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Construction and Application of Hepatocyte Model Based on Microfluidic Chip Technique in Evaluating Emodin

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
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Academic Editor: Lynnette Ferguson
Nutrients 2022, 14(13), 2768; https://doi.org/10.3390/nu14132768
Received: 7 June 2022 / Revised: 28 June 2022 / Accepted: 2 July 2022 / Published: 5 July 2022
(This article belongs to the Section Nutrition Methodology & Assessment)
The current cytological evaluation technique of health food raw materials does not entirely meet the needs of evaluating health food. Our study adopted the microfluidic chip technique for the first time to construct a hepatocyte model of evaluating emodin, which was composed of a human hepatocellular carcinoma cell (HepG2) and microfluidic chip. The mixed glue of a model with rat tail collagen type I (1.3 mg/mL) + gelatin (7.5%) was used to simulate the microenvironment of a cell. The validity of this model was evaluated by cell proliferation activity and cell staining, and the toxicity of emodin was evaluated by a series of metabolic indicators on this model. The results indicated that the repeatability of the constructed hepatocyte model was favorable, with a coefficient of variation (CV) of 2.8%. After emodin continuously was exposed for 48 h, the cell inhibition was obvious at 100 and 200 μM, and the number of dead cells gradually increased with the increasing of emodin concentration, and the difference of BUN was significant between the emodin group and blank group (p < 0.05). The constructed model has a favorable applicability in evaluating emodin. This study provides an important platform and a potential in vitro alternative model for assessing and predicting the health effects of health food. View Full-Text
Keywords: microfluidic chip; raw materials of health food; emodin; hepatocyte model; evaluation microfluidic chip; raw materials of health food; emodin; hepatocyte model; evaluation
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MDPI and ACS Style

Chen, D.; Yin, J.; Yang, Z.; Qin, W.; Huo, J.; Huang, J.; Sun, J.; Piao, W. Construction and Application of Hepatocyte Model Based on Microfluidic Chip Technique in Evaluating Emodin. Nutrients 2022, 14, 2768. https://doi.org/10.3390/nu14132768

AMA Style

Chen D, Yin J, Yang Z, Qin W, Huo J, Huang J, Sun J, Piao W. Construction and Application of Hepatocyte Model Based on Microfluidic Chip Technique in Evaluating Emodin. Nutrients. 2022; 14(13):2768. https://doi.org/10.3390/nu14132768

Chicago/Turabian Style

Chen, Di, Jiyong Yin, Zhuo Yang, Wen Qin, Junsheng Huo, Jian Huang, Jing Sun, and Wei Piao. 2022. "Construction and Application of Hepatocyte Model Based on Microfluidic Chip Technique in Evaluating Emodin" Nutrients 14, no. 13: 2768. https://doi.org/10.3390/nu14132768

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