The following are available online at
https://www.mdpi.com/2072-6643/13/2/377/s1, Table S1. Previous crops, planting and harvesting dates, crop protection and fertilization regimes used for the barley, potato, carrot and onion feed crop cultivation; Table S2. Effect of crop protection and fertilization on the chemical composition of the experimental rat feeds (per 100g fresh weight); Table S3. Effect of crop protection and fertilization on the daily intake of macronutrients, antioxidants, contaminants and minerals in the first and second generation of rats; Table S3 continued. Effect of crop protection and fertilization on the daily intake of macronutrients, antioxidants, contaminants and minerals inthe first and second generation of rats; Table S4. Results of the 3-factor ANOVA of effects of crop protection(P), fertilization(F)and generation (G)on (a)growth, basic physiological, endocrine and immunological parameters and (b)dietary macronutrient, antioxidant and contaminant intake in rats; Table S4 continued. Results (
p-values) of the 3-factor ANOVA of effects of crop protection, fertilization and generation on (a)growth, basic physiological, endocrine and immunological parametersand (b)dietary macronutrient, antioxidant andcontaminant intake in rats; Table S5. Rat body composition and basic physiological parameters in the first and second generation of rats; Table S6. Effect of crop protection and fertilization on plasma hormones, immunoglobulin concentrations and immune system responsiveness in the first and second generation of rats Figure S1 Experimental design flow diagram. * animals assessed in trials; Figure S2. Effects of organic crop protection (OP) or conventional crop protection (CP) and organic fertility management (OF) or conventional fertility management (CF) on the daily intake of protein, lipid, fiber, ash, Cu, polyphenols, flavonoids, lutein, β-carotene, antioxidant capacity (TEAC) of the first (G1) and second (G2) generation of rats. Results shown as means ±SEM of
n = 24 animals, different letters above bars indicate significant difference (
p ≤ 0.05) determined by Tukey’s HSD test. * two factor ANOVA detected a significant interaction between crop protection and fertilization regimes (see Table S3 for ANOVA
p-values and main effect means); Figure S3. Effects of organic crop protection (OP) or conventional crop protection (CP) and organic fertility management (OF) or conventional fertility management (CF) on the daily intake of cadmium (Cd) and lead (Pb) of the first (G1) and second (G2) generation of rats. Results shown as means ±SEM of
n = 24 animals, different letters above bars indicate significant difference (
p ≤ 0.05) determined by Tukey’sHSD test. * two factor ANOVA detected a significant interaction between crop protection and fertilization regimes (see Table S3 for ANOVA
p-values and main effect means); Figure S4. Correlation between concentration of experimental feed components and physiological parametersfor the first generation of rats (G1). The Pearson correlation was indicated on the color scale(blue as positive and red as negative), where color intensity and circle sizes are proportional to the correlation coefficient. PRO, protein; LIP, lipids; FIB, fiber; ASH, ash; POL, polyphenols; FLA, flavonols; LUT, lutein; BCA, β-carotene; TEAC, feed antioxidant activity; Cu, copper; ALD, aldicarb;DIQ, diquat; Cd, cadmium; Ni, nickel; Pb, lead; CONTAM, contaminants altogether; IBW.G1, initial body weightof first generation of rats; IBW.G2, initial body weight of second generation of rats;int, total feed intake; twg, total weight gain; FCR, feed conversion ratio; %BDM, percent of body dry matter; rbc, red blood cells count; hb, blood hemoglobin content; glu, plasma glucose content; teac, plasma antioxidant capacity; cs, corticosterone; gh, growth hormone; igf, insulin-like growth factor 1; ins, insulin; lep, leptin; ts, testosterone; iga, immunoglobulin A; igg, immunoglobulin G; sp-lp, spontaneous lymphocyte proliferation; cona-lp, concanavalin A-stimulated lymphocyte proliferation; lps-lp, lipopolysaccharide-stimulated lymphocyte proliferation; Figure S5. Correlation between concentration of experimental feed components and physiological parameters for the second generation of rats (G2). The Pearson correlation was indicated on the color scale (blue as positive and red as negative), where color intensity and circle sizes are proportional to the correlation coefficient. PRO, protein; LIP, lipids; FIB, fiber; ASH, ash; POL, polyphenols; FLA, flavonols; LUT, lutein; BCA, β-carotene; TEAC, feed antioxidant activity; Cu, copper; ALD, aldicarb; DIQ, diquat; Cd, cadmium; Ni, nickel; Pb, lead; CONTAM, contaminants altogether; IBW.G2, initial body weight of second generationof rats; int, total feed intake; twg, total weight gain; FCR, feed conversion ratio; %BDM, percent of body dry matter; rbc, red blood cells count; hb, blood hemoglobin content; glu, plasma glucose content; teac, plasma antioxidant capacity; cs, corticosterone; gh, growth hormone; igf, insulin-like growth factor 1; ins, insulin; lep, leptin; ts, testosterone; iga, immunoglobulin A; igg, immunoglobulin G; sp-lp, spontaneous lymphocyte proliferation; cona-lp, concanavalin A-stimulated lymphocyte proliferation; lps-lp, lipopolysaccharide-stimulated lymphocyte proliferation; Figure S6. Correlation between concentration of experimental feed components and physiological parameters for both generations of rats (G1+G2). The Pearson correlation was indicated on the color scale (blue as positive and red as negative),where color intensity and circle sizes are proportional to the correlation coefficient. PRO, protein; LIP, lipids; FIB, fiber; ASH, ash; POL, polyphenols; FLA, flavonols; LUT, lutein; BCA, β-carotene; TEAC, feed antioxidant activity; Cu, copper; ALD, aldicarb; DIQ, diquat; Cd, cadmium; Ni, nickel; Pb, lead; CONTAM, contaminants altogether; IBW.G1, initial body weight of first generation of rats; IBW, initial body weight of rats; int, total feed intake; twg, total weight gain; FCR, feed conversion ratio; %BDM, percent of body dry matter; rbc, red blood cells count; hb, blood hemoglobin content; glu, plasma glucose content; teac, plasma antioxidant capacity; cs, corticosterone; gh, growth hormone; igf, insulin-like growth factor 1; ins, insulin; lep, leptin; ts, testosterone; iga, immunoglobulin A; igg, immunoglobulin G; sp-lp, spontaneous lymphocyte proliferation; cona-lp, concanavalin A-stimulated lymphocyte proliferation; lps-lp, lipopolysaccharide-stimulated lymphocyte proliferation; Figure S7. Correlation between calculated intake of experimental feed components and physiological parameters for the first generation of rats (G1). The Pearson correlation was indicated on the color scale (blue as positive and red as negative), where color intensity and circle sizes are proportional to the correlation coefficient. PRO, protein; LIP, lipids; FIB, fiber; ASH, ash; POL, polyphenols; FLA, flavonols; LUT, lutein; BCA, β-carotene; TEAC, feed antioxidant activity; Cu, copper; ALD, aldicarb; DIQ, diquat; Cd, cadmium; Ni, nickel; Pb, lead; CONTAM, contaminants altogether; IBW.G1, initial body weight of first generation of rats; IBW.G2, initial body weight of second generation of rats; int, total feed intake; twg, total weight gain; FCR, feed conversion ratio; %BDM, percent of body dry matter; rbc, red blood cells count; hb, blood hemoglobin content; glu, plasma glucose content; teac, plasma antioxidant capacity; cs, corticosterone; gh, growth hormone; igf, insulin-like growth factor 1; ins, insulin; lep, leptin; ts, testosterone; iga, immunoglobulin A; igg, immunoglobulin G; sp-lp, spontaneous lymphocyte proliferation; cona-lp, concanavalin A-stimulated lymphocyte proliferation; lps-lp, lipopolysaccharide-stimulated lymphocyte proliferation; Figure S8. Correlation between calculated intake of experimental feed components and physiological parameters for the second generation of rats (G2). The Pearson correlation was indicated on the color scale (blue as positive and red as negative), where color intensity and circle sizes are proportional to the correlation coefficient. PRO, protein; LIP, lipids; FIB, fiber; ASH, ash; POL, polyphenols; FLA, flavonols; LUT, lutein; BCA, β-carotene; TEAC, feed antioxidant activity; Cu, copper; ALD, aldicarb; DIQ, diquat; Cd, cadmium; Ni, nickel; Pb, lead; CONTAM, contaminants altogether; IBW.G2, initial body weight of second generationof rats; int, total feed intake; twg, total weight gain; FCR, feed conversion ratio; %BDM, percent of body dry matter; rbc, red blood cells count; hb, blood hemoglobin content; glu, plasma glucose content; teac, plasma antioxidant capacity; cs, corticosterone; gh, growth hormone; igf, insulin-like growth factor 1; ins, insulin; lep, leptin; ts, testosterone; iga, immunoglobulin A; igg, immunoglobulin G; sp-lp, spontaneous lymphocyte proliferation; cona-lp, concanavalin A-stimulated lymphocyte proliferation; lps-lp, lipopolysaccharide-stimulated lymphocyte proliferation; Figure S9. Correlation between calculated intake of experimental feed components and physiological parameters for both generations of rats (G1+G2). The Pearson correlation was indicatedon the color scale (blue as positive and red as negative), where color intensity and circle sizes are proportional to the correlation coefficient. PRO, protein; LIP, lipids; FIB, fiber; ASH, ash; POL, polyphenols; FLA, flavonols; LUT, lutein; BCA, β-carotene; TEAC, feed antioxidant activity; Cu, copper; ALD, aldicarb; DIQ, diquat; Cd, cadmium; Ni, nickel; Pb, lead; CONTAM, contaminants altogether; IBW.G1, initial body weight of first generation of rats; IBW, initial body weight of rats; int, total feed intake; twg, total weight gain; FCR, feed conversion ratio; %BDM, percent of body dry matter; rbc, red blood cells count; hb, blood hemoglobin content; glu, plasma glucose content; teac, plasma antioxidant capacity; cs, corticosterone; gh, growth hormone; igf, insulin-like growth factor 1; ins, insulin; lep, leptin; ts, testosterone; iga, immunoglobulin A; igg, immunoglobulin G; sp-lp, spontaneous lymphocyte proliferation; cona-lp, concanavalin A-stimulated lymphocyte proliferation; lps-lp, lipopolysaccharide-stimulated lymphocyte proliferation.