Next Article in Journal
The Feasibility of Using Computrition Software for Nutrition Research—A Pilot Study
Previous Article in Journal
The Use of Lower or Higher Than Recommended Doses of Folic Acid Supplements during Pregnancy Is Associated with Child Attentional Dysfunction at 4–5 Years of Age in the INMA Project
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
Journals
Nutrients
Volume 13
Issue 2
10.3390/nu13020328
nutrients-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

share announcement thumb_up
...
textsms
...
Open AccessArticle

Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro

by
Claudio Salaris
1,†,
Melania Scarpa
2,†,
Marina Elli
3,
Alice Bertolini
1,
Simone Guglielmetti
4,
Fabrizio Pregliasco
5,
Corrado Blandizzi
6,
Paola Brun
1,* and
Ignazio Castagliuolo
1
1
Department of Molecular Medicine, University of Padua, 35121 Padua, Italy
2
Laboratory of Advanced Translational Research, Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy
3
AAT-Advanced Analytical Technologies S.r.l., Fiorenzuola d’Arda, 29122 Piacenza, Italy
4
Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, 20122 Milan, Italy
5
IRCCS Istituto Ortopedico Galeazzi, University of Milan, 20136 Milan, Italy
6
Unit of Pharmacology and Pharmacovigilance, Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, Italy
*
Author to whom correspondence should be addressed.
†
Equally contributed to the work.
Nutrients 2021, 13(2), 328; https://doi.org/10.3390/nu13020328
Received: 16 December 2020 / Revised: 20 January 2021 / Accepted: 21 January 2021 / Published: 23 January 2021
(This article belongs to the Section Nutrition and Public Health)
View Full-Text Download PDF
Browse Figures

Abstract

SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection. View Full-Text
Keywords: lactoferrin; SARS-CoV-2; antiviral immunity; COVID-19 lactoferrin; SARS-CoV-2; antiviral immunity; COVID-19
►▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

SciFeed

Share and Cite

MDPI and ACS Style

Salaris, C.; Scarpa, M.; Elli, M.; Bertolini, A.; Guglielmetti, S.; Pregliasco, F.; Blandizzi, C.; Brun, P.; Castagliuolo, I. Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro. Nutrients 2021, 13, 328. https://doi.org/10.3390/nu13020328

AMA Style

Salaris C, Scarpa M, Elli M, Bertolini A, Guglielmetti S, Pregliasco F, Blandizzi C, Brun P, Castagliuolo I. Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro. Nutrients. 2021; 13(2):328. https://doi.org/10.3390/nu13020328

Chicago/Turabian Style

Salaris, Claudio; Scarpa, Melania; Elli, Marina; Bertolini, Alice; Guglielmetti, Simone; Pregliasco, Fabrizio; Blandizzi, Corrado; Brun, Paola; Castagliuolo, Ignazio. 2021. "Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro" Nutrients 13, no. 2: 328. https://doi.org/10.3390/nu13020328

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop