Saturated Fatty Acids Promote GDF15 Expression in Human Macrophages through the PERK/eIF2/CHOP Signaling Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of FFA Solutions
2.2. Cell Culture and Treatments
2.3. siRNA Transfection
2.4. Cell Viability Assay
2.5. Luminex Assay
2.6. RT-qPCR Analysis
2.7. Western Blot Analysis
2.8. ChIP-qPCR Analysis
2.9. Statistical Analyses
3. Results
3.1. SFAs Promote GDF15 Expression and Secretion in Macrophages
3.2. ER Stress, but Not p53, Is Involved in SFA-Induced GDF15 Expression
3.3. PERK/eIF2/CHOP Pathway Regulates SFA-Induced GDF15 Expression
3.4. UFAs Inhibit SFA-Induced ER Stress and GDF15 Expression
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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L’homme, L.; Sermikli, B.P.; Staels, B.; Piette, J.; Legrand-Poels, S.; Dombrowicz, D. Saturated Fatty Acids Promote GDF15 Expression in Human Macrophages through the PERK/eIF2/CHOP Signaling Pathway. Nutrients 2020, 12, 3771. https://doi.org/10.3390/nu12123771
L’homme L, Sermikli BP, Staels B, Piette J, Legrand-Poels S, Dombrowicz D. Saturated Fatty Acids Promote GDF15 Expression in Human Macrophages through the PERK/eIF2/CHOP Signaling Pathway. Nutrients. 2020; 12(12):3771. https://doi.org/10.3390/nu12123771
Chicago/Turabian StyleL’homme, Laurent, Benan Pelin Sermikli, Bart Staels, Jacques Piette, Sylvie Legrand-Poels, and David Dombrowicz. 2020. "Saturated Fatty Acids Promote GDF15 Expression in Human Macrophages through the PERK/eIF2/CHOP Signaling Pathway" Nutrients 12, no. 12: 3771. https://doi.org/10.3390/nu12123771