Adiposity Reduction by Cucumis melo var. gaettongchamoe Extract in High-Fat Diet-Induced Obese Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. CG Collection and Extract Preparation
2.2. Animal Study
2.3. HPLC Analysis
2.4. Serum Biochemical Analysis
2.5. Histopathological Analysis
2.6. Quantitative Real Time–Polymerase Chain Reaction (qRT-PCR) Analysis
2.7. Western Blotting Analysis
2.8. Statistical Analysis
3. Results
3.1. Effects of CGWE Treatment on Body Weight and Serum Lipid Profiles in HFD-Induced Obese Mice
3.2. The Inhibitory Effect of CGWE on Abdominal Fat Accumulation in HFD-Induced Obese Mice
3.3. Effects of CGWE on Fat Accumulation and Lipolysis in Hepatic Tissue of HFD-Induced Obese Mice
3.4. Attenuation of CGWE on Inflammatory Response Regulation during Hepatic Steatosis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Forward Primer (5′→3′) | Reverse Primer (3′→5′) |
---|---|---|
CPT1 | GGCAGAGCAGAGGTTCAAGCT | GCCAGCGCCCGWETCAT |
PPARα | TGGCAAAAGGCAAGGAGAAG | CCCTCTACATAGAACTGCAAGGTT T |
PPARγ | GAGTTCATGCTTGTGAAGGATGCAAGG | CATACTCTGTGATCTCTTGCACGWE |
FAS | GATCCTGGAACGWEAGAACACGWEATCTGG | AGACTGTGGAACACGWEGTGGTGGAACC |
C/EBPα | GTGGACAAGAACAGCAACGAGTAC | GGAATCTCCTAGTCCTGGCTTGC |
aP2 | GAACCTGGAAGCTTGTCTCCAGTG | GATGCTCTTCACCTTCCTGTCGTCTGC |
TNF-α | CCTGTAGCCCACGTCGTA | TTGACCTCAGCGWECTGACTTG |
IL-1β | AGGCTTCCTTGTGCAAGTGT | TGAGTGACACTGCCTTCCTG |
IL-6 | CTCTCTGCAAGAGAGTTCCATCCAG | GCTATGGTACTCCAGAAGACCAGAGG |
IL-18 | GTACAAAGACAGTGAAGTAAGAGGACTG | CTCCATCTTGTTGTGTCCTGGAACACGWE |
β-actin | ACGWEGCCAGGTCATCACTATT | CAAGAAGGAAGGCTGGAAAAGA |
Food Intake (g·day−1) | |||||||
---|---|---|---|---|---|---|---|
Group | 7 | 14 | 21 | 28 | 35 | 42 | 49 |
No | 64.3 ± 5.8 | 63.2 ± 4.7 | 59.7 ± 3.8 | 68.5 ± 5.1 | 60.7 ± 5.0 | 67.2 ± 1.8 | 63.0 ± 2.3 |
Ve | 63.0 ± 5.4 | 55.2 ± 6.1 | 63.0 ±4.3 | 57.8 ± 2.2 | 57.8 ± 1.2 | 56.5 ± 2.2 | 58.2 ± 1.7 |
Po | 68.8 ± 5.5 | 62.2 ± 5.2 | 63.0 ± 3.8 | 59.7 ± 4.6 | 59.8 ± 4.5 | 63.5 ± 2.4 | 61.3 ± 3.0 |
L | 63.3 ± 3.1 | 51.7 ± 3.9 | 5608 ± 3.0 | 57.3 ± 3.4 | 54.7 ± 2.8 | 50.5 ± 3.7 | 48.2 ± 3.4 |
H | 62.8 ± 2.8 | 50.5 ± 2.0 | 60.7 ± 3.4 | 54.4 ± 2.9 | 53.5 ± 2.6 | 53.0 ± 4.4 | 52.3 ± 3.2 |
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Park, S.Y.; Kim, J.E.; Kang, H.M.; Song, H.J.; Kang, N.J.; Hwang, D.Y.; Choi, Y.-W. Adiposity Reduction by Cucumis melo var. gaettongchamoe Extract in High-Fat Diet-Induced Obese Mice. Nutrients 2023, 15, 3292. https://doi.org/10.3390/nu15153292
Park SY, Kim JE, Kang HM, Song HJ, Kang NJ, Hwang DY, Choi Y-W. Adiposity Reduction by Cucumis melo var. gaettongchamoe Extract in High-Fat Diet-Induced Obese Mice. Nutrients. 2023; 15(15):3292. https://doi.org/10.3390/nu15153292
Chicago/Turabian StylePark, Sun Young, Ji Eun Kim, He Mi Kang, Hee Jin Song, Nam Jun Kang, Dae Youn Hwang, and Young-Whan Choi. 2023. "Adiposity Reduction by Cucumis melo var. gaettongchamoe Extract in High-Fat Diet-Induced Obese Mice" Nutrients 15, no. 15: 3292. https://doi.org/10.3390/nu15153292