Short-Term Caloric Restriction and Subsequent Re-Feeding Compromise Liver Health and Associated Lipid Mediator Signaling in Aged Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Preparation of Liver Lysates
2.3. Malondialdehyde (MDA) Assay
2.4. Cytokine Quantification
2.5. Proteomics
2.6. Metabololipidomics via UPLC-MS/MS
2.7. Data Handling and Statistical Analysis
3. Results
3.1. Aging Has Marginal Impact on the Hepatic Inflammatory Microenvironment
3.2. Aging Reduces Lipoxygenase-Mediated Oxygenation of Polyunsaturated Fatty Acids
3.3. Caloric Restriction Elicits Alterations in the Hepatic Inflammatory Microenvironment of Old Mice
3.4. CR and Subsequent Re-Feeding Diminish PUFA Metabolism in Aged Mice
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Schädel, P.; Wichmann-Costaganna, M.; Czapka, A.; Gebert, N.; Ori, A.; Werz, O. Short-Term Caloric Restriction and Subsequent Re-Feeding Compromise Liver Health and Associated Lipid Mediator Signaling in Aged Mice. Nutrients 2023, 15, 3660. https://doi.org/10.3390/nu15163660
Schädel P, Wichmann-Costaganna M, Czapka A, Gebert N, Ori A, Werz O. Short-Term Caloric Restriction and Subsequent Re-Feeding Compromise Liver Health and Associated Lipid Mediator Signaling in Aged Mice. Nutrients. 2023; 15(16):3660. https://doi.org/10.3390/nu15163660
Chicago/Turabian StyleSchädel, Patrick, Mareike Wichmann-Costaganna, Anna Czapka, Nadja Gebert, Alessandro Ori, and Oliver Werz. 2023. "Short-Term Caloric Restriction and Subsequent Re-Feeding Compromise Liver Health and Associated Lipid Mediator Signaling in Aged Mice" Nutrients 15, no. 16: 3660. https://doi.org/10.3390/nu15163660