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Article

Phytate and Butyrate Differently Influence the Proliferation, Apoptosis and Survival Pathways in Human Cancer and Healthy Colonocytes

1
Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland
2
Department of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Lynnette Ferguson
Nutrients 2021, 13(6), 1887; https://doi.org/10.3390/nu13061887
Received: 29 April 2021 / Revised: 19 May 2021 / Accepted: 28 May 2021 / Published: 31 May 2021
(This article belongs to the Special Issue Eating Habits and Disease Risk Factors)
The colonic epithelium is never exposed to a single factor, therefore studies on the effect of combinations of factors naturally and persistently present in the intestines are of special importance for understanding the phenomena occurring at this place. The aim of the study was to investigate the combined effect of 1 mM phytate and 1 mM butyrate (PA1B1) on cell lines derived from cancer (HCT116 and HT-29) and healthy (NCM460D) human colonic epithelium. Colorimetric and flow cytometry methods were used to determine the proliferation rate, cell cycle, and apoptosis. Selected markers of proliferation, inflammatory, and survival pathways were investigated at the mRNA and/or protein level. The combination of phytate and butyrate disturbed the cell cycle and triggered apoptosis and/or death in both studied cancer colonocytes to a higher extent compared to healthy colonocytes. Moreover, in healthy colonocytes, phytate activated the survival pathway without stimulation of inflammatory response. This may indicate that the response of healthy colonocytes to phytate protects colonic epithelium from the loss of integrity and tightness that would occur if inflammation developed. Based on the obtained results we postulate that studies on both cancer and/or healthy colonocytes should be carried out in the presence of butyrate as the permanent component of colonic contents. This should be of special importance when anti-proliferative/pro-apoptotic activity or inflammatory status of colonocytes is to be investigated. View Full-Text
Keywords: cancer colonocytes; healthy colonocytes; phytate; butyrate; apoptosis; cell cycle; cell survival; diet cancer colonocytes; healthy colonocytes; phytate; butyrate; apoptosis; cell cycle; cell survival; diet
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MDPI and ACS Style

Markiewicz, L.H.; Ogrodowczyk, A.M.; Wiczkowski, W.; Wróblewska, B. Phytate and Butyrate Differently Influence the Proliferation, Apoptosis and Survival Pathways in Human Cancer and Healthy Colonocytes. Nutrients 2021, 13, 1887. https://doi.org/10.3390/nu13061887

AMA Style

Markiewicz LH, Ogrodowczyk AM, Wiczkowski W, Wróblewska B. Phytate and Butyrate Differently Influence the Proliferation, Apoptosis and Survival Pathways in Human Cancer and Healthy Colonocytes. Nutrients. 2021; 13(6):1887. https://doi.org/10.3390/nu13061887

Chicago/Turabian Style

Markiewicz, Lidia H., Anna M. Ogrodowczyk, Wiesław Wiczkowski, and Barbara Wróblewska. 2021. "Phytate and Butyrate Differently Influence the Proliferation, Apoptosis and Survival Pathways in Human Cancer and Healthy Colonocytes" Nutrients 13, no. 6: 1887. https://doi.org/10.3390/nu13061887

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