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Apoptosis Inducing Effects of Kuding Tea Polyphenols in Human Buccal Squamous Cell Carcinoma Cell Line BcaCD885

1
Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
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Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China
3
Department of Pharmacology, University of Illinois at Chicago, Chicago, IL 60607, USA
4
Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Korea
*
Author to whom correspondence should be addressed.
Nutrients 2014, 6(8), 3084-3100; https://doi.org/10.3390/nu6083084
Received: 29 April 2014 / Revised: 5 July 2014 / Accepted: 23 July 2014 / Published: 5 August 2014
Tea polyphenols are functional substances present in tea. Kuding tea as a traditional drink also contains these compounds. After 25, 50 and 100 μg/mL of Kuding tea polyphenol treatment for 48 h, cell proliferation of human buccal squamous cell carcinoma cell line BcaCD885 was inhibited, and the 100 μg/mL of Kuding tea polyphenol showed the highest inhibitory rate at 72.3%. Compared to the lower concentration, the 100 μg/mL of Kuding tea polyphenols significantly (p < 0.05) induced apoptosis as determined by flow cytometry analysis, the content of sub-G1 cancer cells was 32.7%. By RT-PCR and western blot assays, Kuding tea polyphenol significantly induced apoptosis in BcaCD885 cancer cells (p < 0.05) by upregulating caspase-3, caspase-8, caspase-9, Fas/FasL, Bax, p53, p21, E2F1, p73 and downregulating Bcl-2, Bcl-xL, HIAP-1, and HIAP-2 mRNA and protein expressions. Kuding tea polyphenols thus present apoptosis inducing effects in vitro. View Full-Text
Keywords: Kuding tea; polyphenols; apoptosis; gene; human buccal squamous cell carcinoma cell line BcaCD885 Kuding tea; polyphenols; apoptosis; gene; human buccal squamous cell carcinoma cell line BcaCD885
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Zhao, X.; Pang, L.; Li, J.; Song, J.-L.; Qiu, L.-H. Apoptosis Inducing Effects of Kuding Tea Polyphenols in Human Buccal Squamous Cell Carcinoma Cell Line BcaCD885. Nutrients 2014, 6, 3084-3100.

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