Methionine Restriction Partly Recapitulates the Sympathetically Mediated Enhanced Energy Expenditure Induced by Total Amino Acid Restriction in Rats

Spring, Shelby; Singh, Arashdeep; Zapata, Rizaldy C.; Chelikani, Prasanth K.; Pezeshki, Adel

doi:10.3390/nu11030707

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Methionine Restriction Partly Recapitulates the Sympathetically Mediated Enhanced Energy Expenditure Induced by Total Amino Acid Restriction in Rats

Shelby Spring

¹,

Arashdeep Singh

^2,†

Rizaldy C. Zapata

^2,‡,

Prasanth K. Chelikani

^2,3,*

and

Adel Pezeshki

^1,*

Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74078, USA

Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada

Gastrointestinal Research Group, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB T2N 4N1, Canada

Authors to whom correspondence should be addressed.

^†

Present address: Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA.

^‡

Present address: Department of Medicine, School of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.

Nutrients 2019, 11(3), 707; https://doi.org/10.3390/nu11030707

Received: 3 February 2019 / Revised: 19 March 2019 / Accepted: 22 March 2019 / Published: 26 March 2019

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Abstract

Total amino acid (AA) restriction promotes hyperphagia and energy expenditure. We determined whether (i) methionine restriction mimics the effects of total AA restriction, (ii) methionine supplementation attenuates these responses, and iii) sympathetic signaling mediates such effects. Rats were injected with either vehicle (V) or 6-hydroxydopamine (S) to induce chemical sympathectomy, and then randomized to four diets: 16% AA (16AA), 5% AA (5AA), 16% AA-methionine (16AA-Met), and 5% AA+methionine (5AA+Met). Propranolol or ondansetron were injected to examine the role of sympathetic and serotonergic signaling, respectively. 5AA, 5AA+Met, and 16AA-Met increased the food conversion rate for 1–3 weeks in the V and S groups, and increased mean energy expenditure in V group,; the magnitude of these changes was attenuated in the S group. Propranolol decreased the energy expenditure of V16AA, V5AA, and V5AA+Met and of S5AA, S5AA+Met, and S16AA-Met, whereas ondansetron decreased the energy expenditure in only the S groups. Compared to 16AA, the other V groups had reduced body weights from days 7–11 onwards and decreased lean masses throughout the study and the other S groups had decreased body weights and lean masses from day 14 onwards. Total AA restriction enhanced the energy expenditure and reduced the weight and lean mass; these effects were partly recapitulated by methionine restriction and were sympathetically mediated. View Full-Text

Keywords: amino acid restriction; methionine restriction; sympathetic system; energy balance; obesity; obesity-prone rats; 6-hydroxydopamine; energy expenditure

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Spring, S.; Singh, A.; Zapata, R.C.; Chelikani, P.K.; Pezeshki, A. Methionine Restriction Partly Recapitulates the Sympathetically Mediated Enhanced Energy Expenditure Induced by Total Amino Acid Restriction in Rats. Nutrients 2019, 11, 707. https://doi.org/10.3390/nu11030707

AMA Style

Spring S, Singh A, Zapata RC, Chelikani PK, Pezeshki A. Methionine Restriction Partly Recapitulates the Sympathetically Mediated Enhanced Energy Expenditure Induced by Total Amino Acid Restriction in Rats. Nutrients. 2019; 11(3):707. https://doi.org/10.3390/nu11030707

Chicago/Turabian Style

Spring, Shelby, Arashdeep Singh, Rizaldy C. Zapata, Prasanth K. Chelikani, and Adel Pezeshki. 2019. "Methionine Restriction Partly Recapitulates the Sympathetically Mediated Enhanced Energy Expenditure Induced by Total Amino Acid Restriction in Rats" Nutrients 11, no. 3: 707. https://doi.org/10.3390/nu11030707

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Methionine Restriction Partly Recapitulates the Sympathetically Mediated Enhanced Energy Expenditure Induced by Total Amino Acid Restriction in Rats

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