Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Oral Glucose Tolerance Test
2.3. Tissue Sampling
2.4. Biochemical Analysis
2.5. Liver Lipid Quantification
2.6. Citrate Synthase Activity Assay
2.7. Histological Analyses
2.8. RNA Preparation and Real-Time qPCR Analysis
2.9. Gut Microbiota Analysis
2.10. Bacterial DNA Sequencing
2.11. Statistical Analysis
3. Results
3.1. Rhubarb Root Extract Prevents Obesity and Fat Mass Accumulation in Diet-Induced Obese Mice
3.2. Rhubarb Root Extract Blunts Glucose Intolerance, Hepatic Steatosis and Liver Inflammation
3.3. Rhubarb Root Extract Protects Against Adiposity and Adipose Tissue Inflammation
3.4. Rhubarb Root Extract Affects Antimicrobial Peptides and Intestinal Renewal
3.5. Effects of Rhubarb Root Extract on Gut Microbiota Composition
3.6. Rhubarb Root Extract Promotes the Growth of Akkermansia muciniphila in HFHS Fed Mice
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Régnier, M.; Rastelli, M.; Morissette, A.; Suriano, F.; Le Roy, T.; Pilon, G.; Delzenne, N.M.; Marette, A.; Van Hul, M.; Cani, P.D. Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice. Nutrients 2020, 12, 2932. https://doi.org/10.3390/nu12102932
Régnier M, Rastelli M, Morissette A, Suriano F, Le Roy T, Pilon G, Delzenne NM, Marette A, Van Hul M, Cani PD. Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice. Nutrients. 2020; 12(10):2932. https://doi.org/10.3390/nu12102932
Chicago/Turabian StyleRégnier, Marion, Marialetizia Rastelli, Arianne Morissette, Francesco Suriano, Tiphaine Le Roy, Geneviève Pilon, Nathalie M Delzenne, André Marette, Matthias Van Hul, and Patrice D Cani. 2020. "Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased Akkermansia muciniphila in Mice" Nutrients 12, no. 10: 2932. https://doi.org/10.3390/nu12102932