)
Full-Text   |   PDF [804 KB, uploaded 25 March 2015]   |  


Sialic acids are monosaccharides typically found on cell surfaces and attached to soluble proteins, or as essential components of ganglioside structures that play a critical role in brain development and neural transmission. Human milk also contains sialic acid conjugated to oligosaccharides, glycolipids, and glycoproteins. These nutrients can reach the large bowel where they may be metabolised by the microbiota. However, little is known about the members of the microbiota involved in this function. To identify intestinal bacteria that utilise sialic acid within a complex intestinal community, we cultured the caecal microbiota from piglets in the presence of 13C-labelled sialic acid. Using RNA-based stable isotope probing, we identified bacteria that consumed 13C-sialic acid by fractionating total RNA in isopycnic buoyant density gradients followed by 16S rRNA gene analysis. Addition of sialic acid caused significant microbial community changes. A relative rise in Prevotella and Lactobacillus species was accompanied by a corresponding reduction in the genera Escherichia/Shigella, Ruminococcus and Eubacterium. Inspection of isotopically labelled RNA sequences suggests that the labelled sialic acid was consumed by a wide range of bacteria. However, species affiliated with the genus Prevotella were clearly identified as the most prolific users, as solely their RNA showed significantly higher relative shares among the most labelled RNA species. Given the relevance of sialic acid in nutrition, this study contributes to a better understanding of their microbial transformation in the intestinal tract with potential implications for human health. View Full-Text
Keywords: Sialic acid; piglet; microbiota; RNA-SIP; Prevotella Sialic acid; piglet; microbiota; RNA-SIP; Prevotella

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Young, W.; Egert, M.; Bassett, S.A.; Bibiloni, R. Detection of Sialic Acid-Utilising Bacteria in a Caecal Community Batch Culture Using RNA-Based Stable Isotope Probing. Nutrients 2015, 7, 2109-2124.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top