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Article

Methylation and Expression of FTO and PLAG1 Genes in Childhood Obesity: Insight into Anthropometric Parameters and Glucose–Lipid Metabolism

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Department of Pediatric Oncology and Hematology, University Children’s Hospital of Krakow, 30-663 Krakow, Poland
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Department of Pediatric Oncology and Hematology, Faculty of Medicine, Jagiellonian University Medical College, 30-663 Krakow, Poland
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Center for Medical Genomics—OMICRON, Jagiellonian University Medical College, 30-663 Krakow, Poland
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Department of Pediatric and Adolescent Endocrinology, Faculty of Medicine, Jagiellonian University Medical College, 30-663 Krakow, Poland
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Department of Medical Genetics, Faculty of Medicine, Jagiellonian University Medical College, 30-663 Krakow, Poland
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Department of Clinical Biochemistry, Faculty of Medicine, Jagiellonian University Medical College, 30-663 Krakow, Poland
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Department of Pediatrics, Gastroenterology and Nutrition, Faculty of Medicine, Jagiellonian University Medical College, 30-663 Krakow, Poland
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Student Scientific Group of Pediatric Oncology and Hematology, Jagiellonian University Medical College, 30-663 Krakow, Poland
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Author to whom correspondence should be addressed.
Academic Editor: Peter Henneman
Nutrients 2021, 13(5), 1683; https://doi.org/10.3390/nu13051683
Received: 20 March 2021 / Revised: 23 April 2021 / Accepted: 11 May 2021 / Published: 15 May 2021
(This article belongs to the Section Nutrigenetics and Nutrigenomics)
The occurrence of childhood obesity is influenced by both genetic and epigenetic factors. FTO (FTO alpha-ketoglutarate dependent dioxygenase) is a gene of well-established connection with adiposity, while a protooncogene PLAG1 (PLAG1 zinc finger) has been only recently linked to this condition. We performed a cross-sectional study on a cohort of 16 obese (aged 6.6–17.7) and 10 healthy (aged 11.4–16.9) children. The aim was to evaluate the relationship between methylation and expression of the aforementioned genes and the presence of obesity as well as alterations in anthropometric measurements (including waist circumference (WC), body fat (BF_kg) and body fat percent (BF_%)), metabolic parameters (lipid profile, blood glucose and insulin levels, presence of insulin resistance) and blood pressure. Expression and methylation were measured in peripheral blood mononuclear cells using a microarray technique and a method based on restriction enzymes, respectively. Multiple regression models were constructed to adjust for the possible influence of age and sex on the investigated associations. We showed significantly increased expression of the FTO gene in obese children and in patients with documented insulin resistance. Higher FTO expression was also associated with an increase in WC, BF_kg, and BF_% as well as higher fasting concentration of free fatty acids (FFA). FTO methylation correlated positively with WC and BF_kg. Increase in PLAG1 expression was associated with higher BF%. Our results indicate that the FTO gene is likely to play an important role in the development of childhood adiposity together with coexisting impairment of glucose-lipid metabolism. View Full-Text
Keywords: epigenetics; expression; FTO; PLAG1; obesity; insulin resistance; children epigenetics; expression; FTO; PLAG1; obesity; insulin resistance; children
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MDPI and ACS Style

Czogała, W.; Czogała, M.; Strojny, W.; Wątor, G.; Wołkow, P.; Wójcik, M.; Bik Multanowski, M.; Tomasik, P.; Wędrychowicz, A.; Kowalczyk, W.; Miklusiak, K.; Łazarczyk, A.; Hałubiec, P.; Skoczeń, S. Methylation and Expression of FTO and PLAG1 Genes in Childhood Obesity: Insight into Anthropometric Parameters and Glucose–Lipid Metabolism. Nutrients 2021, 13, 1683. https://doi.org/10.3390/nu13051683

AMA Style

Czogała W, Czogała M, Strojny W, Wątor G, Wołkow P, Wójcik M, Bik Multanowski M, Tomasik P, Wędrychowicz A, Kowalczyk W, Miklusiak K, Łazarczyk A, Hałubiec P, Skoczeń S. Methylation and Expression of FTO and PLAG1 Genes in Childhood Obesity: Insight into Anthropometric Parameters and Glucose–Lipid Metabolism. Nutrients. 2021; 13(5):1683. https://doi.org/10.3390/nu13051683

Chicago/Turabian Style

Czogała, Wojciech, Małgorzata Czogała, Wojciech Strojny, Gracjan Wątor, Paweł Wołkow, Małgorzata Wójcik, Mirosław Bik Multanowski, Przemysław Tomasik, Andrzej Wędrychowicz, Wojciech Kowalczyk, Karol Miklusiak, Agnieszka Łazarczyk, Przemysław Hałubiec, and Szymon Skoczeń. 2021. "Methylation and Expression of FTO and PLAG1 Genes in Childhood Obesity: Insight into Anthropometric Parameters and Glucose–Lipid Metabolism" Nutrients 13, no. 5: 1683. https://doi.org/10.3390/nu13051683

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