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Open AccessArticle

Blends of Human Milk Oligosaccharides Confer Intestinal Epithelial Barrier Protection In Vitro

1
Department of Gastrointestinal Health, Nestlé Research, 1000 Lausanne (Vaud), Switzerland
2
Clinical Development Unit, Nestlé Research, 1000 Lausanne (Vaud), Switzerland
*
Authors to whom correspondence should be addressed.
Nutrients 2020, 12(10), 3047; https://doi.org/10.3390/nu12103047
Received: 24 August 2020 / Revised: 29 September 2020 / Accepted: 2 October 2020 / Published: 5 October 2020
Breastfeeding is integral in the proper maturation of the intestinal barrier and protection against inflammatory diseases. When human milk (HM) is not available, supplementation with HM bioactives like Human Milk Oligosaccharides (HMOs) may help in providing breastfeeding barrier-protective benefits. An increasing HMO variety is becoming industrially available, enabling approaching the HMO complexity in HM. We aimed at assessing the impact of blends of available HMOs on epithelial barrier function in vitro. The capacity of individual [2′-Fucosyllactose (2′FL), Difucosyllactose, Lacto-N-tetraose, Lacto-N-neotetraose, 3′-Siallylactose and 6′-Siallylactose] or varying combinations of 3, 5 and 6 HMOs to modulate fluorescein-isothiocyanate (FITC)-labelled Dextran 4 KDa (FD4) translocation and/or transepithelial resistance (TEER) was characterized in Caco-2: HT29- methotrexate (MTX) cell line monolayers before and after an inflammatory challenge with TNF-α and IFN-γ. The six HMO blend (HMO6) dose-dependently limited the cytokine-induced FD4 translocation and TEER decrease and increased TEER values before challenge. Similarly, 3 and 5 HMO blends conferred a significant protection against the challenge, with 2′FL, one of the most abundant but most variable oligosaccharides in HM, being a key contributor. Overall, our results suggest differential ability of specific HMOs in modulating the intestinal barrier and support the potential of supplementation with combinations of available HMOs to promote gut health and protect against intestinal inflammatory disorders. View Full-Text
Keywords: human milk oligosaccharides; Caco-2:HT29-MTX cultures; intestinal barrier function; permeability; inflammation human milk oligosaccharides; Caco-2:HT29-MTX cultures; intestinal barrier function; permeability; inflammation
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Natividad, J.M.; Rytz, A.; Keddani, S.; Bergonzelli, G.; Garcia-Rodenas, C.L. Blends of Human Milk Oligosaccharides Confer Intestinal Epithelial Barrier Protection In Vitro. Nutrients 2020, 12, 3047.

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