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Article

Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure

1
School of Biomedical Sciences, The University of Queensland, St Lucia, QLD 4072, Australia
2
Child Health Research Centre, The University of Queensland, South Brisbane, QLD 4101, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Robert Colin Carter
Nutrients 2022, 14(9), 1874; https://doi.org/10.3390/nu14091874
Received: 1 April 2022 / Revised: 27 April 2022 / Accepted: 28 April 2022 / Published: 29 April 2022
Prenatal alcohol exposure disturbs fetal and placental growth and can alter DNA methylation (DNAm). Supplementation with the methyl donor choline can increase fetal and placental growth and restore DNAm, suggesting converging effects on one-carbon metabolism (1CM). We investigated the impact of periconceptional ethanol (PCE) exposure and prenatal choline supplementation on 1CM in maternal, placental, and fetal compartments. Female Sprague Dawley rats were given a liquid diet containing 12.5% ethanol (PCE) or 0% ethanol (control) for 4 days before and 4 days after conception. Dams were then placed on chow with different concentrations of choline (1.6 g, 2.6 g, or 7.2 g choline/kg chow). Plasma and tissues were collected in late gestation for the analysis of 1CM components by means of mass spectrometry and real-time PCR. PCE reduced placental components of 1CM, particularly those relating to folate metabolism, resulting in a 3–7.5-fold reduction in the ratio of s-adenosylmethionine:s-adenosylhomocysteine (SAM:SAH) (p < 0.0001). Choline supplementation increased placental 1CM components and the SAM:SAH ratio (3.5–14.5-fold, p < 0.0001). In the maternal and fetal compartments, PCE had little effect, whereas choline increased components of 1CM. This suggests that PCE impairs fetal development via altered placental 1CM, highlighting its role in modulating nutritional inputs to optimize fetal development. View Full-Text
Keywords: methyl group; one carbon metabolism; prenatal alcohol; maternal nutrition; placenta; mass spectrometry; DNA methylation methyl group; one carbon metabolism; prenatal alcohol; maternal nutrition; placenta; mass spectrometry; DNA methylation
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MDPI and ACS Style

Steane, S.E.; Kumar, V.; Cuffe, J.S.M.; Moritz, K.M.; Akison, L.K. Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure. Nutrients 2022, 14, 1874. https://doi.org/10.3390/nu14091874

AMA Style

Steane SE, Kumar V, Cuffe JSM, Moritz KM, Akison LK. Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure. Nutrients. 2022; 14(9):1874. https://doi.org/10.3390/nu14091874

Chicago/Turabian Style

Steane, Sarah E., Vinod Kumar, James S.M. Cuffe, Karen M. Moritz, and Lisa K. Akison. 2022. "Prenatal Choline Supplementation Alters One Carbon Metabolites in a Rat Model of Periconceptional Alcohol Exposure" Nutrients 14, no. 9: 1874. https://doi.org/10.3390/nu14091874

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