Next Article in Journal
Frequency of Trying to Lose Weight and Its Association with Children’s Weight Perception and Dietary Intake (NHANES 2011–2012)
Previous Article in Journal
Bone Benefits of Fish Oil Supplementation Depend on its EPA and DHA Content
Open AccessArticle

Curcumin Ameliorates Nonalcoholic Fatty Liver Disease through Inhibition of O-GlcNAcylation

School of Life Science and Biotechnology, BK21 Plus KNU Creative BioResearch Group, College of Natural Science, Kyungpook National University, Daegu 41566, Korea
*
Author to whom correspondence should be addressed.
Da Eun Lee and Su Jin Lee and contributed equally to this work.
Current address: Oh-Shin Kwon, School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daegu 41566, Korea.
Nutrients 2019, 11(11), 2702; https://doi.org/10.3390/nu11112702
Received: 25 September 2019 / Revised: 31 October 2019 / Accepted: 4 November 2019 / Published: 8 November 2019
The cause of progression to non-alcoholic fatty liver disease (NAFLD) is not fully understood. In the present study, we aimed to investigate how curcumin, a natural phytopolyphenol pigment, ameliorates NAFLD. Initially, we demonstrated that curcumin dramatically suppresses fat accumulation and hepatic injury induced in methionine and choline-deficient (MCD) diet mice. The severity of hepatic inflammation was alleviated by curcumin treatment. To identify the proteins involved in the pathogenesis of NAFLD, we also characterized the hepatic proteome in MCD diet mice. As a result of two-dimensional proteomic analysis, it was confirmed that thirteen proteins including antioxidant protein were differentially expressed in hepatic steatosis. However, the difference in expression was markedly improved by curcumin treatment. Interestingly, eight of the identified proteins are known to undergo O-GlcNAcylation modification. Thus, we further focused on elucidating how the regulation of O-linked β-N-acetylglucosamine (O-GlcNAc) modification is associated with the progression of hepatic steatosis leading to hepatitis in MCD diet mice. In parallel with lipid accumulation and inflammation, the MCD diet significantly up-regulated hexosamine biosynthetic pathway (HBP) and O-GlcNAc transferase (OGT) via ER stress. Curcumin treatment alleviates the severity of hepatic steatosis by relieving the dependence of O-GlcNAcylation on nuclear factor-κB (NF-κB) in inflammation signaling. Conversely, the expressions of superoxide dismutase 1 (SOD1) and SIRT1 were significantly upregulated by curcumin treatment. In conclusion, curcumin inhibits O-GlcNAcylation pathway, leading to antioxidant responses in non-alcoholic steatohepatitis (NASH) mice. Therefore, curcumin will be a promising therapeutic agent for diseases involving hyper-O-GlcNAcylation, including cancer. View Full-Text
Keywords: liver; O-GlcNAcylation; curcumin; NAFLD and SOD1 liver; O-GlcNAcylation; curcumin; NAFLD and SOD1
Show Figures

Figure 1

MDPI and ACS Style

Lee, D.E.; Lee, S.J.; Kim, S.J.; Lee, H.-S.; Kwon, O.-S. Curcumin Ameliorates Nonalcoholic Fatty Liver Disease through Inhibition of O-GlcNAcylation. Nutrients 2019, 11, 2702.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop