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Article

Effects of Human Milk Oligosaccharides on the Adult Gut Microbiota and Barrier Function

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Quadram Institute Bioscience, Gut Microbes and Health Institute Strategic Programme, Norwich Research Park, Norwich NR4 7UQ, UK
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Glycom A/S, Kogle Allé 4, DK-2970 Hørsholm, Denmark
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Prodigest Bv, B-9052 Gent, Belgium
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Emulate Inc., 27 Drydock Ave, Boston, MA 02210, USA
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Graduate Program, Department of Medicine, School of Health Sciences, National and Kapodistrian University of Athens, 11527 Athens, Greece
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Quadram Institute Bioscience, Core Sciences Resources, Norwich Research Park, Norwich NR5 7UQ, UK
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Author to whom correspondence should be addressed.
Nutrients 2020, 12(9), 2808; https://doi.org/10.3390/nu12092808
Received: 23 July 2020 / Revised: 3 September 2020 / Accepted: 7 September 2020 / Published: 13 September 2020
Human milk oligosaccharides (HMOs) shape the gut microbiota in infants by selectively stimulating the growth of bifidobacteria. Here, we investigated the impact of HMOs on adult gut microbiota and gut barrier function using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®), Caco2 cell lines, and human intestinal gut organoid-on-chips. We showed that fermentation of 2’-O-fucosyllactose (2’FL), lacto-N-neotetraose (LNnT), and combinations thereof (MIX) led to an increase of bifidobacteria, accompanied by an increase of short chain fatty acid (SCFA), in particular butyrate with 2’FL. A significant reduction in paracellular permeability of FITC-dextran probe was observed using Caco2 cell monolayers with fermented 2’FL and MIX, which was accompanied by an increase in claudin-8 gene expression as shown by qPCR, and a reduction in IL-6 as determined by multiplex ELISA. Using gut-on-chips generated from human organoids derived from proximal, transverse, and distal colon biopsies (Colon Intestine-Chips), we showed that claudin-5 was significantly upregulated across all three gut-on-chips following treatment with fermented 2’FL under microfluidic conditions. Taken together, these data show that, in addition to their bifidogenic activity, HMOs have the capacity to modulate immune function and the gut barrier, supporting the potential of HMOs to provide health benefits in adults. View Full-Text
Keywords: human milk oligosaccharides; adult gut microbiota; gut barrier function; gut-on-chips; SHIME® human milk oligosaccharides; adult gut microbiota; gut barrier function; gut-on-chips; SHIME®
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MDPI and ACS Style

Šuligoj, T.; Vigsnæs, L.K.; Abbeele, P.V.d.; Apostolou, A.; Karalis, K.; Savva, G.M.; McConnell, B.; Juge, N. Effects of Human Milk Oligosaccharides on the Adult Gut Microbiota and Barrier Function. Nutrients 2020, 12, 2808. https://doi.org/10.3390/nu12092808

AMA Style

Šuligoj T, Vigsnæs LK, Abbeele PVd, Apostolou A, Karalis K, Savva GM, McConnell B, Juge N. Effects of Human Milk Oligosaccharides on the Adult Gut Microbiota and Barrier Function. Nutrients. 2020; 12(9):2808. https://doi.org/10.3390/nu12092808

Chicago/Turabian Style

Šuligoj, Tanja, Louise K. Vigsnæs, Pieter V.d. Abbeele, Athanasia Apostolou, Katia Karalis, George M. Savva, Bruce McConnell, and Nathalie Juge. 2020. "Effects of Human Milk Oligosaccharides on the Adult Gut Microbiota and Barrier Function" Nutrients 12, no. 9: 2808. https://doi.org/10.3390/nu12092808

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