The Effect of Exercise Training and Royal Jelly on Hippocampal Cannabinoid-1-Receptors and Pain Threshold in Experimental Autoimmune Encephalomyelitis in Rats as Animal Model of Multiple Sclerosis
Abstract
:1. Introduction
2. Methods
2.1. Animals and Implementation of Research Design
2.2. Induction of EAE Disease
2.3. Training Protocol
2.4. Royal Jelly Supplementation
2.5. Pain Threshold (PT) Evaluation
2.6. Measurement of CB1R
2.7. Data Analysis Procedure
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Sequence of Primers | Product Size (bp) |
---|---|---|
TBP | Forward: 5′-GCGGGGTCATGAAATCCAGT-3′ | 147 |
Reverse: 5′-AGTGATGTGGGGACAAAACGA-3′ | ||
CB1 receptor | Forward: 5′-AGAACTTACTGTGAACAGGCTCT-3′ | 105 |
Reverse: 5′-ACTGAGAAAGAGATGCCGGG-3′ |
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Kheirdeh, M.; Koushkie Jahromi, M.; Brühl, A.B.; Brand, S. The Effect of Exercise Training and Royal Jelly on Hippocampal Cannabinoid-1-Receptors and Pain Threshold in Experimental Autoimmune Encephalomyelitis in Rats as Animal Model of Multiple Sclerosis. Nutrients 2022, 14, 4119. https://doi.org/10.3390/nu14194119
Kheirdeh M, Koushkie Jahromi M, Brühl AB, Brand S. The Effect of Exercise Training and Royal Jelly on Hippocampal Cannabinoid-1-Receptors and Pain Threshold in Experimental Autoimmune Encephalomyelitis in Rats as Animal Model of Multiple Sclerosis. Nutrients. 2022; 14(19):4119. https://doi.org/10.3390/nu14194119
Chicago/Turabian StyleKheirdeh, Maryam, Maryam Koushkie Jahromi, Annette Beatrix Brühl, and Serge Brand. 2022. "The Effect of Exercise Training and Royal Jelly on Hippocampal Cannabinoid-1-Receptors and Pain Threshold in Experimental Autoimmune Encephalomyelitis in Rats as Animal Model of Multiple Sclerosis" Nutrients 14, no. 19: 4119. https://doi.org/10.3390/nu14194119